Abstract. The Yugur folk songs with distinctive ethnic characteristics. Traditional folk songs retain more features of minority folk songs, and have important research value and cultural value. The eastern folk songs due to historical and geographical factors, and in the process of language integration, with the influence of Mongolian folk songs, the folk songs produced unique singing characteristics. This paper uses the research the method of speech acoustics, research on Eastern Yugur traditional folk songs singing voice timbre, including two aspects of singer's formant and singing voice frequency characteristics. The study concluded that the folk songs singing timbre features and singer singing characteristics.