Acromegaly Related to Tumoral Secretion of Growth Hormone-Releasing Factor

Assay of Growth Hormone Levels in Human Plasma using Commercial Kits: Analysis of Some Factors Influencing the Results

“…These forms have been observed 60 minutes after an intravenous bolus injection of GHRH (8,9). In the same studies, dramatic changes in the proportions of these plasma immunoreactive GH circulating forms were observed (8,9).…”

“…At least three major forms of circulating plasma GH have been described: the monomeric free form, containing the 22 kDa and the 20 kDa forms; a larger molecular weight immunoreactive GH of approximately 44 kDa, known as 'big' GH, corresponding to a GH dimer; 'big-big' immunoreactive GH, of a molecular weight of approximately 90 kDa, corresponding in part to GH bound to its binding protein (BP) (9)(10)(11)(12)(13). These forms have been observed 60 minutes after an intravenous bolus injection of GHRH (8,9). In the same studies, dramatic changes in the proportions of these plasma immunoreactive GH circulating forms were observed (8,9).…”