Acidosis modifies effects of phosphorylated tropomyosin on the actin-myosin interaction in the myocardium

Kopylova, Galina V.; Matyushenko, Alexander M.; Berg, Valentina Y.; Levitsky, Dmitrii I.; Bershitsky, Sergey Y.; Shchepkin, Daniil V.

doi:10.1007/s10974-020-09593-4

Cited by 2 publications

(1 citation statement)

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“…We observed more remarkable T1D-induced changes in the phosphorylation of sarcomeric proteins in LV compared to IS and RV (Table 2). A decrease in RLC phosphorylation might be responsible for reduced CM tension in diabetic LV [15,51], while increased Tpm and cMyBP-C phosphorylation might serve to protect LV from ischemic injury [52,53].…”

Section: Interventricular Alterations Promoted By Type 1 Diabetesmentioning

confidence: 99%

Type 1 Diabetes Impairs Cardiomyocyte Contractility in the Left and Right Ventricular Free Walls but Preserves It in the Interventricular Septum

Khokhlova

Myachina

Volzhaninov

et al. 2022

IJMS

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Type 1 diabetes (T1D) leads to ischemic heart disease and diabetic cardiomyopathy. We tested the hypothesis that T1D differently affects the contractile function of the left and right ventricular free walls (LV, RV) and the interventricular septum (IS) using a rat model of alloxan-induced T1D. Single-myocyte mechanics and cytosolic Ca2+ concentration transients were studied on cardiomyocytes (CM) from LV, RV, and IS in the absence and presence of mechanical load. In addition, we analyzed the phosphorylation level of sarcomeric proteins and the characteristics of the actin-myosin interaction. T1D similarly affected the characteristics of actin-myosin interaction in all studied regions, decreasing the sliding velocity of native thin filaments over myosin in an in vitro motility assay and its Ca2+ sensitivity. A decrease in the thin-filament velocity was associated with increased expression of β-myosin heavy-chain isoform. However, changes in the mechanical function of single ventricular CM induced by T1D were different. T1D depressed the contractility of CM from LV and RV; it decreased the auxotonic tension amplitude and the slope of the active tension–length relationship. Nevertheless, the contractile function of CM from IS was principally preserved.

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