Acquisition of plasmid-borne blaIMP-19 gene by a VIM-1-positive Pseudomonas aeruginosa of the sequence type 235 epidemic lineage

Pollini, Simona; Antonelli, Alberto; Venturelli, Claudia; Maradei, Simona; Veggetti, Alberto; Bracco, Silvia; Rumpianesi, Fabio; Luzzaro, Francesco; Rossolini, Gian María

doi:10.1093/jac/dks440

Cited by 11 publications

(5 citation statements)

References 9 publications

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“…The international clones ST111 (clonal complex CC111) and ST235 (CC235) were the most prevalent, comprising 26 and 21 of the 55 VIM producers genotyped, respectively (Table 1). Both STs have been reported previously among VIM producers recovered from the university hospital of Patras, South Greece [21], and from other European countries, such as Sweden, Germany, France, Italy, Spain, and Bulgaria [12,13,21-26]. …”

Section: Resultssupporting

confidence: 56%

Carbapenemase-producing Pseudomonas aeruginosafrom central Greece: molecular epidemiology and genetic analysis of class I integrons

et al. 2013

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BackgroundMultidrug-resistant Pseudomonas aeruginosa is a serious challenge for antimicrobial therapy of nosocomial infections, as it possesses several mechanisms of antimicrobial resistance. In Central Greece, a sudden increase of infections caused by carbapenem-resistant P. aeruginosa was observed during 2011, indicating the need for further analysis.MethodsFive-hundred and sixty-eight P. aeruginosa isolates were collected consecutively during an 8-month period in 2011 from inpatients treated in three hospitals in the Thessaly region (1,000,000 habitants) of Greece. Carbapenem-resistant P. aeruginosa (n = 284) were characterized by antimicrobial susceptibility testing and β-lactamase content, and the genetic relatedness of carbapenemase-producing isolates was assessed by BOX-PCR, multilocus sequence typing, and eBURST analysis. Mapping of the class I integrons of Verona integron-encoded metallo-β-lactamase (VIM)-carrying isolates was also performed, and clinical data of the VIM producers were reviewed.ResultsEighty (14.1%) out of the 568 P. aeruginosa isolates recovered from clinical specimens were VIM producers. Multilocus sequence typing revealed high prevalence of the international clones ST111 and ST235 among blaVIM-2- and blaVIM-4-positive isolates, respectively. blaVIM-17 was identified in an isolate of a novel sequence type (ST1457). blaVIM gene cassettes were carried by five distinct class I integrons, including two novel ones.ConclusionsSince the first report of VIM-producing P. aeruginosa in 2000, this microorganism still remains among the most prevalent multidrug resistant pathogens in Greece. The spread of VIM-producers belonging to the most common international clones (ST111 and ST235), the spread of integrons of divergent structures, and the emergence of novel integrons underscore their ongoing evolution.

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Section: Resultssupporting

confidence: 56%

Carbapenemase-producing Pseudomonas aeruginosafrom central Greece: molecular epidemiology and genetic analysis of class I integrons

et al. 2013

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“…The situation is aggravated by the emergence of MBL-producing and HiRiC strains. HiRiC isolates are known to carry MBL encoding genes and have been widely disseminated around the world ( 22 , 23 ). Here, we not only detected strains of six global HiRiCs STs but also found that 22.8% of the test strains harbored exogenous AMR genes.…”

Section: Discussionmentioning

confidence: 99%

Epidemiological and Genetic Characteristics of Clinical Carbapenem-Resistant Pseudomonas aeruginosa Strains in Guangdong Province, China

Zhao

Chen

et al. 2023

Microbiol Spectr

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Carbapenem-resistant Pseudomonas aeruginosa (CRPA) is a major concern in clinical settings worldwide, yet few genetic and epidemiological studies on CRPA strains have been performed in China. Here, we sequence and analyze the genomes of 416 P. aeruginosa strains from hospitals in China to elucidate the genetic, phenotypic, and transmission characteristics of CRPA strains and to identify the molecular signatures responsible for the observed increase in the prevalence of CRPA infections in China.

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“…The population structure of P. aeruginosa is described as non-clonal and epidemic [10]. Several high-risk clonal lineages including ST111, ST235 and ST175 carry genes that confer resistance to b-lactam antibiotics [10][11][12][13][14][15][16][17][18] and ST235 clones commonly found in Asia are predominantly associated with the dissemination of IMP type b-lactamase resistance genes [15][16][17][19][20][21][22]. MDR P. aeruginosa & 2016 The Authors.…”

Section: Introductionmentioning

confidence: 99%

Genomic islands 1 and 2 play key roles in the evolution of extensively drug-resistant ST235 isolates of Pseudomonas aeruginosa

et al. 2016

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Pseudomonas aeruginosa are noscomially acquired, opportunistic pathogens that pose a major threat to the health of burns patients and the immunocompromised. We sequenced the genomes of P. aeruginosa isolates RNS_PA1, RNS_PA46 and RNS_PAE05, which displayed resistance to almost all frontline antibiotics, including gentamicin, piperacillin, timentin, meropenem, ceftazidime and colistin. We provide evidence that the isolates are representatives of P. aeruginosa sequence type (ST) 235 and carry Tn6162 and Tn6163 in genomic islands 1 (GI1) and 2 (GI2), respectively. GI1 disrupts the endA gene at precisely the same chromosomal location as in P. aeruginosa strain VR-143/97, of unknown ST, creating an identical CA direct repeat. The class 1 integron associated with Tn6163 in GI2 carries a blaGES-5–aacA4–gcuE15–aphA15 cassette array conferring resistance to carbapenems and aminoglycosides. GI2 is flanked by a 12 nt direct repeat motif, abuts a tRNA-gly gene, and encodes proteins with putative roles in integration, conjugative transfer as well as integrative conjugative element-specific proteins. This suggests that GI2 may have evolved from a novel integrative conjugative element. Our data provide further support to the hypothesis that genomic islands play an important role in de novo evolution of multiple antibiotic resistance phenotypes in P. aeruginosa.

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Acquisition of plasmid-borne blaIMP-19 gene by a VIM-1-positive Pseudomonas aeruginosa of the sequence type 235 epidemic lineage

Cited by 11 publications

References 9 publications

Carbapenemase-producing Pseudomonas aeruginosafrom central Greece: molecular epidemiology and genetic analysis of class I integrons

Carbapenemase-producing Pseudomonas aeruginosafrom central Greece: molecular epidemiology and genetic analysis of class I integrons

Epidemiological and Genetic Characteristics of Clinical Carbapenem-Resistant Pseudomonas aeruginosa Strains in Guangdong Province, China

Genomic islands 1 and 2 play key roles in the evolution of extensively drug-resistant ST235 isolates of Pseudomonas aeruginosa

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