Cell-based measurements to assess physiological status of Pseudo-nitzschia multiseries, a toxic diatom

Lelong, Aurélie; Hégaret, Hélène; Soudant, Philippe

doi:10.1016/j.resmic.2011.06.005

Cited by 22 publications

(21 citation statements)

References 68 publications

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“…Cells thus acquired less energy that could be allocated to cell division or stored as lipid. Primary metabolism was enhanced during the first days of growth, as already demonstrated by Lelong et al [19], who showed that the beginning of DA production by P. multiseries is associated with an increase in esterase activity. It may thus be assumed that most of the energy, perhaps coming mainly from lipid reserves because photosynthesis was low, was allocated to DA production in P. multiseries cultures containing an alien bacterial community.…”

Section: Discussionsupporting

confidence: 70%

“…However, only a portion of the SYTOX Green-stained cells were really dead at this stage, as indicated by the high percentage of active cells. Approximately 20% of the cells were dead in untreated cultures of P. multiseries [19], which may be attributable to the age of the isolate. Nonetheless, both species experienced the same AB treatment, thus the cultures with and without added bacteria can be compared.…”

Section: Discussionmentioning

confidence: 99%

“…Measurement of DA production was used as an indicator of the secondary metabolism in P. multiseries . Storage of extra energy in the form of lipid was measured using the BODIPY 493/503 probe [17,18,19]. Thus, allocation of energy to primary metabolism and cell division, secondary metabolism, or energy storage could be monitored during the progression of the diatom culture.…”

Section: Introductionmentioning

confidence: 99%

“…Thus, allocation of energy to primary metabolism and cell division, secondary metabolism, or energy storage could be monitored during the progression of the diatom culture. Cell death was also monitored, using the SYTOX Green probe [17,18,19]. Bacteria were counted and their survival over time was also monitored [19].…”

Section: Introductionmentioning

confidence: 99%

“…Cell death was also monitored, using the SYTOX Green probe [17,18,19]. Bacteria were counted and their survival over time was also monitored [19]. These measurements allowed us to investigate Pseudo-nizschia cell responses to different bacterial communities and to determine how DA production and physiology were modified depending on the added bacterial community.…”

Section: Introductionmentioning

confidence: 99%

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Link between Domoic Acid Production and Cell Physiology after Exchange of Bacterial Communities between Toxic Pseudo-nitzschia multiseries and Non-Toxic Pseudo-nitzschia delicatissima

2014

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Bacteria are known to influence domoic acid (DA) production by Pseudo-nitzschia spp., but the link between DA production and physiology of diatoms requires more investigation. We compared a toxic P. multiseries to a non-toxic P. delicatissima, investigating links between DA production, physiological parameters, and co-occurring bacteria. Bacterial communities in cultures of both species were reduced by antibiotic treatment, and each of the diatoms was inoculated with the bacterial community of the other species. The physiology of P. delicatissima was minimally affected by the absence of bacteria or the presence of alien bacteria, and no DA was detected. P. multiseries grew faster without bacteria, did not produce a significant amount of DA, and exhibited physiological characteristics of healthy cells. When grown with alien bacteria, P. multiseries did not grow and produced more DA; the physiology of these cells was affected, with decreases in chlorophyll content and photosynthetic efficiency, an increase in esterase activity, and almost 50% mortality of the cells. The alien bacterial community had morphological and cellular characteristics very different from the original bacteria, and the number of free-living bacteria per algal cell was much higher, suggesting the involvement of bacteria in DA production.

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Section: Discussionsupporting

confidence: 70%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Link between Domoic Acid Production and Cell Physiology after Exchange of Bacterial Communities between Toxic Pseudo-nitzschia multiseries and Non-Toxic Pseudo-nitzschia delicatissima

2014

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Effect of trace metal‐limited growth on the postmortem dissolution of the marine diatom Pseudo‐nitzschia delicatissima

Boutorh

Moriceau

Gallinari

et al. 2016

Global Biogeochemical Cycles

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We investigated the effects of iron (Fe) and copper (Cu) limitations on biogenic silica (bSiO2) dissolution kinetics of the marine diatom Pseudo‐nitzschia delicatissima during a 3 week batch dissolution experiment. The dissolution of this species was faster during the first week than thereafter. Modeling results from four dissolution models and scanning electron microcopy images suggested the successive dissolution of two phases of bSiO2, with two different dissolution constants. Micronutrient limitation during growth affected the respective proportion of the two phases and their dissolution constants. After 3 weeks of dissolution, frustules from micronutrient‐limited diatoms were better preserved than those of replete cells. Our results also confirm that micronutrient‐limited cells may export more Si relative to N than replete cells and may increase the silicate pump: This may not only be due to a higher degree of silicification of the live cells but also to a decoupling between the recycling of Si and N during dissolution. We suggest that a mechanistic understanding of the evolution of the dissolution constant during dissolution is needed. This would improve the parameterization of dissolution in ecosystem models, and ultimately their predictions on the amount of bSiO2 that dissolves in the photic zone, and the amount of bSiO2 that is exported to the seafloor.

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An Ocean of Signals: Intracellular and Extracellular Signaling in Diatoms

Creveld

Mizrachi

Vardi

2022

The Molecular Life of Diatoms

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Cell-based measurements to assess physiological status of Pseudo-nitzschia multiseries, a toxic diatom

Cited by 22 publications

References 68 publications

Link between Domoic Acid Production and Cell Physiology after Exchange of Bacterial Communities between Toxic Pseudo-nitzschia multiseries and Non-Toxic Pseudo-nitzschia delicatissima

Link between Domoic Acid Production and Cell Physiology after Exchange of Bacterial Communities between Toxic Pseudo-nitzschia multiseries and Non-Toxic Pseudo-nitzschia delicatissima

Effect of trace metal‐limited growth on the postmortem dissolution of the marine diatom Pseudo‐nitzschia delicatissima

An Ocean of Signals: Intracellular and Extracellular Signaling in Diatoms

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