Acidic polysaccharides isolated from Phellinus linteus induce phenotypic and functional maturation of murine dendritic cells

Park, Soonkew; Kim, Gi‐Young; Lim, Jae-Young; Kwak, Jong‐Young; Bae, Yoe‐Sik; Lee, Jae-Dong; Oh, Yang-Hyo; Ahn, Soon‐Cheol; Park, Yeong‐Min

doi:10.1016/j.bbrc.2003.10.136

Cited by 71 publications

(42 citation statements)

References 32 publications

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“…This observation urges critical revision of inconsistency in DC maturation studies, of which some have demonstrated minimal CD80 and/or CD86 upregulation of GM or GM/IL-4 DCs (37-41), whereas others have shown intermediate to maximal activation (27,42,43) after TLR stimulation. In accordance with the maturation data, the capacity of late-stage DCs to stimulate allogeneic T cells is considerably reduced in MLR.…”

Section: Discussionmentioning

confidence: 98%

Reactive Oxygen Species Level Defines Two Functionally Distinctive Stages of Inflammatory Dendritic Cell Development from Mouse Bone Marrow

Sheng

Pietersz²,

Tang³

et al. 2010

The Journal of Immunology

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Reactive oxygen species (ROS) have been implicated in various physiological activities. However, their role in dendritic cell (DC) activation and generation has not been investigated. Using the bone marrow-derived GM-CSF–induced ex vivo DC model, we characterize how induction of ROS correlates with inflammatory DC functionality and expansion. We describe that the functionality of GM-CSF–induced DCs is distinct in two developmental stages. Whereas division of DC-committed hematopoietic progenitor cells (HPCs) neared completion by day 6, the level of ROS soared after day 4. Day 3 ROSlo DCs were highly responsive to TLR stimuli such as LPS and zymosan by rapid upregulation of CD80, CD86, and MHC class II, in contrast to the low response of day 6 ROShi DCs. ROShi DCs could not initiate and sustain a significant level of NF-κB phosphorylation in response to LPS and zymosan, although demonstrating hyperactivation of p38 MAPK by LPS, in a fashion disparate to ROSlo DCs. ROSlo DCs stimulated a higher level of allogeneic and OVA-specific T cell proliferative responses, although ROShi DCs were much more proficient in processing OVA. In response to pathogenic stimuli, ROShi DCs also demonstrated rapid cellular adhesion and H2O2 release, indicating their role in immediate microbial targeting. Moreover, HPC expansion and DC generation were dependent on the surge of ROS in an NADPH oxidase-independent manner. These findings point to the potential role of cellular ROS in mediating functionality and development of DCs from HPCs during inflammation.

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Section: Discussionmentioning

confidence: 98%

Reactive Oxygen Species Level Defines Two Functionally Distinctive Stages of Inflammatory Dendritic Cell Development from Mouse Bone Marrow

Sheng

Pietersz²,

Tang³

et al. 2010

The Journal of Immunology

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“…However, these stimulators are toxic and have limited applications. It has been reported that a number of polysaccharides in plants, including Ganoderma lucidum polysaccharides, Phellinus linteus polysaccharides and Coriolus versicolor polysaccharides, may stimulate the induction of DC maturation (27)(28)(29).…”

Section: Discussionmentioning

confidence: 99%

Lycium barbarum polysaccharides induce Toll-like receptor 2- and 4-mediated phenotypic and functional maturation of murine dendritic cells via activation of NF-κB

Zhu

Zhang

Shen

et al. 2013

Molecular Medicine Reports

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Abstract. Dendritic cells (DCs) are potent antigen-presenting cells that play pivotal roles in the initiation of primary immune responses. Lycium barbarum polysaccharides (LBPs) are known to have a variety of immunomodulatory functions. However, the cellular and molecular mechanisms underlying their therapeutic effects are poorly understood. In this study, we report that LBPs induce phenotypic and functional maturation of DCs. LBPs upregulated DC expression of I-A/I-E and CD11c, enhanced DC allostimulatory activity and induced IL-12p40 production. Furthermore, the activity of LBPs on DCs was significantly reduced by treating the cells with anti-TLR2 or anti-TLR4 antibody prior to LBPs, indicating that both are possible receptors of LBPs. Maturation of DCs by LBPs was able to directly activate the nuclear transcription factor NF-κB p65. The results revealed that LBP stimulation induces the phenotypic and functional maturation of DCs via TLR2-and/or TLR4-mediated NF-κB signaling pathways.

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“…[2][3][4] Although the chemical composition and biological properties of crude polysaccharides from Phellinus spp. have been investigated, [5][6][7] the chemical structure of purified polysaccharides has been determined in only a few cases. Of these, the repeating unit of a 3-O-methylgalactose-containing neutral polysaccharide isolated from cultivated fruit bodies of P. igniarius has been shown to be composed of fucose, galactose, glucose, mannose, and 3-O-Megalactose.…”

mentioning

confidence: 99%

Structural Analysis of a Bioactive Polysaccharide, PISP1, from the Medicinal MushroomPhellinus igniarius

Yang

Zhang

et al. 2009

Bioscience, Biotechnology, and Biochemistry

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Acidic polysaccharides isolated from Phellinus linteus induce phenotypic and functional maturation of murine dendritic cells

Cited by 71 publications

References 32 publications

Reactive Oxygen Species Level Defines Two Functionally Distinctive Stages of Inflammatory Dendritic Cell Development from Mouse Bone Marrow

Reactive Oxygen Species Level Defines Two Functionally Distinctive Stages of Inflammatory Dendritic Cell Development from Mouse Bone Marrow

Lycium barbarum polysaccharides induce Toll-like receptor 2- and 4-mediated phenotypic and functional maturation of murine dendritic cells via activation of NF-κB

Structural Analysis of a Bioactive Polysaccharide, PISP1, from the Medicinal MushroomPhellinus igniarius

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