Assay of Growth Hormone Levels in Human Plasma using Commercial Kits: Analysis of Some Factors Influencing the Results

Chatelain, Pierre; Bouillat, B.; Cohen, Richard A.; Sassolas, G.; Souberbielle, Jean‐Claude; Ruitton, A; Joly, M; Job, J C

doi:10.1111/j.1651-2227.1990.tb11673.x

Cited by 33 publications

(21 citation statements)

References 14 publications

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“…Our results support the idea that the GH heterogeneity contributes to the discrepant results obtained when serum GH levels are measured by different assays (44)(45)(46). Isoforms and fragments lack part of the GH molecule, resulting in variable crossreactivity to monoclonal or polyclonal antibodies with distinct epitope specificity (47).…”

Section: Figuresupporting

confidence: 82%

Circulating non-22 kDa growth hormone isoforms in healthy children of normal stature: relation to height, body mass and pubertal development

Boguszewski

Jansson²,

Boguszewski³

et al. 1997

European Journal of Endocrinology

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The proportion of non-22 kDa GH isoforms was evaluated in 93 healthy children (48 boys aged 6.8-18.4 years and 45 girls aged 3.9-18.4 years) of normal stature (height Ϯ 2 S.D. score) at different stages of puberty. In addition, correlations among the proportion of non-22 kDa GH isoforms, auxology, spontaneous GH secretion and biochemical measurements were investigated. Serum non-22 kDa GH levels, expressed as percentage of total GH concentration in the samples, were determined by the 22 kDa GH exclusion assay, in which monomeric and dimeric 22 kDa GH are removed from serum and the non-22 kDa GH isoforms are quantitated using a polyclonal antibody GH assay. Samples were selected from spontaneous GH peaks in 24-h GH profiles. For boys, the median proportion of non-22 kDa GH isoforms was 8.5% (range 3.2-26.6%) and for girls it was 9.6% (1.8-17.4%), with no influence of age and no sex-related difference in prepubertal (boys, 7.2%; girls, 8.8%) or pubertal children (boys, 9.1%; girls, 9.9%). However, the median proportion of non-22 kDa GH isoforms was significantly higher in pubertal boys (9.1%) than in prepubertal boys (7.2%; P = 0.03). In pubertal boys, height S.D. scores (SDS) were inversely correlated to the proportion of non-22 kDa GH isoforms (r = ¹0.38; P = 0.02), especially at mid-puberty (r = ¹0.7; P = 0.01), indicating that the presence of increased amounts of circulating non-22 kDa GH isoforms was associated with less growth. In prepubertal children, positive correlations between non-22 kDa GH and weight SDS (r = 0.46; P = 0.03), weight-for-height SDS (r = 0.51; P = 0.01) and body mass index (r = 0.42; P = 0.04) were observed. No significant correlations were seen with spontaneous GH secretion or measurements of IGF-1, IGF-binding protein-3, insulin and leptin. These findings in normal children indicate that the proportion of circulating non-22 kDa GH isoforms may have physiologic significance for growth and metabolism in different stages of development, and emphasize the importance of evaluating the circulating ratio of 22 kDa and non-22 kDa GH in children with growth disorders.

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Section: Figuresupporting

confidence: 82%

Circulating non-22 kDa growth hormone isoforms in healthy children of normal stature: relation to height, body mass and pubertal development

Boguszewski

Jansson²,

Boguszewski³

et al. 1997

European Journal of Endocrinology

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“…In comparative studies, it has been demonstrated that great differences exist between laboratories using the same GH kit [17], These discrepancies have partly been attributed to local factors. In some countries, great effort is made to reveal these differences [18].…”

Section: 'Local Factors'mentioning

confidence: 99%

Factors Modifying Growth Hormone Estimates in Immunoassays

Fisker

Ørskov

1996

Horm Res

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Growth hormone (GH) in human serum has been measured for more than 30 years and during this period it has been used diagnostically in growth disturbances and GH disorders. The first GH determinations were by radioimmunoassays using polyclonal antibodies. Due to the low specificity of these assays and the variable molecular appearance of GH, higher levels were obtained than with later assays employing two highly specific monoclonal antibodies. Because of this variability between assays, empiric GH cutoff levels had to be developed in different laboratories. However, with the growing need for more sophisticated diagnosis, especially in adulthood GH deficiency, knowledge of factors influencing the individual GH assays is required. Since GH deficiency in adulthood is now treated with GH substitution therapy, the relevance of factors influencing GH estimates has become increasingly evident, because no specific symptoms exist in adult GH deficiency.

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“…As it is well known that GH concentrations vary according to the assay method [7][8][9][10][11], we have standardized the GH values measured with these commercial kits. The study described here was performed in 1996.…”

Section: Discussionmentioning

confidence: 99%

Registration System for Growth Hormone (GH) Treatment with Standardized Immunoreactive GH Values in Japan.

et al. 1998

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Assay of Growth Hormone Levels in Human Plasma using Commercial Kits: Analysis of Some Factors Influencing the Results

Cited by 33 publications

References 14 publications

Circulating non-22 kDa growth hormone isoforms in healthy children of normal stature: relation to height, body mass and pubertal development

Circulating non-22 kDa growth hormone isoforms in healthy children of normal stature: relation to height, body mass and pubertal development

Factors Modifying Growth Hormone Estimates in Immunoassays

Registration System for Growth Hormone (GH) Treatment with Standardized Immunoreactive GH Values in Japan.

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