Acteoside ameliorates inflammatory responses through NFkB pathway in alcohol induced hepatic damage

Khullar, Mowkshi; Sharma, Ankita; Wani, Abubakar; Sharma, Neha; Sharma, Neelam; Chandan, B. K.; Kumar, Ajay; Ahmed, Zabeer

doi:10.1016/j.intimp.2019.01.020

Cited by 35 publications

(21 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Finally, caffeoyl CoA and hydroxytyrosol glucoside can be converted into acteoside by Shikimate O-hydroxycinnamoyltransferase (HCT) and UGT [18,25]. Recent studies have verified that acteoside possesses pharmacological properties: antioxidant, anti-inflammatory, antidepressant, antitumor, antidiabetes, and hepatoprotection [29,30,31,32].…”

Section: Introductionmentioning

confidence: 99%

Analysis of Centranthera grandiflora Benth Transcriptome Explores Genes of Catalpol, Acteoside and Azafrin Biosynthesis

Zhang

Wang

et al. 2019

IJMS

View full text Add to dashboard Cite

Cardiovascular diseases (CVDs) are a major cause of health loss in the world. Prevention and treatment of this disease by traditional Chinese medicine is a promising method. Centranthera grandiflora Benth is a high-value medicinal herb in the prevention and treatment of CVDs; its main medicinal components include iridoid glycosides, phenylethanoid glycosides, and azafrin in roots. However, biosynthetic pathways of these components and their regulatory mechanisms are unknown. Furthermore, there are no genomic resources of this herb. In this article, we provide sequence and transcript abundance data for the root, stem, and leaf transcriptome of C. grandiflora Benth obtained by the Illumina Hiseq2000. More than 438 million clean reads were obtained from root, stem, and leaf libraries, which produced 153,198 unigenes. Based on databases annotation, a total of 557, 213, and 161 unigenes were annotated to catalpol, acteoside, and azafrin biosynthetic pathways, respectively. Differentially expressed gene analysis identified 14,875 unigenes differentially enriched between leaf and root with 8,054 upregulated genes and 6,821 downregulated genes. Candidate MYB transcription factors involved in catalpol, acteoside, and azafrin biosynthesis were also predicated. This work is the first transcriptome analysis in C. grandiflora Benth which will aid the deciphering of biosynthesis pathways and regulatory mechanisms of active components.

show abstract

Section: Introductionmentioning

confidence: 99%

Analysis of Centranthera grandiflora Benth Transcriptome Explores Genes of Catalpol, Acteoside and Azafrin Biosynthesis

Zhang

Wang

et al. 2019

IJMS

View full text Add to dashboard Cite

show abstract

“…Pharmacodynamic interactions were found in binary mixtures using verbascoside as a leader compound. Verbascoside was selected as a leader compound because it is a major constitutive PSM in wild C. tenuiflora methanolic extracts and has demonstrated NF-κB/AP-1 inhibitory activity [4,[36][37][38]. Verbascoside-aucubin and verbascoside-kobusin mixtures showed synergism and verbascoside-tenuifloroside displayed a subadditivity effect in the inhibition of NF-κB/AP-1.…”

Section: Discussionmentioning

confidence: 99%

Synergism and Subadditivity of Verbascoside-Lignans and -Iridoids Binary Mixtures Isolated from Castilleja tenuiflora Benth. on NF-κB/AP-1 Inhibition Activity

et al. 2021

View full text Add to dashboard Cite

Pharmacodynamic interactions between plant isolated compounds are important to understand the mode of action of an herbal extract to formulate or create better standardized extracts, phytomedicines, or phytopharmaceuticals. In this work, we propose binary mixtures using a leader compound to found pharmacodynamic interactions in inhibition of the NF-κB/AP-1 pathway using RAW-Blue™ cells. Eight compounds were isolated from Castilleja tenuiflora, four were new furofuran-type lignans for the species magnolin, eudesmin, sesamin, and kobusin. Magnolin (60.97%) was the most effective lignan inhibiting the NF-κB/AP-1 pathway, followed by eudesmin (56.82%), tenuifloroside (52.91%), sesamin (52.63%), and kobusin (45.45%). Verbascoside, a major compound contained in wild C. tenuiflora showed an inhibitory effect on NF-κB/AP-1. This polyphenol was chosen as a leader compound for binary mixtures. Verbacoside-aucubin and verbascoside-kobusin produced synergism, while verbascoside-tenuifloroside had subadditivity in all concentrations. Verbascoside-kobusin is a promising mixture to use on NF-κB/AP-1 related diseases and anti-inflammatory C. tenuiflora-based phytomedicines.

show abstract

“…The amount of flavonoids extracted from C. oppositifolia was 12.23 ± 0.03 mg/g dry weight (DW), showing a higher content compared to the amounts reported for the same species collected in Pakistan (9.9 and 7.3 mg/g DW for methanol/chloroform and aqueous extracts, respectively) [20]. The sum of the amounts of diosmetin (2), quercetin-O-glucoside (13), 6,8-di-C-β-glucopyranosylchrysin (14), myricetin-dihexoside (19), baicalin (21), and anisofolin A (20) accounted for more than 5% of the identified flavonoids (Table 2), and among these, (20) was the most abundant flavonoid derivative in the extract, with an amount of 1.87 mg/g. Considering phenolic acids, the total amount in COME was 32.33 ± 0.51 mg/g DW, and again the result was in agreement with the 32.3 and 25.9 mg/g DW previously reported in methanol/chloroform and aqueous extracts of the same plant collected in Pakistan [20].…”

Section: Lc-ms Phytochemical Characterization Of C Oppositifolia Metmentioning

confidence: 98%

“…Flavonoids such as apigenin, alnustin, mosloflavone [ 12 ], and flavonoid glycosides such as negletein-6- O -β- d -glucopyranoside and 5,7,2′-trihydroxyflavone-2′- O -β-d-glucopyranoside [ 7 ] have been identified in the aerial parts, together with the terpenes phytol [ 1 ] and (+)-14,15-dinor-9α-hydroxy-cis-labd-11(E)-en-13-one [ 12 ]. C. oppositifolia is also a source of acteoside [ 13 ], a phenylethanoid glycoside that possess antioxidant, antimicrobial, neuroprotective, antitumor, and analgesic properties, and has been studied for its anti-inflammatory activity [ 14 ]. Nevertheless, studies on the anti-inflammatory potential of the plant are still lacking, despite its traditional uses.…”

Section: Introductionmentioning

confidence: 99%

Comprehensive Characterization of Secondary Metabolites from Colebrookea oppositifolia (Smith) Leaves from Nepal and Assessment of Cytotoxic Effect and Anti-Nf-κB and AP-1 Activities In Vitro

Peron

Hošek

Phuyal

et al. 2020

IJMS

View full text Add to dashboard Cite

Here we report the comprehensive characterization of the secondary metabolites from the leaves of Colebrookea oppositifolia Smith, a species used as medicinal plant in the traditional medicine of Nepal. Phytochemical screening of bioactives was performed using an integrated LC-MSn and high resolution MS (Mass Spectrometry) approach. Forty-three compounds were tentatively identified, mainly aglyconic and glycosilated flavonoids and phenolic acids, as well as other bioactives such as coumarins and terpenes were detected. Furthermore, the NF-κB and AP-1 inhibitory activity of C. oppositifolia extract were evaluated, as well as its cytotoxicity against THP-1 cells, in order to assess the potential use of this herb as a source of anti-inflammatory and cytotoxic compounds. The results so far obtained indicate that C. oppositifolia leaves extract could significantly reduce the viability of THP-1 cells (IC50 = 6.2 ± 1.2 µg/mL), as well as the activation of both NF-κB and AP-1 at the concentration of 2 μg/mL. Our results indicate that Nepalese C. oppositifolia is a valuable source of anti-inflammatory and cytotoxic compounds. The phytochemical composition reported here can partially justify the traditional uses of C. oppositifolia in Nepal, especially in the treatment of inflammatory diseases, although further research will be needed to assess the full potential of this species.

show abstract

Acteoside ameliorates inflammatory responses through NFkB pathway in alcohol induced hepatic damage

Cited by 35 publications

References 28 publications

Analysis of Centranthera grandiflora Benth Transcriptome Explores Genes of Catalpol, Acteoside and Azafrin Biosynthesis

Analysis of Centranthera grandiflora Benth Transcriptome Explores Genes of Catalpol, Acteoside and Azafrin Biosynthesis

Synergism and Subadditivity of Verbascoside-Lignans and -Iridoids Binary Mixtures Isolated from Castilleja tenuiflora Benth. on NF-κB/AP-1 Inhibition Activity

Comprehensive Characterization of Secondary Metabolites from Colebrookea oppositifolia (Smith) Leaves from Nepal and Assessment of Cytotoxic Effect and Anti-Nf-κB and AP-1 Activities In Vitro

Contact Info

Product

Resources

About