Acidification modulates the traffic of Trypanosoma cruzi trypomastigotes in Vero cells harbouring Coxiella burnetii vacuoles

Parameters affecting cellular invasion and escape from the parasitophorous vacuole by different infective forms of Trypanosoma cruzi

“…a: T 1/2 formation: half-life for parasitophorous vacuole formation. As indicated in the text, a mean error of ± 1 h is assumed for each determination; b: mean time of residence; c: T 1/2 escape: half-life for parasitophorous escape Whereas hemolysin activity has been shown to be pH sensitive and hence inhabitable by pH elevation (Ley et al 1990) this activity is apparently lacking in metacyclics of the G as well as of CL (Andreoli & Mortara 2003) strains. Unexpectedly, amastigotes of the G strain (this study) as well as of the Y strain (Ley et al 1990) that express pH sensitive hemolysin are not affected by raising the cytoplasmic pH of the target cell (Fig.…”

Mammalian cell invasion and intracellular trafficking by Trypanosoma cruzi infective forms

“…We have also recently obtained evidence that about 20% of CL strain (T. cruzi II) metacyclic trypomastigotes may also recruit the early endosome antigen EEA-1 when invading Vero cells harboring the bacterium Coxiella burnetii (Andreoli and Mortara 2003a).…”

“…So far we have been able to identify TcTOX activities in isolated extracellular amastigotes (Stecconi-Silva et al 2003) and tissue-culture derived trypomastigotes (Andreoli and Mortara 2003a). In contrast, metacyclic trypomastigotes display very weak transialidase activity and undetectable TcTOX Mortara 2003a, Stecconi-Silva et al 2003).…”

Specificity ofLegionella pneumophilaandCoxiella burnetiiVacuoles and Versatility ofLegionella pneumophilaRevealed by Coinfection

“…Mature C. burnetii replication vacuoles readily fuse with vacuoles harboring the intracellular pathogens Mycobacterium avium (15,18), Mycobacterium tuberculosis (18), Leishmania amazonensis (51), and Trypanosoma cruzi (2). Of these organisms, M. avium and L. amazonensis efficiently replicate in this environment (18,51).…”