Acquired subglottic stenosis—depth and not extent of the insult is key

Dohar, Joseph E.; Klein, Edwin; Betsch, Jean L.; Hebda, Patricia A.

doi:10.1016/s0165-5876(98)00163-3

Cited by 58 publications

(98 citation statements)

References 27 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Many methods have been used to induce a model of airway stenosis that include long-term endotracheal intubation [2], laser cauterization [3], electrocautery [4][5][6], brushings [7], and the application of a cauterant such as hydrochloric acid [4], silver nitrate [4] or ethanol [6]. However, these methods are associated with a range of variables such as inaccurate and unpredictable results, complicated and difficult operations, and severe injury.…”

Section: Introductionmentioning

confidence: 99%

A model of canine tracheal stenosis induced by radiofrequency cauterization

Chen

Luo

et al. 2012

International Journal of Pediatric Otorhinolaryngology

View full text Add to dashboard Cite

Section: Introductionmentioning

confidence: 99%

A model of canine tracheal stenosis induced by radiofrequency cauterization

Chen

Luo

et al. 2012

International Journal of Pediatric Otorhinolaryngology

View full text Add to dashboard Cite

“…While these injuries provide predictable hisotologic changes, the ability to achieve a predictable overall degree of stenosis has not been clearly demonstrated. 8,11 Although still unable to obtain highly consistent degrees of stenosis, we still demonstrate an extremely rapid and reliable method to induce tracheal injury and resultant stenosis.…”

Section: Resultsmentioning

confidence: 87%

“…Depth of injury has been shown previously to be more closely correlated with the degree of tracheal stenosis. 8,13 The much greater degree of stenosis and higher mortality rate in the Nakagishi group are evidence that their depth of injury was greater.…”

Section: Resultsmentioning

confidence: 98%

“…The majority of methods to induce tracheal stenosis in animal models use an open technique in a variety of ways, including: scalpel, 2,5 curette, 6,7 hydrochloric acid, 8 silver nitrate, 8 electrocautery, 8 Bard Parker blade, 9 diode laser, 10 or carbon dioxide laser. 11 We chose to model our open group after a method described by Nakagishi et al 12 In their model, they produced an injury through a tracheotomy with a 5.5 mm nylon brush with a total of 10 brush strokes.…”

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

Tracheal Stenosis Induction: A Comparison of Open Versus Endoscopic Methods in a Rabbit Model

Wycherly¹,

Hesham²,

Malekzadeh³

et al. 2009

The Laryngoscope

View full text Add to dashboard Cite

EDUCATIONAL OBJECTIVE At the conclusion of this presentation, the participants should be able to appreciate the value of an endoscopic method of inducing tracheal stenosis in a rabbit model. OBJECTIVE METHODSFifteen adult male New Zealand White rabbits underwent induction of tracheal stenosis. Six animals received mucosal injury directly through a tracheotomy and 10 strokes of a nylon brush. Nine animals received tracheal injury endoscopically: a rigid bronchoscope was placed a measured distance below the inferior edge of the cricoid cartilage and a nylon brush was passed 4 times in each of the 4 quadrants. Endoscopies were performed 3 weeks after injury. Stenosis was measured as a percentage of luminal narrowing by a consensus of 3 individuals viewing the procedure on a television monitor. RESULTSOnly 1 animal in the tracheotomy group had noticeable stenosis (40%). In the endoscopic group, all animals had some degree of stenosis, ranging from 10-80% (mean 40%). The mortality rate was 20% (3/15). In the tracheotomy group, 1 mortality occurred secondary to antibiotic-induced gastroenteritis. In the endoscopic group, 2 mortalities occurred in the immediate postoperative period secondary to laryngeal edema. These 2 animals were the first to undergo the procedure, which lasted approximately 30 minutes. After refining our technique, succeeding procedures lasted an average of 5 minutes and there were no mortalities. The average duration of the tracheotomy procedure was 45 minutes. CONCLUSIONSUsing an endoscopic method can greatly improve the induction of tracheal stenosis by reducing operative time and eliminating an open wound and the need for antibiotics. It also achieves stenosis more consistently than an open technique.

show abstract

“…Moreover, substantial evidence indicates that the development of a keloid or hypertrophic scar is associated with abnormal fibroblast activity in the wound bed [2,3]. Thus, inhibiting proliferation of fibroblasts and inducing fibroblast apoptosis with pharmaceutical reagents may be a promising approach to treat airway restenosis.…”

Section: Introductionmentioning

confidence: 99%

Inhibitory Effect of Mitomycin C on Proliferation of Primary Cultured Fibroblasts from Human Airway Granulation Tissues

Chen

Zhang

et al. 2013

Respiration

View full text Add to dashboard Cite

Background: Airway granulation tissue and scar formation pose a challenge because of the high incidence of recurrence after treatment. As an emerging treatment modality, topical application of mitomycin C has potential value in delaying the recurrence of airway obstruction. Several animal and clinical studies have already proven its feasibility and efficacy. However, the ideal dosage has still not been determined. Objectives: To establish a novel method for culturing primary fibroblasts isolated from human airway granulation tissue, and to investigate the dose-effect of mitomycin C on the fibroblast proliferation in vitro, so as to provide an experimental reference for clinical practitioners. Methods: Granulation tissues were collected during the routine bronchoscopy at our department. The primary fibroblasts were obtained by culturing the explanted tissues. The cells were treated with different concentrations of mitomycin C (0.1, 0.2, 0.4, 0.8 and 1.6 mg/ml) for 5 min followed by additional 48-hour culture before an MTT assay was performed to measure cell viability. Results: MTT assay showed that mitomycin C reduced cell viability at all tested concentrations. The inhibitory ratios were 10.26, 26.77, 32.88, 64.91 and 80.45% for cells treated with mitomycin C at 0.1, 0.2, 0.4, 0.8 and 1.6 mg/ml, respectively. Conclusions: Explant culture is a reliable method for culturing primary fibroblasts from human airway granulation tissue, and mitomycin C can inhibit proliferation of the fibroblasts in vitro.

show abstract

Acquired subglottic stenosis—depth and not extent of the insult is key

Cited by 58 publications

References 27 publications

A model of canine tracheal stenosis induced by radiofrequency cauterization

A model of canine tracheal stenosis induced by radiofrequency cauterization

Tracheal Stenosis Induction: A Comparison of Open Versus Endoscopic Methods in a Rabbit Model

Inhibitory Effect of Mitomycin C on Proliferation of Primary Cultured Fibroblasts from Human Airway Granulation Tissues

Contact Info

Product

Resources

About