An immunochemical study of antigen expression in potato spindle tuber viroid (PSTV) - infected tomato leaves and calluses

Matoušek, Jaroslav; Dědič, P.; Beneš, Milan J.; Kopáček, Petr; Turková, Věra; Trněná, Ludmila

doi:10.1007/bf02890894

Cited by 2 publications

(5 citation statements)

References 23 publications

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“…These results suggest a rather recessive genetic background, determining its expression and its relative independence on morphological symptoms developed during viroid caused pathogenesis. These observations are in good accordance with previous finding (Matou~ek et al 1990) that the antigen appearence did not correlate with development of a viroid-like syndrome after treating the plants with AgNO3. Granell et al (I 987) used treatments with silver ions for induction of viroid-like symptoms and showed similarity of this phenomenom to viroid caused pathogenesis.…”

Section: Discussionsupporting

confidence: 93%

“…At least one major antigen with a molecular mass of about 70 kD, as estimated under denaturing conditions, was found to be responsible for the immunochemical differences detected among the "sensitive" and "tolerant" cultivars. It has been shown that the investigated protein has accumulated essentially in viroid-infected tomato leaves, but not in tomato leaves infected with potato viruses X, Y, and M (Matou~ek et al 1990). Probably, the detected antigen corresponds to the 70K-subunit of disease-associated tomato leaf protein described also by Galindo et al (1984).…”

Section: Discussionmentioning

confidence: 67%

“…0.1, 1 and 5 Ixg of protein of each variant were absorbed on polystyrene microtiter plates overnight at 4 ~ in 0.2 M sodium bicarbonate buffer, pH 9.6 containing 5 mM 2-mercaptoethanol and 0.2 mM PMSF. Under such conditions of protein coating a preferential reaction with antigens from PSTV-infected leaves is reached in ELISA-test (Matou~ek et al 1990). Incubation of the immobilized antigens with specific IgGs was performed for 2 h at 37 ~ Detection was achieved using goat anti-rabbit IgGs labelled with alkaline phosphatase, p-Nitrophenyl phosphate served as a substrate.…”

Section: Enzyme-linked Lmmunosorbent Assaymentioning

confidence: 99%

“…Viroids are known to be pathogenic RNAs, which do not code for proteins (S~inger 1982). On the other hand, it has been shown, for example, that the potato spindle tuber viroid (PSTV) and the citrus exocortis viroid (CEV) caused in tomatoes the pathogenesis resulting in an accumulation of disease-associated host protein(s) of high antigenicity (Camacho-Henriquez and S/inger 1982, Galindo et al 1984, Granell et al 1987, 1990.…”

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confidence: 99%

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An immunochemical testing of pathophysiological reactions of several PSTVinfected tomato (Lycopersicon esculentum L.) cultivars

Matoušek¹,

Schubert²,

Beneš³

et al. 1991

Biol. plant.

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Several tomato cultivars were infected with a severe strain of PSTV and a pathophysiological reaction was characterized by means of enzyme-linked immunosorbent assay with serum containing IgGs to disease-associated host-specific leaf proteins. A strong expression of disease-associated symptoms (stunting, epinasty, leaf blade malformation and rugosity) and strong immunochemical reaction was found for cultivars Bizon, Linia, Revermun and Rutgers. The immunochemical assay revealed appearence of a major antigenic protein having a molecular mass of about 70 kD in these cultivars. The immunochemical reaction with disease-associated proteins reached a maximum four weeks after inoculation of PSTV. A weak immunochemical reaction was observed, if proteins from cvs. Sonato, Harzfeuer and Karlik were analyzed. Cvs. Sonato and Harzfeuer did not show characteristic symptoms such as stunting, leaf blade malformation and rugosity. Except for stunting, the same was true for cv. Karlik. On the other hand, no significant difference in PSTV accumulation was observed among the cultivars analyzed.Reciprocal hybrids obtained by crossing cvs. Revermun and Karlik showed strong symptoms of the disease (Revermun-type) and increased activity of nuclease due to PSTV infection. On the contrary, an immunochemical analysis revealed a low level of the disease-associated antigens in these hybrid tomatoes, suggesting rather recessive genetic background determining their expression during PSTV-caused pathogenesis.

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Section: Discussionsupporting

confidence: 93%

Section: Discussionmentioning

confidence: 67%

Section: Enzyme-linked Lmmunosorbent Assaymentioning

confidence: 99%

mentioning

confidence: 99%

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An immunochemical testing of pathophysiological reactions of several PSTVinfected tomato (Lycopersicon esculentum L.) cultivars

Matoušek¹,

Schubert²,

Beneš³

et al. 1991

Biol. plant.

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“…In this study, complex expression changes in both common and unique tomato genes were found. Developmental changes in viroidspecific expression patterns were also observed in our early studies using immunochemical techniques (Matouš ek et al, 1990). As a specific case of developmental disorders, down-regulated expression of the expansin LeExp2 gene was found in tomato infected with viroid mutants having a U257A substitution, leading to characteristic flat-top symptoms by restricted cell expansion (Qi and Ding, 2003).…”

Section: Introductionmentioning

confidence: 53%

Accumulation of viroid-specific small RNAs and increase in nucleolytic activities linked to viroid-caused pathogenesis

Matoušek

Kozlová

Orctová

et al. 2007

Biological Chemistry

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Strong viroid-caused pathogenesis was achieved in tomato cv. Rutgers by biolistic transfer of severe or lethal potato spindle tuber viroid (PSTVd) strains, while other tomato genotypes (e.g., Moneymaker) were tolerant. With reciprocal hybrids between sensitive and tolerant genotypes, we show that plant depression dominates over tolerance. Biolistic transfer of the most pathogenic PSTVd strain AS1 to Nicotiana benthamiana, which is considered to be a symptomless PSTVd host, led to a strong pathogenesis reaction and stunting, suggesting the presence of specific viroid pathogenesis-promoting target(s) in this plant species. Total levels of small siRNA-like PSTVd-specific RNAs were enhanced in strongly symptomatic tomato and N. benthamiana plants after biolistic infection with AS1 in comparison to the mild QFA strain. This indicates association of elevated levels of viroid-specific small RNA with production of strong symptoms. In symptom-bearing tomato leaves in comparison to controls, an RNase of approximately 18 kDa was induced and the activity of a nuclease of 34 kDa was elevated by a factor of seven in the vascular system. Sequence analysis of the nuclease cDNA designated TBN1 showed high homology with plant apoptotic endonucleases. The vascular-specific pathogenesis action is supported by light microscopic observations demonstrating a certain lack of xylem tissue and an arrest of the establishment of new vascular bundles in collapsed plants.

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An immunochemical study of antigen expression in potato spindle tuber viroid (PSTV) - infected tomato leaves and calluses

Cited by 2 publications

References 23 publications

An immunochemical testing of pathophysiological reactions of several PSTVinfected tomato (Lycopersicon esculentum L.) cultivars

An immunochemical testing of pathophysiological reactions of several PSTVinfected tomato (Lycopersicon esculentum L.) cultivars

Accumulation of viroid-specific small RNAs and increase in nucleolytic activities linked to viroid-caused pathogenesis

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