Actin-binding protein anillin promotes the progression of hepatocellular carcinoma <i>in vitro</i> and in mice

Jia, Honglin; Gao, Zhongming; Fang, Yu; Guo, Hongwei; Li, Baoyu

doi:10.3892/etm.2021.9885

Cited by 6 publications

(5 citation statements)

References 32 publications

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“…For HCC, consistent with these findings, our previous study has already verified that Anillin was over-expressed in HCC and facilitated both tumor cell proliferation and tumor growth [35]. Similarly, at about the same time, our findings are also matched with some of the other authors' discoveries on the general over-expression of Anillin in HCC and its promoting effects on either tumorigenesis or development [36,37].…”

Section: Discussionsupporting

confidence: 91%

Raising of Anillin expression in para-cancerous hepatocytes is associated with hepatic depolyploidization and short-term recurrence of hepatocellular carcinoma after radical operation

Wang¹,

Hao²,

Zhang³

et al. 2022

J. Cancer

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Background: Short-term recurrence of hepatocellular carcinoma (HCC) after radical operation results in poor prognosis and short overall survival period. Assessment of the post-operational recurrence risk could provide an advantage for preventing lethal progress by assisting to set up necessarily individual adjuvant treatment. Here, on basis of our previous research on the ploidy status of hepatocytes, we detected the expression of Anillin, a pivotal regulating gene of depolyploidization in para-cancerous hepatocytes, and discussed the relation between its alternative expression and short-term recurrence of HCC patients after radical operation. Methods: One hundred and twenty-one specimens of the para-cancerous tissues from a cohort of HCC patients were collected. The RT-qPCR assay and the Immunohistochemistry assay were conducted for a thorough profile of Anillin expression, along with the analysis of patients' information from the GEO database. The clinicopathological para-maters of the patients were detected to determine the relationship between Anillin in the para--cancerous and the tumor relapse. Specimens treated with HE staining were examined for the precise count of the binuclear polyploid hepatocytes, and the karyoplasmic ratio was calculated. Results: Anillin was verified raised in the para-cancerous tissues of the patients who relapsed. The raising of Anillin in para-cancerous tissue is related to poor clinicopathologic features of HCC patients and short-term recurrence. Binuclear polyploid hepatocytes were reduced along with a higher expression of Anillin, and the proportion of high karyoplasmic ratio hepatocytes was significantly decreased. Conclusion:The raising of Anillin is associated with the depolyploidization of hepatocytes in the representation of losing binuclear hepatocytes and reducing proportion reduction of high karyoplasmic ratio hepatocytes. Measuring Anillin in para-cancerous tissue, along with the pathomorphological examination may provide us a strategy for screening high risk of HCC recurrence, and help to design individual adjuvant treatment post-operation.

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Section: Discussionsupporting

confidence: 91%

Raising of Anillin expression in para-cancerous hepatocytes is associated with hepatic depolyploidization and short-term recurrence of hepatocellular carcinoma after radical operation

Wang¹,

Hao²,

Zhang³

et al. 2022

J. Cancer

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“…However, numerous recent studies suggest that anillin is involved in tumorigenesis in various types of cells. For example [ 66 ], demonstrated that anillin is overexpressed in human gastric cancer (GC) tissues and that depletion of anillin in these tissues inhibits the proliferation of GC cells. Additionally, a recent study by Xiao and colleagues (2020) investigated the regulation mechanism of anillin in human hepatocellular carcinoma (HCC).…”

Section: Cytokinesis Anillin and Cancermentioning

confidence: 99%

The role of anillin/Mid1p during medial division and cytokinesis: from fission yeast to cancer cells

et al. 2022

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Cytokinesis is the final stage of cell division cycle when cellular constituents are separated to produce two daughter cells. This process is driven by the formation and constriction of a contractile ring. Progression of these events is controlled by mechanisms and proteins that are evolutionary conserved in eukaryotes from fungi to humans. Genetic and molecular studies in different model organisms identified essential cytokinesis genes, with several conserved proteins, including the anillin/Mid1p proteins, constituting the core cytokinetic machinery. The fission yeast Schizosaccharomyces pombe represents a well-established model organism to study eukaryotic cell cycle regulation. Cytokinesis in fission yeast and mammalian cells depends on the placement, assembly, maturation, and constriction of a medially located actin-myosin contractile ring (ACR). Here, we review aspects of the ACR assembly and cytokinesis process in fission yeast and consider the regulation of such events in mammalian cells. First, we briefly describe the role of anillin during mammalian ACR assembly and cytokinesis. Second, we describe different aspects of the anillin-like protein Mid1p regulation during the S. pombe cell cycle, including its structure, function, and phospho-regulation. Third, we briefly discuss Mid1pindependent ACR assembly in S. pombe. Fourth, we highlight emerging studies demonstrating the roles of anillin in human tumourigenesis introducing anillin as a potential drug target for cancer treatment. Collectively, we provide an overview of the current understanding of medial division and cytokinesis in S. pombe and suggest the implications of these observations in other eukaryotic organisms, including humans.

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“…In future, SIM-TIRF technology will be extended to the imaging of biological tissues with more complex structures and morphologies, such as macromolecular yeast nuclear-pore complexes [165,166]. Although theoretical research on the yeast nuclear-pore complex has reached an advanced stage, experimental verification remains challenging [167][168][169]. Another meaningful research area in the future is the SIM-cTIRF.…”

Section: Summary and Prospectsmentioning

confidence: 99%

Recent Progress in the Correlative Structured Illumination Microscopy

et al. 2021

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The super-resolution imaging technique of structured illumination microscopy (SIM) enables the mixing of high-frequency information into the optical transmission domain via light-source modulation, thus breaking the optical diffraction limit. Correlative SIM, which combines other techniques with SIM, offers more versatility or higher imaging resolution than traditional SIM. In this review, we first briefly introduce the imaging mechanism and development trends of conventional SIM. Then, the principles and recent developments of correlative SIM techniques are reviewed. Finally, the future development directions of SIM and its correlative microscopies are presented.

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Actin-binding protein anillin promotes the progression of hepatocellular carcinoma in vitro and in mice

Cited by 6 publications

References 32 publications

Raising of Anillin expression in para-cancerous hepatocytes is associated with hepatic depolyploidization and short-term recurrence of hepatocellular carcinoma after radical operation

Raising of Anillin expression in para-cancerous hepatocytes is associated with hepatic depolyploidization and short-term recurrence of hepatocellular carcinoma after radical operation

The role of anillin/Mid1p during medial division and cytokinesis: from fission yeast to cancer cells

Recent Progress in the Correlative Structured Illumination Microscopy

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