Acinetobacter
               
            
            puyangensis sp. nov., isolated from the healthy and diseased part of Populus ×euramericana canker bark

Li, Yong; Piao, Chun‐gen; Ma, Yue; He, Wei; Wang, Haiming; Chang, Ju-pu; Guo, Limin; Wang, Xi-zhuo; Xie, Shou-jiang; Guo, Min-wei

doi:10.1099/ijs.0.047274-0

Cited by 25 publications

(11 citation statements)

References 17 publications

(20 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…For 16S rRNA gene sequence analysis, PCR amplification was carried out with primers 8F/1525R (5′-AGAGTTTGATCCTGGCTCAG-3′/5′-AAGGAGGTGATCCAGCC-3′) as described by Lane (1991) and Baker et al (2003). A 1400 bp sequence, corresponding to nucleotide positions 53–1453 in the Escherichia coli numbering system, was used for sequence similarity calculations (Li et al , 2013) using the clustal w program with LaserGene software 7 ( dnastar ). The 16S rRNA gene sequences of the reference strains were retrieved from GenBank.…”

mentioning

confidence: 99%

Brenneria populi sp. nov., isolated from symptomatic bark of Populus×euramericana canker

Fang

Han

et al. 2015

International Journal of Systematic and Evolutionary Microbiology

Self Cite

View full text Add to dashboard Cite

Five Gran-stain-negative, facultatively anaerobic, motile, bacterial strains were isolated from symptomatic bark tissue of Populus¾euramericana canker. Strains grew at 4-41 6C, pH 4-10 and 0-6 % (w/v) salinity. They were positive with respect to catalase activity and negative for oxidase activity, nitrate reduction and the Voges-Proskauer reaction. Analysis of 16S rRNA gene sequences indicated that these five poplar isolates belong to the genus Brenneria, having highest sequence similarity of 95.98 % with Brenneria goodwinii LMG 26270 T . These five isolates formed a single cluster based on multilocus sequence analysis, indicating that they all belong to a single taxon within the genus Brenneria, which was confirmed by DNA-DNA hybridization. The DNA G+C content was 54.9-55.7 mol%, and the main fatty acids were C 16 : 0 , C 18 : 1 v7c, C 17 : 0 cyclo and C 16 : 1 v7c/iso-C 15 : 0 2-OH. Based on these results, we describe a novel species of the genus Brenneria with the proposed name Brenneria populi sp. nov. The type strain is D9-5 T (5CFCC 11963 T 5KCTC 42088 T ).The genus Brenneria was first established by Hauben et al. (1998a). At the time of writing, the genus Brenneria comprises five species with validly published names. Almost all species of the genus are plant pathogens. Brenneria salicis (the type species of the genus) causes willow (Salix spp.) watermark disease, and occurs mainly in the xylem vessels of the host plants (Hauben et al., 1998b). There are also three species which can cause plant bark canker disease. Brenneria nigrifluens and Brenneria rubrifaciens are the causal agents of bark canker and deep bark canker of walnut, respectively (Loreti et al., 2008;McClean et al., 2008). Brenneria alni is the causal agent of bark canker of alder (Surico et al., 1996). Moreover, another two species, namely Brenneria goodwinii and 'Brenneria roseae' (including two subspecies), which were isolated from symptomatic oak tissues, are both associated with acute oak decline (Denman et al., 2012;Brady et al., 2014). Here, we describe the phenotypic and genotypic properties of five novel strains representing the genus Brenneria isolated from symptomatic bark of Populus6euramericana canker in Henan Province of China. The Populus6euramericana canker with abundant white, sour exudates on poplar trees more than five years old were observed for the first time in China's Henan and Shandong provinces in 2006. Diseased plants had stem or branch bark that cracked and exuded frothy fluid. When the disease progressed, many cankers (50-15063-8 cm, width by length) appeared rapidly (Li et al., 2014). Lonsdalea quercina subsp. populi proved to be the causal agent of the bark canker (Li et al., 2014).During our research on pathogen isolation from Populus6euramericana canker, besides the pathogen L. quercina subsp. populi, five other strains (D9-5 T 5CFCC

show abstract

mentioning

confidence: 99%

Brenneria populi sp. nov., isolated from symptomatic bark of Populus×euramericana canker

Fang

Han

et al. 2015

International Journal of Systematic and Evolutionary Microbiology

Self Cite

View full text Add to dashboard Cite

show abstract

“…For example, more than 100 bacterial species (including more than 10 undescribed species) were isolated and identified from 50 samples. We have recently described three species from those samples, Acinetobacter puyangensis, Acinetobacter qingfengensis and Brenneria populi (Li et al 2013(Li et al , 2014b. In this study, we describe a strain designated 2-bin T isolated from one of the 50 symptomatic bark samples of a P. 9 euramericana canker.…”

Section: Introductionmentioning

confidence: 96%

Lampropedia puyangensis sp. nov., isolated from symptomatic bark of Populus × euramericana canker and emended description of Lampropedia hyalina (Ehrenberg 1832) Lee et al. 2004

Wang

Piao

et al. 2015

Antonie van Leeuwenhoek

Self Cite

View full text Add to dashboard Cite

A Gram-stain negative, Neisser-stain negative, aerobic, non-motile, non-spore-forming, slimy, glossy bacterial strain with single or clustered coccoid cells and white colony colour, designated as 2-bin(T), was isolated from cankered bark tissue of Populus × euramericana. The strain was found to grow at 15-40 °C and pH 5-10, with an optimum of 30 °C and pH 8.0. The strain was found to be negative with respect to catalase and positive for oxidase activity, nitrate reduction and Voges-Proskauer reaction. Analysis of 16S rRNA gene sequence data indicated that the isolate belongs to the genus Lampropedia, having sequence similarity of 96.24 % with Lampropedia hyalina ATCC11041(T). DNA-DNA relatedness of strain 2-bin(T) with L. hyalina JCM 21380(T) was 26.7 ± 4.6 %. The DNA G+C content of strain 2-bin(T) was determined to be 57 % and the major cellular fatty acids were identified as C16:0, C16:1 ω7c/C16:1 ω6c and C18:1 ω7c. The polar lipid profile of strain 2-bin(T) was found to contain diphosphatidylglycerol, a glycolipid, phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol, a phospholipid, phosphatidylmonomethylethanolamine and three unidentified lipids (L1, L2, L3). Based on molecular data and physiological and biochemical characteristics, strain 2-bin(T) is considered to represent a novel species in the genus Lampropedia, for which the name Lampropedia puyangensis sp. nov. is proposed. The type strain is 2-bin(T) (= CFCC 10925(T) = KCTC 32235(T)).

show abstract

“…The bacterium A. baumannii is an opportunistic pathogen (Gram-negative bacilli bacteria) which causing world wide nosocomial infections included different of hospital acquired infection (UTI, endocarditis, surgical-site infections, meningitis, septicemia, and ventilator-associated pneumonia among patients in ICU) (1,2,3). These bacteria develop resistance against different antibiotics like carbapenems group that's most effective antimicrobial agents for the treatment of infections caused by multidrug resistant bacteria (MDR) (4,5,6).…”

Section: Introductionmentioning

confidence: 99%

Genotyping of fusA Gene from Clinical Isolates Acinetobacter baumannii in Baghdad

Journal¹

2018

Baghdad Sci.J

View full text Add to dashboard Cite

This study aims at detecting the differences in genotyping of coding region fusA gene in clinical isolates of Acinetobacter baumannii from Baghdad, Iraq. Collected two hundred clinical samples (50 samples from urine, 50 samples from wound, 50 samples from sputum and 50 samples from otitis infections). Laboratory diagnosis for bacterial isolates carried out by some biochemical tests and confirmed by using VITEK- 2 compact system. The results appeared that twenty isolates of Acinetobacter baumannii in all these samples. Genotyping study was performed of coding region fusA gene of the extracted genome of all bacterial isolates and used specific primers in achieved amplification process of this target gene. DNA sequencing of this gene and alignment of sequencing in NCBI was achieved and drew phylogenetic tree by using Geneious 9 software among locally isolates alone and then among locally isolates and high identity global isolates in GenBank. The results in phylogenetic tree of fusA gene in locally isolates showed 4 groups of isolates included more than one source of isolation. The results in phylogenetic tree of the locally and global isolates showed that are four different groups and each group included some locally isolates and global isolates except group A (AE_22, AE_26) and group E (AE_35, AE_32, AE_33) that not identity with global isolates. The nucleotides sequence of fusA gene from localized isolate (AE_35) was registered in national GenBank under accession number (LOCUS KY818057) and protein ID "ARV90995.1.

show abstract

Acinetobacter puyangensis sp. nov., isolated from the healthy and diseased part of Populus ×euramericana canker bark

Cited by 25 publications

References 17 publications

Brenneria populi sp. nov., isolated from symptomatic bark of Populus×euramericana canker

Brenneria populi sp. nov., isolated from symptomatic bark of Populus×euramericana canker

Lampropedia puyangensis sp. nov., isolated from symptomatic bark of Populus × euramericana canker and emended description of Lampropedia hyalina (Ehrenberg 1832) Lee et al. 2004

Genotyping of fusA Gene from Clinical Isolates Acinetobacter baumannii in Baghdad

Contact Info

Product

Resources

About