Type 1 Diabetes Impairs Cardiomyocyte Contractility in the Left and Right Ventricular Free Walls but Preserves It in the Interventricular Septum

Khokhlova, Anastasia; Myachina, Tatiana; Volzhaninov, Denis; Butova, Xenia; Kochurova, Anastasia M.; Berg, Valentina Y.; Гетте, И. Ф.; Moroz, Gleb A.; Klinova, Svetlana V.; Minigalieva, Ilzira A.; Solovyova, Olga; Данилова, И. Г.; Sokolova, Ksenia; Kopylova, Galina V.; Shchepkin, Daniil V.

doi:10.3390/ijms23031719

Cited by 6 publications

(4 citation statements)

References 58 publications

(65 reference statements)

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“…Huxley et al [ 4 ] conducted a meta-analysis and reported that men with T1DM have a mortality ratio of 5.7 attributed to CVD, while women have a mortality ratio of 11.3. The mechanisms underlying CVD in T1DM are multifactorial, involving reduced oxygen availability [ 5 ], excessive free fatty acid accumulation [ 6 ], impaired glucose metabolism [ 7 ], autonomic neuropathy [ 8 ], and impaired cardiac function [ 9 ].…”

Section: Introductionmentioning

confidence: 99%

RETRACTED: Non-neuronal cholinergic system delays cardiac remodelling in type 1 diabetes

Munasinghe

Saw

Reily-Bell

et al. 2023

Heliyon

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Section: Introductionmentioning

confidence: 99%

RETRACTED: Non-neuronal cholinergic system delays cardiac remodelling in type 1 diabetes

Munasinghe

Saw

Reily-Bell

et al. 2023

Heliyon

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“…Several pathological processes, including diabetes, lead to a change in the properties of cardiomyocytes [ 25 , 26 ]. A recently published work describes the different effects of DM on the cardiomyocyte morphology of individual ventricles [ 27 ]. This could explain why the difference in the expression of NPBWR1 caused by diabetes was detected just in LV.…”

Section: Discussionmentioning

confidence: 99%

Regulation of Prepro-NeuropeptideW/B and Its Receptor in the Heart of ZDF Rats: An Animal Model of Type II DM

Pandey

Jarkovská

Tůma

et al. 2022

IJMS

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Neuropeptide B (NPB) and neuropeptide W (NPW) are neuropeptides, which constitute NPB/W signaling systems together with G-protein coupled receptors NPBWR1. The location and function of NPB/W signaling systems have been predominantly detected and mapped within the CNS, including their role in the modulation of inflammatory pain, neuroendocrine functions, and autonomic nervous systems. The aim of the study is to investigate the impact of diabetes on the neuropeptide B/W signaling system in different heart compartments and neurons which innervates it. In the RT-qPCR analysis, we observed the upregulation of mRNA for preproNPB in RV, for preproNPW in LA, and for NPBWR1 in DRG in diabetic rats. On the contrary, the expression of mRNA for NPBWR1 was downregulated in LV in diabetic rats. In the WB analysis, significant downregulation of NPBWR1 in LV (0.54-fold, p = 0.046) in diabetic rats was observed at the proteomic level. The presence of NPBWR1 was also confirmed in a dissected LCM section of cardiomyocytes and coronary arteries. The positive inotropic effect of NPW described on the diabetic cardiomyocytes in vitro could point to a possible therapeutic target for compensation of the contractile dysfunction in the diabetic heart. In conclusion, the NPB/W signaling system is involved in the regulation of heart functions and long-term diabetes leads to changes in the expression of individual members of this signaling system differently in each cardiac compartment, which is related to the different morphology and function of these cardiac chambers.

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“…Sarcomere dynamics in mechanically non-loaded atrial and ventricular cardiomyocytes were assessed using a confocal laser scanning microscopy system (LSM 710, Carl Zeiss, Jena, Germany), as described previously [ 58 ]. In brief, myocytes were placed in a chamber on the microscope stage and superfused with a modified Tyrode solution.…”

Section: Methodsmentioning

confidence: 99%

The Acute Effects of Leptin on the Contractility of Isolated Rat Atrial and Ventricular Cardiomyocytes

Khokhlova

Myachina

Butova

et al. 2022

IJMS

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Leptin is a pleiotropic peptide playing an important role in the regulation of cardiac functions. It is not clear whether leptin directly modulates the mechanical function of atrial cardiomyocytes. We compared the acute effects of leptin on the characteristics of mechanically non-loaded sarcomere shortening and cytosolic Ca2+ concentration ([Ca2+]i) transients in single rat atrial and ventricular cardiomyocytes. We also studied the functional properties of myosin obtained from cardiomyocytes using an in vitro motility assay and assessed the sarcomeric protein phosphorylation. Single cardiomyocytes were exposed to 5, 20, and 60 nM leptin for 60 min. In ventricular cardiomyocytes, 60 nM leptin depressed sarcomere shortening amplitude and decreased the rates of shortening and relaxation. These effects were accompanied by a decrease in the phosphorylation of cMyBP-C, an increase in Tpm phosphorylation, and a slowdown of the sliding velocity of thin filaments over myosin in the in vitro motility assay. In contrast, in atrial cardiomyocytes, the phosphorylation of cMyBP-C and TnI increased, and the characteristics of sarcomere shortening did not change. Leptin had no effect on the characteristics of [Ca2+]i transients in ventricular cardiomyocytes, while 5 nM leptin prolonged [Ca2+]i transients in atrial cardiomyocytes. Thus, leptin-induced changes in contractility of ventricular cardiomyocytes may be attributed to the direct effects of leptin on cross-bridge kinetics and sarcomeric protein properties rather than changes in [Ca2+]i. We also suggest that the observed differences between atrial and ventricular cardiomyocytes may be associated with the peculiarities of the expression of leptin receptors, as well as signaling pathways in the atrial and ventricular myocardium.

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Type 1 Diabetes Impairs Cardiomyocyte Contractility in the Left and Right Ventricular Free Walls but Preserves It in the Interventricular Septum

Cited by 6 publications

References 58 publications

RETRACTED: Non-neuronal cholinergic system delays cardiac remodelling in type 1 diabetes

RETRACTED: Non-neuronal cholinergic system delays cardiac remodelling in type 1 diabetes

Regulation of Prepro-NeuropeptideW/B and Its Receptor in the Heart of ZDF Rats: An Animal Model of Type II DM

The Acute Effects of Leptin on the Contractility of Isolated Rat Atrial and Ventricular Cardiomyocytes

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