Type 1 Diabetes Impairs Cardiomyocyte Contractility in the Left and Right Ventricular Free Walls but Preserves It in the Interventricular Septum

Khokhlova, Anastasia; Myachina, Tatiana; Volzhaninov, Denis; Butova, Xenia; Kochurova, Anastasia M.; Berg, Valentina Y.; Гетте, И. Ф.; Moroz, Gleb A.; Klinova, Svetlana V.; Minigalieva, Ilzira A.; Solovyova, Olga; Данилова, И. Г.; Sokolova, Ksenia; Kopylova, Galina V.; Shchepkin, Daniil V.

doi:10.3390/ijms23031719

Cited by 7 publications

(6 citation statements)

References 60 publications

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“…Huxley et al [ 4 ] conducted a meta-analysis and reported that men with T1DM have a mortality ratio of 5.7 attributed to CVD, while women have a mortality ratio of 11.3. The mechanisms underlying CVD in T1DM are multifactorial, involving reduced oxygen availability [ 5 ], excessive free fatty acid accumulation [ 6 ], impaired glucose metabolism [ 7 ], autonomic neuropathy [ 8 ], and impaired cardiac function [ 9 ].…”

Section: Introductionmentioning

confidence: 99%

RETRACTED: Non-neuronal cholinergic system delays cardiac remodelling in type 1 diabetes

Munasinghe

Saw

Reily-Bell

et al. 2023

Heliyon

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Section: Introductionmentioning

confidence: 99%

RETRACTED: Non-neuronal cholinergic system delays cardiac remodelling in type 1 diabetes

Munasinghe

Saw

Reily-Bell

et al. 2023

Heliyon

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“…Several pathological processes, including diabetes, lead to a change in the properties of cardiomyocytes [ 25 , 26 ]. A recently published work describes the different effects of DM on the cardiomyocyte morphology of individual ventricles [ 27 ]. This could explain why the difference in the expression of NPBWR1 caused by diabetes was detected just in LV.…”

Section: Discussionmentioning

confidence: 99%

Regulation of Prepro-NeuropeptideW/B and Its Receptor in the Heart of ZDF Rats: An Animal Model of Type II DM

Pandey

Jarkovská

Tůma

et al. 2022

IJMS

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Neuropeptide B (NPB) and neuropeptide W (NPW) are neuropeptides, which constitute NPB/W signaling systems together with G-protein coupled receptors NPBWR1. The location and function of NPB/W signaling systems have been predominantly detected and mapped within the CNS, including their role in the modulation of inflammatory pain, neuroendocrine functions, and autonomic nervous systems. The aim of the study is to investigate the impact of diabetes on the neuropeptide B/W signaling system in different heart compartments and neurons which innervates it. In the RT-qPCR analysis, we observed the upregulation of mRNA for preproNPB in RV, for preproNPW in LA, and for NPBWR1 in DRG in diabetic rats. On the contrary, the expression of mRNA for NPBWR1 was downregulated in LV in diabetic rats. In the WB analysis, significant downregulation of NPBWR1 in LV (0.54-fold, p = 0.046) in diabetic rats was observed at the proteomic level. The presence of NPBWR1 was also confirmed in a dissected LCM section of cardiomyocytes and coronary arteries. The positive inotropic effect of NPW described on the diabetic cardiomyocytes in vitro could point to a possible therapeutic target for compensation of the contractile dysfunction in the diabetic heart. In conclusion, the NPB/W signaling system is involved in the regulation of heart functions and long-term diabetes leads to changes in the expression of individual members of this signaling system differently in each cardiac compartment, which is related to the different morphology and function of these cardiac chambers.

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“…Measurements of auxotonic force generated by mechanically loaded atrial CM were performed as described elsewhere ( 27 , 28 ). Briefly, two pairs of carbon fibers (≈10 µm in diameter, Tsukuba Materials Information Laboratory, Japan) were attached to the top and bottom surfaces of the left and right CM ends by electrostatic forces.…”

Section: Methodsmentioning

confidence: 99%

The inter-chamber differences in the contractile function between left and right atrial cardiomyocytes in atrial fibrillation in rats

Butova

Myachina

Simonova

et al. 2023

Front. Cardiovasc. Med.

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IntroductionThe left and right atria (LA, RA) work under different mechanical and metabolic environments that may cause an intrinsic inter-chamber diversity in structure and functional properties between atrial cardiomyocytes (CM) in norm and provoke their different responsiveness to pathological conditions. In this study, we assessed a LA vs. RA difference in CM contractility in paroxysmal atrial fibrillation (AF) and underlying mechanisms.MethodsWe investigated the contractile function of single isolated CM from LA and RA using a 7-day acetylcholine (ACh)-CaCl2 AF model in rats. We compared auxotonic force, sarcomere length dynamics, cytosolic calcium ([Ca2+]i) transients, intracellular ROS and NO production in LA and RA CM, and analyzed the phosphorylation levels of contractile proteins and actin-myosin interaction using an in vitro motility assay.ResultsAF resulted in more prominent structural and functional changes in LA myocardium, reducing sarcomere shortening amplitude, and velocity of sarcomere relengthening in mechanically non-loaded LA CM, which was associated with the increased ROS production, decreased NO production, reduced myofibrillar content, and decreased phosphorylation of cardiac myosin binding protein C and troponin I. However, in mechanically loaded CM, AF depressed the auxotonic force amplitude and kinetics in RA CM, while force characteristics were preserved in LA CM.DiscussionThus, inter-atrial differences are increased in paroxysmal AF and affected by the mechanical load that may contribute to the maintenance and progression of AF.

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Type 1 Diabetes Impairs Cardiomyocyte Contractility in the Left and Right Ventricular Free Walls but Preserves It in the Interventricular Septum

Cited by 7 publications

References 60 publications

RETRACTED: Non-neuronal cholinergic system delays cardiac remodelling in type 1 diabetes

RETRACTED: Non-neuronal cholinergic system delays cardiac remodelling in type 1 diabetes

Regulation of Prepro-NeuropeptideW/B and Its Receptor in the Heart of ZDF Rats: An Animal Model of Type II DM

The inter-chamber differences in the contractile function between left and right atrial cardiomyocytes in atrial fibrillation in rats

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