Actin Network as a Normal Component of the Cytoskeleton in Many Vascular Plant Cells

Parthasarathy, M. V.; Perdue, Tony D.; Witztum, A.; Alvernaz, Jennie

doi:10.1002/j.1537-2197.1985.tb08386.x

Cited by 107 publications

(31 citation statements)

References 21 publications

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“…However, details on the association between actin filaments and the nucleus are not yet known. The use of fluorescent-labelled phallotoxins revealed that actin filaments were present near the nucleus in the cells of various plants [2,10,11] including statocytes [5]. However, it was not easy to discern a physical connection.…”

Section: Discussionmentioning

confidence: 99%

Actin filaments responsible for the location of the nucleus in the lentil statocyte are sensitive to gravity

Lorenzi

Perbal

1990

Biology of the Cell

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The location of the nucleus in statocytes or lentil roots grown: 1), at 1 g on the ground, 2), on a 1 g centrifuge in space, 3), in simulated microgravity on a slowly rotating clinostat (0.9 rmp) 4), in microgravity in space was investigated and statistically evaluated. In cells differentiated at 1 g on the ground, the nuclear membrane was almost in contact with the plasmalemma lining the proximal cell wall, whereas in statocytes of roots crown on the clinostat there was a distance of 0.47 micrometers (horizontal clinorotation) and or 0.76 micrometers (vertical clinorotation) between these membranes. However, in microgravity the nucleus was the most displaced, 0.87 micrometers from the proximal cell wall. Centrifugation of vertically grown roots in the root-tip direction showed that the threshold of centrifugal force to detach all nuclei from the proximal cell wall was about 40 g. In statocytes developed in the presence of cytochalasin B at 1 g the nuclei were sedimented on the amyloplasts at the distal cell pole, demonstrating that the location of the nucleus depends on actin filaments. The results obtained are in agreement with the hypothesis that gravity causes a tension of actin filaments and that this part of the cytoskeleton undergoes a relaxation in microgravity.

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Section: Discussionmentioning

confidence: 99%

Actin filaments responsible for the location of the nucleus in the lentil statocyte are sensitive to gravity

Lorenzi

Perbal

1990

Biology of the Cell

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“…These actin bundles are large, containing 50 to 100 microfilaments (Nagai and Rebhun, 1966) of uniform polarity (Palevitz and Hepler, 1975) in ordered, paracrystalline arrays (Nagai and Hayama, 1979). The bundles are also highly stable being resistant to breakdown during treatment with cytochalasin or during fixation with glutaraldehyde (Williamson, 1978), processes that disrupt actin filaments in other plants (Parthasarathy et al, 1985). The organisation and maintenance of actin bundles presumably requires the presence of one or several actin-bundling proteins.…”

Section: Introductionmentioning

confidence: 99%

Elongation factor 1α is a component of the subcortical actin bundles of characean algae.

Collings

Wasteneys

Miyazaki

et al. 1994

Cell Biology International

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Antibodies to elongation factor 1 alpha (EF1 alpha), a known 50 kDa actin-bundling protein in Dictyostelium, identified a protein in a whole cell extract of the characean alga Nitella pseudoflabellata that had an apparent molecular weight of 51 kDa. Indirect immunofluorescence microscopy revealed labelling by the EF1 alpha antibodies of the subcortical actin bundles, even after the motile organelles of the endoplasm were removed by perfusion with ATP-containing solutions.

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“…Because MFs are colocalized with MTs in certain cases, and cytochalasin D (CD) interferes with the maturation of some MT arrays [Menzel and Schliwa, 1986;Kakimoto and Shibaoka, 1987;Lloyd et al, 1987;Palevitz, 1987a,b;Uyeda and Furuya, 1987;Traas et al, 1987;Kobayashi 0 1991 Wiley-Liss, Inc. Menzel and Elsner-Menzel, 1989;Pierson et al, 1989;Serlin and Ferrell, 1989;Traas et al, 19891, organizational interactions between the two seem likely. Recently, based on the presence of associated MTs during cytokinesis, it has been proposed that the nuclear envelope (NE) serves as the initiation site for the interphase array [Clayton et al, 1985;Wick, 1985al (MFs are also associated with the NE during the cell cycle [e.g., Parthasarathy et al, 1985;Palevitz, 1987a; 7-day-old cell suspension were transferred to 95 ml of fresh medium and the suspension was then incubated for 24 hr with the addition of 5 pg/ml aphidicolin (Sigma Chemical Co., St. Louis, MO). The cells were then collected by sedimentation, washed with 10 volumes of fresh medium using a glass filter, and resuspended and cultured further in fresh medium.…”

Section: Introductionmentioning

confidence: 99%

Microtubule reorganization during the cell cycle in synchronized BY‐2 tobacco suspensions

Hasezawa

Marc

Palevitz

1991

Cell Motil. Cytoskeleton

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Tobacco BY-2 suspension cultures were synchronized with aphidicolin in order to assess the relationship between microtubules (MTs), microfilaments (MFs), and the nuclear envelope (NE) at different stages of the cell cycle. Using immunofluorescence techniques, ordered MT arrays were found in the cortex in G,; few MTs are evident deeper in the cytoplasm or near the nucleus. However, MTs radiate from the surface of the nucleus during S and G, as the interphase cortical array is replaced by the preprophase band. Pennuclear fluorescence is also visible at the end of cytokinesis but does not overlap with new ordered cortical arrays early in G,. When isolated nuclei are examined, associated MTs are again evident in S and G,, but not in G,. Microfilaments are colocalized with the MTs in the radiating arrays, as ascertained by dual staining of cells with rhodamine phalloidin. Propyzamide treatment leads to the loss of MTs at all stages, while cytoplasmic and perinuclear MF networks persist. Conversely, cytochalasin D disrupts MFs, including those radiating from the nucleus durmg S and G2, without any apparent effect on MTs. The results cast doubt on a proposed role for the NE in the generation of cortical MTs in plants. A universal role for MFs in the deployment of MTs is also in question.

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Actin Network as a Normal Component of the Cytoskeleton in Many Vascular Plant Cells

Cited by 107 publications

References 21 publications

Actin filaments responsible for the location of the nucleus in the lentil statocyte are sensitive to gravity

Actin filaments responsible for the location of the nucleus in the lentil statocyte are sensitive to gravity

Elongation factor 1α is a component of the subcortical actin bundles of characean algae.

Microtubule reorganization during the cell cycle in synchronized BY‐2 tobacco suspensions

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