Acquired Tolerance to Oxidative Stress in Bifidobacterium longum 105-A via Expression of a Catalase Gene

He, Jianlong; Sakaguchi, Kouta; Suzuki, Tomohiko

doi:10.1128/aem.07093-11

Cited by 27 publications

(17 citation statements)

References 28 publications

(28 reference statements)

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“…Therefore, it is possible to postulate an association between the lipid oxidation phenomena and the previously reported reduced growth rate. Indeed, He et al 44 reported that the ability to cope with oxidative stress is a key factor in dramatically improving cell survival in bifidobacteria.…”

Section: Resultsmentioning

confidence: 99%

Linoleic acid induces metabolic stress in the intestinal microorganism Bifidobacterium breve DSM 20213

Senizza

Rocchetti

Callegari

et al. 2020

Sci Rep

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Despite clinical and research interest in the health implications of the conjugation of linoleic acid (LA) by bifidobacteria, the detailed metabolic pathway and physiological reasons underlying the process remain unclear. This research aimed to investigate, at the molecular level, how LA affects the metabolism of Bifidobacterium breve DSM 20213 as a model for the well-known LA conjugation phenotype of this species. the mechanisms involved and the meaning of the metabolic changes caused by LA to B. breve DSM 20213 are unclear due to the lack of comprehensive information regarding the responses of B. breve DSM 20213 under different environmental conditions. Therefore, for the first time, an untargeted metabolomics-based approach was used to depict the main changes in the metabolic profiles of B. breve DSM 20213. Both supervised and unsupervised statistical methods applied to the untargeted metabolomic data allowed confirming the metabolic changes of B. breve DSM 20213 when exposed to LA. In particular, alterations to the amino-acid, carbohydrate and fatty-acid biosynthetic pathways were observed at the stationary phase of growth curve. Among others, significant upregulation trends were detected for aromatic (such as tyrosine and tryptophan) and sulfur amino acids (i.e., methionine and cysteine). Besides confirming the conjugation of LA, metabolomics suggested a metabolic reprogramming during the whole growth curve and an imbalance in redox status following LA exposure. Such redox stress resulted in the down-accumulation of peroxide scavengers such as lowmolecular-weight thiols (glutathione-and mycothiol-related compounds) and ascorbate precursors, together with the up-accumulation of oxidized (hydroxy-and epoxy-derivatives) forms of fatty acids. Consistently, growth was reduced and the levels of the oxidative stress marker malondialdehyde were higher in LA-exposed B. breve DSM 20213 than in the control.

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Section: Resultsmentioning

confidence: 99%

Linoleic acid induces metabolic stress in the intestinal microorganism Bifidobacterium breve DSM 20213

Senizza

Rocchetti

Callegari

et al. 2020

Sci Rep

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“…For example, the proportion of gut obligate anaerobic genus Bifidobacterium and Clostridium showed no significant different between the feces and dust. Certain strains of anaerobic genus Clostridium can make endospore to survive in the aerobic condition and certain strains of Bifidobacterium acquired tolerance to oxidative stress [17,18]. Likewise, anaerobic microbes can survive in aerobic condition by adaptation using their own defense mechanisms and construct similar pattern.…”

Section: Genus (%) Cattle Horsementioning

confidence: 99%

Differences in microbiome and virome between cattle and horses in the same farm

Park

Kim

2020

Asian-Australas J Anim Sci

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Objective: The ecosystem of an animal farm is composed of various elements, such as animals, farmers, plants, feed, soil, and microorganisms. A domesticated animal’s health is largely connected with the reservoir of bacteria and viruses in animal farms. Although a few studies have focused on exploring the gut microbiome of animals, communities of microbiota and viruses in feedlots have not been thoroughly investigated.Methods: Here, we collected feces and dust samples (4 groups: cattle feces, C_F; horse feces, H_F; cattle dust, C_D; and horse dust, H_D) from cattle and horse farms sharing the same housing and investigated their microbiome/virome communities by Illumina sequencing.Results: Dust groups (C_D and H_D) showed higher microbial diversity than feces groups (C_F and H_F) regardless of animal species. From the microbial community analysis, all the samples from the four groups have major phyla such as Proteobacteria (min 37.1% to max 42.8%), Firmicutes (19.1% to 24.9%), Bacteroidetes (10.6% to 22.1%), and Actinobacteria (6.1% to 20.5%). The abundance of Streptococcus, which commonly recognized as equine pathogens, was significantly higher in the horse group (H_D and H_F). Over 99% among the classified virome reads were classified as Caudovirales, a group of tailed bacteriophages, in all four groups. Foot-and-mouth disease virus and equine adenovirus, which cause deadly diseases in cattle and horse, respectively, were not detected.Conclusion: Our results will provide baseline information to understand different gut and environmental microbial ecology between two livestock species.

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“…subtilis. Catalase has also been reported to protect bifidobacteria from oxidative stress (He et al, 2012). The global gene transcription and metabolic interactions in the mixed cultures are however not understood.…”

Section: Introductionmentioning

confidence: 99%

RNA-Seq reveals transcriptomic interactions of Bacillus subtilis natto and Bifidobacterium animalis subsp. lactis in whole soybean solid-state co-fermentation

Wang

Koh

et al. 2015

Food Microbiology

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Acquired Tolerance to Oxidative Stress in Bifidobacterium longum 105-A via Expression of a Catalase Gene

Cited by 27 publications

References 28 publications

Linoleic acid induces metabolic stress in the intestinal microorganism Bifidobacterium breve DSM 20213

Linoleic acid induces metabolic stress in the intestinal microorganism Bifidobacterium breve DSM 20213

Differences in microbiome and virome between cattle and horses in the same farm

RNA-Seq reveals transcriptomic interactions of Bacillus subtilis natto and Bifidobacterium animalis subsp. lactis in whole soybean solid-state co-fermentation

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