Actin-associated hnRNP proteins as transacting factors in the control of mRNA transport and localization

Percipalle, Piergiorgio; Raju, Chandrasekhar S.; Fukuda, Natsuki

doi:10.4161/rna.6.2.8195

Cited by 34 publications

(35 citation statements)

References 23 publications

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“…This study provides a characterization of RALY, a protein that belongs to the class of the RNA-binding proteins hnRNPs (55–59). RALY, previously known as hnRNP-C like-protein 2, shares common features with the majority of the hnRNPs: it contains a single RRM at the N-terminal region sharing 43% homology with hnRNP C, a glycine-rich region at the C-terminus and two uncharacterized NLS that have been predicted by computational analysis (27).…”

Section: Discussionmentioning

confidence: 99%

Identification and dynamic changes of RNAs isolated from RALY-containing ribonucleoprotein complexes

Rossi

Moro

Tebaldi

et al. 2017

Nucleic Acids Research

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RALY is a member of the heterogeneous nuclear ribonucleoprotein family (hnRNP), a large family of RNA-binding proteins involved in many aspects of RNA metabolism. Although RALY interactome has been recently characterized, a comprehensive global analysis of RALY-associated RNAs is lacking and the biological function of RALY remains elusive. Here, we performed RIP-seq analysis to identify RALY interacting RNAs and assessed the role of RALY in gene expression. We demonstrate that RALY binds specific coding and non-coding RNAs and associates with translating mRNAs of mammalian cells. Among the identified transcripts, we focused on ANXA1 and H1FX mRNAs, encoding for Annexin A1 and for the linker variant of the histone H1X, respectively. Both proteins are differentially expressed by proliferating cells and are considered as markers for tumorigenesis. We demonstrate that cells lacking RALY expression exhibit changes in the levels of H1FX and ANXA1 mRNAs and proteins in an opposite manner. We also provide evidence for a direct binding of RALY to the U-rich elements present within the 3΄UTR of both transcripts. Thus, our results identify RALY as a poly-U binding protein and as a regulator of H1FX and ANXA1 in mammalian cells.

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Section: Discussionmentioning

confidence: 99%

Identification and dynamic changes of RNAs isolated from RALY-containing ribonucleoprotein complexes

Rossi

Moro

Tebaldi

et al. 2017

Nucleic Acids Research

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“…In animals, localized RNAs are important determinants of cell fate in eggs and embryos, and such localization allows spatially restricted synthesis of specific proteins in distinct regions of the cytoplasm (21). During animal spermiogenesis, mRNA localization and translation are regulated in a stage-specific manner (22)(23)(24). During spermiogenesis, DNA-binding histones are replaced by protamines, resulting in chromatin condensation and cessation of transcription in elongating spermatids (25,26).…”

Section: A Combination Of Sequences In the 5′ Utr And Coding Region Arementioning

confidence: 99%

Intercellular communication in Arabidopsis thaliana pollen discovered via AHG3 transcript movement from the vegetative cell to sperm

Jiang

Boavida

et al. 2015

Proc. Natl. Acad. Sci. U.S.A.

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An Arabidopsis pollen grain (male gametophyte) consists of three cells: the vegetative cell, which forms the pollen tube, and two sperm cells enclosed within the vegetative cell. It is still unclear if there is intercellular communication between the vegetative cell and the sperm cells. Here we show that ABA-hypersensitive germination3 (AHG3), encoding a protein phosphatase, is specifically transcribed in the vegetative cell but predominantly translated in sperm cells. We used a series of deletion constructs and promoter exchanges to document transport of AHG3 transcripts from the vegetative cell to sperm and showed that their transport requires sequences in both the 5′ UTR and the coding region. Thus, in addition its known role in transporting sperm during pollen tube growth, the vegetative cell also contributes transcripts to the sperm cells.

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“…In addition, nucleocytoplasmatic shuttling of hnRNP A2 is thought to be important for mRNA nuclear export and localized translation (37). Therefore, we examined whether aldosterone modifies the steady-state subcellular localization of hnRNP A2/B1 in colon epithelia using cell fractionation.…”

Section: Resultsmentioning

confidence: 99%

Heterogeneous nuclear ribonucleoprotein A2/B1 is a tissue-specific aldosterone target gene with prominent induction in the rat distal colon

Giráldez

Morales

Hernández

et al. 2013

American Journal of Physiology-Gastrointestinal and Liver Physi

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The steroid hormone aldosterone enhances transepithelial Na(+) reabsorption in tight epithelia and is crucial to achieve extracellular volume homeostasis and control of blood pressure. One of the main transport pathways regulated by aldosterone involves the epithelial Na(+) channel (ENaC), which constitutes the rate-limiting step of Na(+) reabsorption in parts of the distal nephron and the collecting duct, the distal colon, and sweat and salivary glands. Although these epithelial tissues share the same receptor for aldosterone (mineralocorticoid receptor, MR), and the same transport system (ENaC), it has become clear that the molecular mechanisms involved in the modulation of channel activity are tissue-specific. Recent evidence suggests that aldosterone controls transcription and also translation of ENaC subunits in some cell types. A possible pathway for translational regulation is binding of regulatory proteins to ENaC subunit mRNAs, such as the heterogeneous nuclear ribonucleoprotein A2/B1 (hnRNP A2/B1). In this study, we examined whether hnRNP A2/B1 is an aldosterone-target gene in vivo. Our data show that physiological levels of aldosterone markedly induce hnRNP A2/B1 expression in an early and sustained manner in the late distal colon epithelium but not in other aldosterone-target tissues. The effect depends on MR but not on glucocorticoid receptor activity. We also demonstrate that the genomic region upstream of hnRNP A2/B1 contains aldosterone-responsive elements involved in the control of gene expression. We hypothesize that hnRNP A2/B1 is involved in the tissue-specific regulation of ENaC biosynthesis and may coordinate the response of other genes relevant for transepithelial Na(+) reabsorption by aldosterone.

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Actin-associated hnRNP proteins as transacting factors in the control of mRNA transport and localization

Cited by 34 publications

References 23 publications

Identification and dynamic changes of RNAs isolated from RALY-containing ribonucleoprotein complexes

Identification and dynamic changes of RNAs isolated from RALY-containing ribonucleoprotein complexes

Intercellular communication in Arabidopsis thaliana pollen discovered via AHG3 transcript movement from the vegetative cell to sperm

Heterogeneous nuclear ribonucleoprotein A2/B1 is a tissue-specific aldosterone target gene with prominent induction in the rat distal colon

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