Acinetobacter indicus sp. nov., isolated from a hexachlorocyclohexane dump site

Abstract

The taxonomic position of a Gram-negative, non-motile, oxidase negative and catalase positive strain, A648 T , isolated from a hexachlorocyclohexane (HCH) dump site located in Lucknow, India, was ascertained by using a polyphasic approach. A comparative analysis of a partial sequence of the rpoB gene and the 16S rRNA gene sequence revealed that strain A648T belonged to the , were found to be less than 8 %. The major cellular fatty acids of strain A648T were 18 : 1v9c (19.6 %), summed feature 3 (15.9 %), 16 : 0 (10.6 %) and 12 : 0 (6.4 %). The DNA G+C content was 40.4 mol%. The polar lipid profile of strain A648 T indicated the presence of diphosphatidylglycerol, phosphatidylethanolamine, followed by phosphatidylglycerol and phosphatidylcholine. The predominant polyamine of strain A648 T was 1,3-diaminopropane and moderate amounts of putrescine, spermidine and spermine were also detected. The respiratory quinone consisted of ubiquinone with nine isoprene units (Q-9). On the basis of DNA-DNA hybridization, phenotypic characteristics and chemotaxonomic and phylogenetic comparisons with other members of the genus Acinetobacter, strain A648 T is found to be a novel species of the genus Acinetobacter, for which the name Acinetobacter indicus sp. nov. is proposed. The type strain is A648 T (5DSM 25388 T 5CCM 7832 T ).

Tessaracoccus flavus sp. nov., isolated from the drainage system of a lindane-producing factory

“…We have already characterized several novel bacterial species from stressed environments including fly ash dumping sites of thermal power plants in Delhi and Kanpur, India (Prakash & Lal, 2006;Prakash et al, 2007), oil-contaminated site, Panipat oil refinery, India (Gupta et al, 2008(Gupta et al, , 2009, and a hexachlorocyclohexane dumpsite, situated at Ummari village, Lucknow, India (Kumar et al, 2008(Kumar et al, , 2015Singh & Lal, 2009;Verma et al, 2009Verma et al, , 2015Sharma et al, 2010;Malhotra et al, 2012;Dwivedi et al, 2013;Singh et al, 2013Singh et al, , 2014Singh et al, , 2015Mahato et al, 2015). To further analyse the bacteria from the hexachlorocyclohexane dumpsite, samples were collected from the drainage system of a lindane-producing unit (Lucknow, Uttar Pradesh, India; 268 549 N 818 099 E).…”

Acinetobacter puyangensis sp. nov., isolated from the healthy and diseased part of Populus ×euramericana canker bark

“…Cells grown in TSB for 24 h at 30 u C were prepared and analysed as described by Komagata & Suzuki (1987) by using the Sherlock Identification System (MIDI) (Sasser et al, 2005). Major fatty acids of the two strains were 9-octadecenoic acid (C 18 : 1 v9c, 39.87 %), hexadecanoic acid (C 16 : 0 , 11.26 %) and summed feature 3 (comprising C 16 : 1 v7c/C 16 : 1 v6c, 18.90 %) and were similar to those of species of the genus Acinetobacter (Kampfer, 1993;Kim et al, 2008;Malhotra et al, 2012).…”

Fluviicoccus keumensis gen. nov., sp. nov., isolated from freshwater

“…n. Fluviicoccus river-dwelling coccus). (Franzmann & Skerman, 1981); 4, Paraperlucidibaca (Oh et al, 2011;Yoon et al, 2013); 5, Alkanindiges illinoisensis MVAB Hex1 T (Bogan et al, 2003;Song et al, 2008); 6, Acinetobacter (Bouvet & Grimont, 1986;Nishimura et al, 1988;Nemec et al, 2001Nemec et al, , 2003Carr et al, 2003;Juni & Bøvre, 2005;Song et al, 2008;Malhotra et al, 2012). DPG, diphosphatidylglycerol; PE, phosphatidylethanolamine; PG, phosphatidylglycerol.…”