Acidic vesicles in living hyphae of an arbuscular mycorrhizal fungus, Gigaspora margarita

Cell-based measurements to assess physiological status of Pseudo-nitzschia multiseries, a toxic diatom

“…FSC and BOPIDY uptake were also closely 284 correlated with these previous parameters, indicating that increased DA production was 285 associated with a higher intracellular lipid content. A factorial plan ( The concentration used was 100 times higher than the one used for fungus (Saito et al, 2004) 344 but in agreement with those on human muscle (Wolins et al, 2001) and lower than the one 345 used on amoeba (Kosta et al, 2004). 346…”

Temporal and spatial variations of lipid droplets during adipocyte division and differentiation

“…BODIPY 493/503, a specific probe for intracellular LDs (29), was dissolved in DMSO to produce a 5 mM stock solution similar to the method used in a previous report (30). Hoechst 33342 was dissolved in sterilized distilled-deionized water to produce a 2 mg/ml stock solution.…”

pH measurement of tubular vacuoles of an arbuscular mycorrhizal fungus, Gigaspora margarita

“…The vacuolar profiles resemble those stained with Oregon Green 488 carboxylic acid diacetate 8 (carboxy-DFFDA) (Uetake et al 2002;Saito et al 2004) that have been used as a selective probe for fungal vacuoles (Cole et al 1998;Uetake et al 2002). A pH-mapping image of tubular vacuoles in a G. margarita germ tube is presented in Fig.…”

“…For this study, fluorescent images of AM fungal vacuoles were acquired using a laser-scanning confocal microscope to acquire high-resolution images of fine tubular vacuoles in germ tubes. The germ tubes are useful for cytological studies because of easy handling of fresh materials under a microscope (Uetake et al 2002;Saito et al 2004). Their physiological properties and vacuolar organization are likely to resemble those of extraradical hyphae (Yao et al 2010).…”