Intercellular communication in
            <i>Arabidopsis thaliana</i>
            pollen discovered via
            <i>AHG3</i>
            transcript movement from the vegetative cell to sperm

Jiang, Hua; Yi, Jun; Boavida, Leonor C.; Chen, Yuan; Becker, Jörg; Köhler, Claudia; McCormick, Sheila

doi:10.1073/pnas.1510854112

Cited by 21 publications

(15 citation statements)

References 45 publications

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“…The authors provided evidence that AHG3 transcripts move from the vegetative cell to sperm cells, and that transport requires sequences in both the 5′ UTR and coding region. The mechanisms involved and the extent of communication via this route are not yet clear, but these results document an additional role for the vegetative cell in shaping the sperm cell transcriptome (Jiang et al, 2015). He et al 2015).…”

Section: Communication Between Non-germ and Germ Cellsmentioning

confidence: 97%

“…While studying the expression of the sperm cell-enriched ABA-related protein phosphatase gene ABA-HYPERSENSITIVE GERMINATION3 (AHG3), Jiang and coworkers (2015) unexpectedly found that the AHG3 promoter was transcriptionally active in the vegetative cell, whereas a translational fusion protein was localized to sperm (Jiang et al, 2015). The authors provided evidence that AHG3 transcripts move from the vegetative cell to sperm cells, and that transport requires sequences in both the 5′ UTR and coding region.…”

Section: Communication Between Non-germ and Germ Cellsmentioning

confidence: 99%

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The evolution and patterning of male gametophyte development

Hackenberg

Twell

2019

Current Topics in Developmental Biology

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1. The evolutionary history of the male gametophyte 1.1. The origins of spermatogenesis in green plants 1.2. The sporophyte was key for terrestrial plant evolution 1.3. Heterospory and the origin of microgametophytes 1.4. Adaptations of seed plant microgametophytes 2. Male germline development in angiosperms 2.1. Asymmetric division and cell fate determination 2.2. Cell cycle control and male gametophyte patterning 2.3. Transcriptional control of male gametophyte patterning 2.4. Chromatin remodelling in male gametophyte development 2.5. Communication between non-germ and germ cells 3. Molecular evolution of the male germline 3.1. Motile plant and animal sperm share common features 3.2. Gene expression in male germ cells 3.3. The evolution of transcription factors regulating male gametogenesis 4. Perspective 5. Acknowledgements 2 6. References Abstract: The reproductive adaptations of land plants have played a key role in their terrestrial colonization and radiation. These encompass mechanisms used for the production, dispersal and union of gametes to support sexual reproduction. The production of small motile male gametes and larger immotile female gametes (oogamy) in specialized multicellular gametangia evolved in the charophyte algae, the closest extant relatives of land plants.Reliance on water and motile male gametes for sexual reproduction was retained by bryophytes and basal vascular plants, but was overcome in seed plants by the dispersal of pollen and the guided delivery of non-motile sperm to the female gametes. Here we discuss the evolutionary history of male gametogenesis in streptophytes (green plants) and the underlying developmental biology, including recent advances in bryophyte and angiosperm models. We conclude with a perspective on research trends that promise to deliver a deeper understanding of the evolutionary and developmental mechanisms of male gametogenesis in plants.

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Section: Communication Between Non-germ and Germ Cellsmentioning

confidence: 97%

Section: Communication Between Non-germ and Germ Cellsmentioning

confidence: 99%

The evolution and patterning of male gametophyte development

Hackenberg

Twell

2019

Current Topics in Developmental Biology

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“…To generate atx4 atx5 double mutants, atx4-1 was crossed with atx5-1 mutants, and double mutants were screened using ATX4-1LP/RP and ATX5-1LP/RP PCR primers. To generate atx4-1 ahg3-1 and atx5-1 ahg3-1 double mutants and the atx4-1 atx5-1 ahg3-1 triple mutant, atx4-1, atx5-1 and atx4-1 atx5-1 mutants, respectively, were crossed with the ahg3-1 mutant (Nishimura et al, 2004;Jiang et al, 2015), and mutants were screened using primers ATX4-1LP/RP, ATX5-1LP/RP and AHG3-LP/RP, respectively. All PCR primers used for genotyping are listed in Supporting Information Table S1.…”

Section: Plant Growth Conditions and Genotypingmentioning

confidence: 99%

“…Thus, we reasoned that the hypersensitive phenotypes of the atx4 and atx5 mutants in response to ABA might be attributable to a reduction of AHG3 transcript levels in the mutants. To test this idea, we crossed the ahg3-1 mutant (Nishimura et al, 2004;Yoshida et al, 2006;Jiang et al, 2015) with the atx4-1, atx5-1 or atx4-1 atx5-1 mutant line and generated atx4-1 ahg3-1, atx5-1 ahg3-1, and atx4-1 atx5-1 ahg3-1 mutant lines, respectively, and further tested the ABA sensitivity in terms of seed germination. As shown in Fig.…”

Section: Atx4 and Atx5 Play Partially Redundant Roles In Plant Growthmentioning

confidence: 99%

Trithorax‐group proteins ARABIDOPSIS TRITHORAX4 (ATX4) and ATX5 function in abscisic acid and dehydration stress responses

et al. 2017

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Trithorax-group proteins (TrxGs) play essential regulatory roles in chromatin modification to activate transcription. Although TrxGs have been shown to be extensively involved in the activation of developmental genes, how the specific TrxGs function in the dehydration and abscisic acid (ABA)-mediated modulation of downstream gene expression remains unknown. Here, we report that two evolutionarily conserved Arabidopsis thaliana TrxGs, ARABIDOPSIS TRITHORAX4 (ATX4) and ATX5, play essential roles in the drought stress response. atx4 and atx5 single loss-of-function mutants showed drought stress-tolerant and ABA-hypersensitive phenotypes during seed germination and seedling development, while the atx4 atx5 double mutant displayed further exacerbation of the phenotypes. Genome-wide RNA-sequencing analyses showed that ATX4 and ATX5 regulate the expression of genes functioning in dehydration stress. Intriguingly, ABA-HYPERSENSITIVE GERMINATION 3 (AHG3), an essential negative regulator of ABA signaling, acts genetically downstream of ATX4 and ATX5 in response to ABA. ATX4 and ATX5 directly bind to the AHG3 locus and trimethylate histone H3 of Lys 4 (H3K4). Moreover, ATX4 and ATX5 occupancies at AHG3 are dramatically increased under ABA treatment, and are also essential for RNA polymerase II (RNAPII) occupancies. Our findings reveal novel molecular functions of A. thaliana TrxGs in dehydration stress and ABA responses.

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“…This physical connection allows it to travel as a unit through the growing pollen tube to the female gametophyte where fertilization of the egg and the central cell generates the embryo and the endosperm, respectively (McCue et al 2011 ; Ge et al 2011 ). Additionally, molecular data suggest that MGU linkage is essential for communication between the VC and SCs (Tian et al 1998 ; Slotkin et al 2009 ; Hamamura et al 2011 ; Grant-Downton et al 2013 ; Jiang et al 2015 ).…”

Section: Introductionmentioning

confidence: 99%

Epigenetic marks in the Hyacinthus orientalis L. mature pollen grain and during in vitro pollen tube growth

et al. 2016

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During the sexual reproduction of flowering plants, epigenetic control of gene expression and genome integrity by DNA methylation and histone modifications plays an important role in male gametogenesis. In this study, we compared the chromatin modification patterns of the generative, sperm cells and vegetative nuclei during Hyacinthus orientalis male gametophyte development. Changes in the spatial and temporal distribution of 5-methylcytosine, acetylated histone H4 and histone deacetylase indicated potential differences in the specific epigenetic state of all analysed cells, in both the mature cellular pollen grains and the in vitro growing pollen tubes. Interestingly, we observed unique localization of chromatin modifications in the area of the generative and the vegetative nuclei located near each other in the male germ unit, indicating the precise mechanisms of gene expression regulation in this region. We discuss the differences in the patterns of the epigenetic marks along with our previous reports of nuclear metabolism and changes in chromatin organization and activity in hyacinth male gametophyte cells. We also propose that this epigenetic status of the analysed nuclei is related to the different acquired fates and biological functions of these cells.Electronic supplementary materialThe online version of this article (doi:10.1007/s00497-016-0289-3) contains supplementary material, which is available to authorized users.

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Intercellular communication in Arabidopsis thaliana pollen discovered via AHG3 transcript movement from the vegetative cell to sperm

Cited by 21 publications

References 45 publications

The evolution and patterning of male gametophyte development

The evolution and patterning of male gametophyte development

Trithorax‐group proteins ARABIDOPSIS TRITHORAX4 (ATX4) and ATX5 function in abscisic acid and dehydration stress responses

Epigenetic marks in the Hyacinthus orientalis L. mature pollen grain and during in vitro pollen tube growth

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