Aclimatación ex vitro de plántulas de Fragaria x ananassa Duch.

Juárez, María Concepción Valencia; López, Diana Escobedo; Espino, Luis Febronio Díaz; Pérez, Enrique González

doi:10.29312/remexca.v10i1.1633

Cited by 3 publications

(1 citation statement)

References 13 publications

(20 reference statements)

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“…'Winter Star' en concentración de sales del 50% de MS suplementado con IBA 1,5 mgL -1 se obtuvo mayor número y longitud de raíces en comparación con el medio de cultivo sin reguladores de crecimiento; además indujo la formación de raíces en menor número de días (Mehta et al, 2021). Asimismo, en la variedad 'Nikte' con MS suplementado con 30 gL -1 de sacarosa, 7 gL -1 de agar + 0,2 mgL -1 de BAP, produjo 4,3 raíces por vitroplanta y una longitud de 2,8 cm, superior a otros tratamientos utilizados (Valencia et al, 2019); mientras que, en la variedad 'Chandler', en MS al 50% + 1,0 mgL -1 de IBA se obtuvo 5,8 brotes por vitroplanta y 1,0 mgL -1 de tidiazuron + 2,0 mgL -1 de kinetina no indujo a la formación de raíces (Irshad et al, 2023). En la presente investigación, el medio MS fue sin dilución y que posteriores trabajos se podría disminuir la concentración y así se reduciría el monto de inversión en la micropropagación de estas tres variedades.…”

Section: Multiplicación Y Enraizamientounclassified

Role of BA and IAA on regeneration and shoot proliferation in vitro of three strawberry (Fragaria x ananassa Duch.) varieties

Gonzales-Arteaga,

Rodríguez-Layza,

Romero-Rivas

et al. 2023

Agroind. sci.

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The objective was to determine the response of three strawberrry varieties (Fragaria x ananassa Duch.) to the aplication of two plant growth regulators: indol acetic acid (IAA) and 6-Benzyl aminopurine (BAP) added to Murashige & Skoog médium in strawberry micropropagation. The complete randomized block design was used and three strawberry varieties ‘Aromas’, ‘Albion’ and ‘Camarosa’ were considered as trials. MS medium for shoot regeneration in initial stage was supplemented with BAP 0.5 mgL-1 and IAA 0.6 mgL-1. Shoot proliferation MS medium was supplemented with BAP 0.5 mgL-1 in multiplication stage and MS medium hormone free was used for rooting induction. It was determined that after 32 days of cultivation the combination of IAA and BAP significantly affected shoot regeneration of apical shoot tips of three strawberry varieties. The greatest effect was caused on ‘Camarosa’. In addition, results on shoot multiplication using BAP indicated that ‘Albion’ performed better in shoot proliferation in terms of shoots per explants reaching 2.51 compared to ‘Camarosa’ and ‘Aromas’ with significant differences. However rooting medium with no growth regulators improves root development with best response of ‘Camarosa’ root length reaching 4.76 cm.

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Section: Multiplicación Y Enraizamientounclassified

Role of BA and IAA on regeneration and shoot proliferation in vitro of three strawberry (Fragaria x ananassa Duch.) varieties

Gonzales-Arteaga,

Rodríguez-Layza,

Romero-Rivas

et al. 2023

Agroind. sci.

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In vivo adaptation of regenerant plants of Fragaria vesca L. cultivars

Чорнобров

Tkachova

2020

Plant Varieties Studying and Protection

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The adaptation of regenerant plants to environmental conditions is the final stage of micropropagation. According to a number of authors, when in vitro plants are transferred to in vivo non-sterile conditions, a significant percentage of mortality is recorded. In a previous publication, the regenerative capacity of strawberry (Fragaria vesca L.) in vitro tissues on MS culture medium (Murashige & Skoog, 1962) and a regenerants was obtained (Chornobrov O. Yu., 2019). The objective of the study is to develop an optimal protocol of acclimation of in vitro F. vesca plants to in vivo conditions. Methods. Biotechnological and statistical methods of research were applied. For the research 'Ruiana' and 'Zhovte Dyvo' cultivars were used with in vitro cultivation cycle of 30-35 days. Prepared plants were planted in 0.33 L plastic contai ners, one piece in a mixture of coconut substrate and perlite (3:1). Plants were kept under high relative humidity (85-90%) conditions for 3-5 days, 6-8 days and 10-14 days. The studies were carried out in the Plant Biotechnology Laboratory of SS of NULES of Ukraine "BFRS" during 2019-2020. Results. The duration of Fragaria vesca regenerant plants exposure in conditions of high relative humidity significantly affected adaptation efficiency. The proportion of 'Ruiana' and 'Zhovte Dyvo' plants adapted to the greenhouse conditions were 47.6 ± 2.5% and 60.0 ± 1.7%, respectively, when the plants were kept for 10-14 days. A significant efficiency of plant adaptation (more than 70%) was obtained under condition of preliminarily exposure the roots of the plants in an auxin solution for 25-30 minutes with daily application of 30% solution of glycerine as foliar spray. The plants adapted to the greenhouse conditions had pigmentation characteristic of the variety, without signs of chlorosis and vitrification. Conclusions. An optimal protocol for in vitro adaptation of F. vesca cultivars to in vivo conditions was developed and viable plants were obtained. Further research will be aimed at studying the growth and development of F. vesca regenerant plants in open ground.

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An Optimized Protocol for Micropropagation and Acclimatization of Strawberry (Fragaria × ananassa Duch.) Variety ‘Aroma’

et al. 2022

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In strawberry micropropagation, several challenges must be overcome to obtain quality plants and achieve high survival rate to ex vitro acclimatization. In this study, therefore, a set of protocols were evaluated to optimize explant (meristem) disinfection, in vitro growth (multiplication and rooting), and ex vitro acclimatization of strawberry. The results showed that explants treated with 1.0% NaClO for 5 min had a lower percentage of contamination, and achieved a higher percentage of viability, height, and number of leaves. In vitro growth was favored by the use of 1 mg L−1 zeatin, since it allowed greater seedling growth (number of shoots, seedling height, number of leaves, number of roots and root length), and a higher pre-acclimation rate (100%). In the acclimatization phase, plants grown in a substrate composed of compost + peat combined with 4 g of humic acid achieved better response in morphological and physiological variables. In fact, the results of this study could be used to cultivate strawberry plants of the ‘Aroma’ variety with high commercial quality.

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Aclimatación ex vitro de plántulas de Fragaria x ananassa Duch.

Cited by 3 publications

References 13 publications

Role of BA and IAA on regeneration and shoot proliferation in vitro of three strawberry (Fragaria x ananassa Duch.) varieties

Role of BA and IAA on regeneration and shoot proliferation in vitro of three strawberry (Fragaria x ananassa Duch.) varieties

In vivo adaptation of regenerant plants of Fragaria vesca L. cultivars

An Optimized Protocol for Micropropagation and Acclimatization of Strawberry (Fragaria × ananassa Duch.) Variety ‘Aroma’

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