Acrylonitrile-based copolymers: Synthesis, characterization, and formation of ultrafiltration membranes utilized for the immobilization of proteins

Abstract

SynopsisVarious high molecular weight copolymers of acrylonitrile and a vinyl comonomer containing an aryl amine, a pyridine, or a n aliphatic hydroxyl group were synthesized via slurry polymerization techniques so as to contain from 1 to 15 mol % functional comonomer. The comonomer content was quantitated by ultraviolet absorbance, base titration of acid polymer salts, and/or relative chemical reactivity with trichloro-s-triazine. Thin films were cast from copolymer solutions, coagulated into unsupported ultrafiltration membranes, and characterized with respect to both water permeability and pore size distribution. Analysis by size exclusion chromatography of the membrane permeate of a pool of dextran fractions yielded a continuous distribution curve for membrane pore size over the range 1.5 to 70 nm. The ultrafiltration membranes were used for protein immobilization after appropriate chemical activation. The three distinct types of functional copolymers gave comparable results for achymotrypsin, with protein weight loadings of 6-12% and 4045% retention of enzymatic specific activity.

Synthetic oligodeoxynucleotide purification by capping failure sequences with a methacrylamide phosphoramidite followed by polymerization

“…14) and 18, 15 respectively using the same procedures for preparing 11. The intermediate compound 17 was known, 16 and 19 is new and was fully characterized.…”

Covalent Immobilization of Glucose Oxidase onto New Modified Acrylonitrile Copolymer/Silica Gel Hybrid Supports

“…Polyacrylonitrile (PAN) has a good film forming property and good mechanical properties, but it has not active groups for enzyme immobilization 27. The chemically modified PAN and copolymers of acrylonitrile were very suitable for this purpose 28–32. Many references can be obtained in literature about the immobilization of enzymes on membranes and beads prepared from chemically modified AN copolymer, but there is no information for using AN copolymer in preparing such hybrid support.…”

Incorporating α-Allyl Glucoside into Polyacrylonitrile by Water-Phase Precipitation Copolymerization To Reduce Protein Adsorption and Cell Adhesion

“…Copolymerizing hydrophilic monomers such as acrylic acid, acrylamides, vinylpyrrolidone, 2-hydroxyethyl methacrylate, glycidyl methacrylate, and 4-vinylpyridine with acrylonitrile have also been used to improve the properties of acrylonitrile-based polymeric membranes. 3,[5][6][7][8]11 However, much attention 3,[5][6]8 was focused on the pervaporation properties and enzyme immobilization for these copolymer membranes. Only fragmentary data 11 were reported concerning the improvement of fouling reducing.…”