Actin and dynamin2 dynamics and interplay during clathrin-mediated endocytosis

Grassart, Alexandre; Cheng, Aaron T.; Hong, Sun Hae; Zhang, Fan; Zenzer, Nathan; Feng, Yongmei; Briner, David M.; Davis, Gregory D.; Malkov, Dmitry; Drubin, David G.

doi:10.1083/jcb.201403041

Cited by 201 publications

(240 citation statements)

References 49 publications

(105 reference statements)

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“…As a control we evaluated the chance of random colocalization by shifting the position of the same image and found 4.6% colocalization. Previously, clathrin was shown to appear at CCS in a short-lived (< 20 s) and a long-lived population (> 20 s), proposed to represent abortive and productive events, respectively (Grassart et al, 2014; Loerke et al, 2009). We therefore monitored GFP-CK1δ and clathrin light chain-RFP simultaneously for 4 min and found that GFP-CK1δ was detected at 15.9% of short-lived CCS and at 32.7% of long-lived CCS (Fig.…”

Section: Resultsmentioning

confidence: 99%

Casein Kinase 1 Promotes Initiation of Clathrin-Mediated Endocytosis

Peng

Grassart

et al. 2015

Developmental Cell

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Summary In budding yeast, over 60 proteins functioning in at least 5 modules are recruited to endocytic sites with predictable order and timing. However, how sites of clathrin-mediated endocytosis are initiated and stabilized is not well understood. Here, the casein kinase 1 (CK1) Hrr25 is shown to be an endocytic protein and to be among the earliest proteins to appear at endocytic sites. Hrr25 absence or overexpression decreases or increases the rate of endocytic site initiation, respectively. Ede1, an early endocytic Eps15-like protein important for endocytic initiation, is an Hrr25 target and is required for Hrr25 recruitment to endocytic sites. Hrr25 phosphorylation of Ede1 is required for Hrr25-Ede1 interaction and promotes efficient initiation of endocytic sites. These observations indicate that Hrr25 kinase and Ede1 cooperate to initiate and stabilize endocytic sites. Analysis of the mammalian homologs CK1δ/ε suggests a conserved role for these protein kinases in endocytic site initiation and stabilization.

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Section: Resultsmentioning

confidence: 99%

Casein Kinase 1 Promotes Initiation of Clathrin-Mediated Endocytosis

Peng

Grassart

et al. 2015

Developmental Cell

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“…The shorter disorganized F-actin observed in dynamin 2 KO β cells is in line with a defect of dynamin-dependent actin elongation. Second, dynamin regulates actin reorganization through a mechanism downstream of the CME, where the complex actindynamin interactions occur (45,59,60,69). For instance, clathrin coats arrested on the PM are required for nucleating the actin regulators and polymerizing actin at CCPs (45).…”

Section: Discussionmentioning

confidence: 99%

Dynamin 2 regulates biphasic insulin secretion and plasma glucose homeostasis

Fan¹,

Chen²,

Wu³

et al. 2015

Journal of Clinical Investigation

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“…While the PH domain is dispensable for recruitment of dynamin to endocytic structures [76], its association with PI(4,5)P 2 is required for dynamin-mediated fission [55,56]. Dynamin oligomerizes into a helical assembly around the neck of constricted, late stage endocytic pits [73][74][75].…”

Section: Pi(45)p 2 In Endocytic Membrane Fissionmentioning

confidence: 99%