Acid Nucleases in PSTV-Infected Tomato (Lycopersicum esculentum L.) II. Characterization of Sugar Non-Specific Nuclease Extracted from Healthy and PSTV-Infected Tomato Leaves

Several tomato cultivars were infected with a severe strain of PSTV and a pathophysiological reaction was characterized by means of enzyme-linked immunosorbent assay with serum containing IgGs to disease-associated host-specific leaf proteins. A strong expression of disease-associated symptoms (stunting, epinasty, leaf blade malformation and rugosity) and strong immunochemical reaction was found for cultivars Bizon, Linia, Revermun and Rutgers. The immunochemical assay revealed appearence of a major antigenic protein having a molecular mass of about 70 kD in these cultivars. The immunochemical reaction with disease-associated proteins reached a maximum four weeks after inoculation of PSTV. A weak immunochemical reaction was observed, if proteins from cvs. Sonato, Harzfeuer and Karlik were analyzed. Cvs. Sonato and Harzfeuer did not show characteristic symptoms such as stunting, leaf blade malformation and rugosity. Except for stunting, the same was true for cv. Karlik. On the other hand, no significant difference in PSTV accumulation was observed among the cultivars analyzed.Reciprocal hybrids obtained by crossing cvs. Revermun and Karlik showed strong symptoms of the disease (Revermun-type) and increased activity of nuclease due to PSTV infection. On the contrary, an immunochemical analysis revealed a low level of the disease-associated antigens in these hybrid tomatoes, suggesting rather recessive genetic background determining their expression during PSTV-caused pathogenesis.

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Inhibition of potato spindle tuber viroid (PSTVd) infection with DNA oligonucleotides

Matoušek

Trněná

Arnold³

et al. 1993

Biochimie

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Acid Nucleases in PSTV-Infected Tomato (Lycopersicum esculentum L.) II. Characterization of Sugar Non-Specific Nuclease Extracted from Healthy and PSTV-Infected Tomato Leaves

Cited by 6 publications

References 27 publications

An immunochemical study of antigen expression in potato spindle tuber viroid (PSTV) - infected tomato leaves and calluses

An immunochemical study of antigen expression in potato spindle tuber viroid (PSTV) - infected tomato leaves and calluses

An immunochemical testing of pathophysiological reactions of several PSTVinfected tomato (Lycopersicon esculentum L.) cultivars

Inhibition of potato spindle tuber viroid (PSTVd) infection with DNA oligonucleotides

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