Acquisition of Host-Derived CD40L by HIV-1<i>In Vivo</i>and Its Functional Consequences in the B-Cell Compartment

Imbeault, Michaël; Ouellet, Michel; Giguère, Katia; Bertin, Jonathan; Bélanger, Danièle; Martin, Geneviève; Tremblay, Michel J.

doi:10.1128/jvi.01993-10

Cited by 50 publications

(48 citation statements)

References 56 publications

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“…On the other hand, CK6 has been ascribed to the CCL17/22 group. In mammals, CD40-stimulated B cells secrete CCL17 and CCL22, attracting memory CD4 + T cells to an antigen rich environment [50]. In this study, we have demonstrated the chemoattractant capacity of CK5B and CK6.…”

Section: Discussionmentioning

confidence: 66%

DNA vaccination against a fish rhabdovirus promotes an early chemokine-related recruitment of B cells to the muscle

Castro¹,

Martinez-Alonso²,

Fischer³

et al. 2014

Vaccine

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a b s t r a c tIn fish, intramuscular (i.m) injection of plasmid DNA encoding viral proteins has proved a highly effective vaccination strategy against some viral pathogens. The efficacy of DNA vaccination in teleost fish is based on the high level of viral antigen expression in muscle cells inducing a strong and long-lasting protection. However, the mechanisms through which this protection is established and effectuated in fish are still not fully understood. Moreover, similarities to mammalian models cannot be established since DNA vaccination in mammals usually induces much weaker responses. In this work, we have focused on the characterization of the immune cells that infiltrate the muscle at the site of DNA injection in vaccinated fish and the chemokines and chemokine receptors that may be involved in their infiltration. We have demonstrated through diverse techniques that B lymphocytes, both IgM + and IgT + cells, represented a major infiltrating cell type in fish vaccinated with a viral haemorrhagic septicaemia virus (VHSV) glycoprotein-encoding DNA vaccine, whereas in control fish injected with an oil adjuvant mainly granulocyte/monocyte-type cells were attracted. Among twelve chemokine genes studied, only CXCL11 L1, CK5B and CK6 mRNA levels were up-regulated in DNA vaccinated fish compared to fish injected with the corresponding vector backbone. Furthermore, the transcription of CXCR3B, a possible receptor for CXCL11 L1 was also significantly up-regulated in vaccinated fish. Finally, experiments performed with recombinant trout CK5B and CK6 and chemokine expression plasmids revealed that these chemokines have chemotactic capacities which might explain the recruitment of B cells to the site of DNA injection. Altogether, our results reveal that there is an early chemokine-related B cell recruitment triggered by i.m. DNA vaccination against VHSV which might play an important role in the initial phase of the immune response.

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Section: Discussionmentioning

confidence: 66%

DNA vaccination against a fish rhabdovirus promotes an early chemokine-related recruitment of B cells to the muscle

Castro¹,

Martinez-Alonso²,

Fischer³

et al. 2014

Vaccine

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“…It is worth mentioning in this respect that CD40 ligand (CD40L), a co-stimulatory molecule expressed by activated T lymphocytes, can be inserted at the surface of HIV-1 particles when budding from activated CD4+ T cells [23]. In particular, CD40L-bearing virions were shown to potently activate B lymphocytes, closely reproducing the functional consequences of the physiological activation of the natural CD40 ligand expressed by B cells [24,25]. As part of CD40L-triggered events, hyperactivated B cells express activation-induced cytidine deaminase (AICD), a DNA editing enzyme mediating immunoglobulin class switch recombination and somatic hypermutation [26].…”

Section: Hiv Infectionmentioning

confidence: 98%

Microenvironment and HIV-related lymphomagenesis

Pantanowitz¹,

Carbone²,

Dolcetti³

2015

Seminars in Cancer Biology

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“…Different cytokines and growth factors have been suggested to affect basic B cell functions because they are increased in the serum of HIV-1-infected individuals (14)(15)(16)(17)(18)(19). The B cell-activating factor (BAFF) (20,21) is one such cytokine that is mainly expressed by myeloid lineage cells (22,23) and is a potent regulator of B cell development and functions (24)(25)(26)(27).…”

Section: H Uman Immunodeficiency Virus 1 Infection Is Associatedmentioning

confidence: 99%

HIV-1–Triggered Release of Type I IFN by Plasmacytoid Dendritic Cells Induces BAFF Production in Monocytes

Gómez

Ouellet

Tremblay

2015

The Journal of Immunology

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HIV-1 infection leads to numerous B cell abnormalities, including hypergammaglobulinemia, nonspecific B cell activation, nonspecific class switching, increased cell turnover, breakage of tolerance, increased immature/transitional B cells, B cell malignancies, as well as a loss of capacity to generate and maintain memory, all of which contribute to a global impairment of the immune humoral compartment. Several cytokines and soluble factors, which are increased in sera of HIV-1–infected individuals, have been suggested to directly or indirectly contribute to these B cell dysfunctions, and one of these is the B cell–activating factor (BAFF). We report in this study that HIV-1 (X4- and R5-tropic) upregulates BAFF expression and secretion by human monocytes. Moreover, we show that the virus-mediated production of BAFF by monocytes relies on a type I IFN response by a small percentage of plasmacytoid dendritic cells (pDCs) present in the monocyte cultures. HIV-1–induced type I IFN by pDCs triggers BAFF production in both classical and intermediate monocytes, but not in nonclassical monocytes, which nonetheless display a very strong basal BAFF production. We report also that basal BAFF secretion was higher in monocytes obtained from females compared with those from male donors. This study provides a novel mechanistic explanation for the increased BAFF levels observed during HIV-1 infection and highlights the importance of pDC/monocyte crosstalk to drive BAFF secretion.

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Acquisition of Host-Derived CD40L by HIV-1In Vivoand Its Functional Consequences in the B-Cell Compartment

Cited by 50 publications

References 56 publications

DNA vaccination against a fish rhabdovirus promotes an early chemokine-related recruitment of B cells to the muscle

DNA vaccination against a fish rhabdovirus promotes an early chemokine-related recruitment of B cells to the muscle

Microenvironment and HIV-related lymphomagenesis

HIV-1–Triggered Release of Type I IFN by Plasmacytoid Dendritic Cells Induces BAFF Production in Monocytes

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