Acoustotaxis – in vitro stimulation in a wound healing assay employing surface acoustic waves

Stamp, Melanie; Brugger, Manuel S.; Wixforth, Achim; Westerhausen, Christoph

doi:10.1039/c6bm00125d

Cited by 36 publications

(45 citation statements)

References 39 publications

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“…Amongst others, we investigated an osseointegration model with Saos-2 cells [ 23 ]. Just recently, we also demonstrated the positive effects of very low amplitude SAW on the stimulation of cell migration [ 24 ]. Our data clearly exhibits the SAW induced, dynamic mechanical and electrical stimulation and directly promotes the cell growth.…”

Section: Introductionmentioning

confidence: 97%

Controllable Acoustic Mixing of Fluids in Microchannels for the Fabrication of Therapeutic Nanoparticles

et al. 2016

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Fifteen years ago, surface acoustic waves (SAW) were found to be able to drive fluids and numerous applications in microfluidics have been developed since. Here, we review the progress made and report on new approaches in setting-up microfluidic, continuous flow acoustic mixing. In a microchannel, chaotic advection is achieved by generation of a SAW driven fluid jet perpendicular to the mean flow direction. Using a high speed video camera and particle image velocimetry, we measure the flow velocities and show that mixing is achieved in a particularly controllable and fast way. The mixing quality is determined as a function of system parameters: SAW power, volume flux and fluid viscosity. Exploring the parameter space of mixing provides a practical guide for acoustic mixing in microchannels and allows for adopting conditions to different solvents, as e.g., required for the generation of nanoscale particles from alcoholic phases. We exemplarily demonstrate the potential of SAW based continuous flow mixing for the production of therapeutic nucleic acid nanoparticles assembled from polymer and lipid solutions.

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Section: Introductionmentioning

confidence: 97%

Controllable Acoustic Mixing of Fluids in Microchannels for the Fabrication of Therapeutic Nanoparticles

et al. 2016

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“…Several groups have already studied cell adhesion under static and dynamic conditions [14,15,16,17,18,19,20]. One of the first approaches to dynamically study cell adhesion used a rotating disk in a fluid above a substrate with adhered cells to generate a shear force field [14].…”

Section: Introductionmentioning

confidence: 99%

“…However, due to its construction, the method does not allow for in situ observation of the cells, which hinders a detailed time-dependent investigation and high amounts of sample volume are needed in the setup’s recent development stage [15]. In contrast, the technique of surface acoustic wave (SAW)-induced streaming has been shown to enable the construction of much smaller setups to study cell adhesion with respect to shear flow [16,17,18]. Bussonnière et al investigated the de-adhesion of cells directly from a piezoelectric substrate [16].…”

Section: Introductionmentioning

confidence: 99%

“…However, presuming the cells to adhere directly on the piezoelectric substrate prohibits the investigation of different substrate materials. Furthermore, the cells are not only exposed to shear flow but also to electric, thermal and mechanic influences of the propagating SAW which has been shown to alter the cells’ behavior [17]. We recently developed a SAW-activated system that works with small sample volumes and allows to decouple the single environmental influences from each other.…”

Section: Introductionmentioning

confidence: 99%

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Exploring the Limits of Cell Adhesion under Shear Stress within Physiological Conditions and beyond on a Chip

et al. 2016

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Cell adhesion processes are of ubiquitous importance for biomedical applications such as optimization of implant materials. Here, not only physiological conditions such as temperature or pH, but also topographical structures play crucial roles, as inflammatory reactions after surgery can diminish osseointegration. In this study, we systematically investigate cell adhesion under static, dynamic and physiologically relevant conditions employing a lab-on-a-chip system. We screen adhesion of the bone osteosarcoma cell line SaOs-2 on a titanium implant material for pH and temperature values in the physiological range and beyond, to explore the limits of cell adhesion, e.g., for feverish and acidic conditions. A detailed study of different surface roughness Rq gives insight into the correlation between the cells’ abilities to adhere and withstand shear flow and the topography of the substrates, finding a local optimum at Rq = 22 nm. We use shear stress induced by acoustic streaming to determine a measure for the ability of cell adhesion under an external force for various conditions. We find an optimum of cell adhesion for T = 37 °C and pH = 7.4 with decreasing cell adhesion outside the physiological range, especially for high T and low pH. We find constant detachment rates in the physiological regime, but this behavior tends to collapse at the limits of 41 °C and pH 4.

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“…The wound healing assay was performed by following the protocol provided in previous literature ( 20 ). RPE cells were cultured in DMEM in a 12-well plate and treated with Si-TGF-β1 or Si-vector for 48 h at 37°C.…”

Section: Methodsmentioning

confidence: 99%

LYTAK1 attenuates proliferation of retinal pigment epithelial cells through TGF‑β‑mediated epithelial‑mesenchymal transition via the ERK/AKT signaling pathway

Chen

Yan

et al. 2017

Exp Ther Med

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Retinal pigment epithelial (RPE) cells have crucial roles in the initiation and development of human ophthalmic diseases. Our previous study suggested that transforming growth factor-β (TGF-β)-activated kinase 1 (TAK1) is a potential target in the progression and pathogenesis of human proliferative vitreoretinopathy disease. The present study further analyzed the role of TAK1 inhibitor, LYTAK1, in human RPE cells and explored the potential molecular mechanism of LYTAK1-mediated proliferation of human RPE cells. Proliferation of human RPE cells was investigated following treatment with LYTAK1 and knockdown of TGF-β. TGF-β-mediated epithelial-mesenchymal transition (EMT) through regulation of the extracellular signal-regulated kinase (ERK)/protein kinase B (AKT) signaling pathway was also explored to analyze the LYTAK1-mediated mechanism of proliferation in human RPE cells. The present results demonstrated that LYTAK1 administration suppressed TAK1 gene and protein expression in human RPE cells. LYTAK1 administration also inhibited proliferation and migration of human RPE cells in vitro. Outcomes indicated that LYTAK1 treatment downregulated expression levels of TGF-β1 and EMT markers, including cadherin, fibronectin and α-smooth muscle actin in human RPE cells. Notably, results demonstrated that the ERK/AKT signal pathway was blocked by LYTAK1 in human RPE cells. Knockdown of TGF-β markedly inhibited phosphorylation and activity of TAK1 and suppressed the LYTAK1-mediated ERK/AKT signaling pathway in RPE cells, which further canceled inhibition of RPE cell proliferation by LYTAK1. In conclusion, these findings indicated that LYTAK1 may inhibit RPE cell proliferation through the TGF-β-mediated EMT/ERK/AKT signaling pathway, suggesting that TAK1 may be a potential target for the treatment of RPE diseases.

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Acoustotaxis – in vitro stimulation in a wound healing assay employing surface acoustic waves

Cited by 36 publications

References 39 publications

Controllable Acoustic Mixing of Fluids in Microchannels for the Fabrication of Therapeutic Nanoparticles

Controllable Acoustic Mixing of Fluids in Microchannels for the Fabrication of Therapeutic Nanoparticles

Exploring the Limits of Cell Adhesion under Shear Stress within Physiological Conditions and beyond on a Chip

LYTAK1 attenuates proliferation of retinal pigment epithelial cells through TGF‑β‑mediated epithelial‑mesenchymal transition via the ERK/AKT signaling pathway

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