Ácido acetilsalicílico (AAS) inhibe angiogénesis en membrana alantocorionica de pollo (MAC)

Abstract

This study shows that ASA in therapeutic concentrations has an anti-angiogenic effect in a physiologic model in vivo.

FTIR Characterization of Sulfated Polysaccharides Obtained from Macrocystis integrifolia Algae and Verification of Their Antiangiogenic and Immunomodulatory Potency In Vitro and In Vivo

“…The microvascular density was determined by counting all blood vessels per 9000 µm 2 (30 µm × 300 µm) in an optical microscope, with a magnification of 400× and an eyepiece located 600 µm from the filter. Counts were carried out in each of the work groups, obtaining sample values of the number of blood capillaries [ 73 , 74 ].…”

“…At the end of the incubation, the D-MEM/F12 medium was removed from the plates with CSFEP treated with sulfated polysaccharides, and 22 μL of the MTT solution was added to each well and incubated at 37 °C until a change in color was observed. A total of 100 μL of dimethylsulfoxide (DMSO) was added to solubilize the formazan salts in all wells, and incubation was performed for 8 min; then, the absorbance measurement was taken at 570 nm in the ELISA reader [ 73 , 74 ].…”

Efecto Angiogénico Comparado del Celecoxib Microencapsulado en PLGA v/s no Encapsulado En un Ensayo in vivo de Angiogénesis

“…Se utilizaron 60 Huevos fertilizados White Leghorn provenientes del Instituto de Salud Pública, Chile. Se procedió al protocolo descrito por Córdova et al (2016). A las 48 horas de incubación se perforó cada huevo y se extrajo aproximadamente 2 mL de albúmina con ayuda de una pipeta Pasteur.…”

Expresión de VEGF-A y VEGFR2 en Miocardio de Pollos Tratados con Ácido Acetilsalicílico (AAS)

“…Aplicación de AAS y AS. Se utilizaron 30 huevos de pollo de raza White Leghorn embrionados, incubados con aire humedecido en una cámara a 37 ºC, para analizar la capacidad angiogénica, de acuerdo a lo descrito previamente por Córdova et al (2016). Brevemente, a las 48 horas de incubación se perforó cada huevo y se extrajo aproximadamente 2 mL de albúmina con ayuda de una pipeta Pasteur.…”