Acid‐insoluble human dentin as carrier material for recombinant human BMP‐2

Abstract

The aim of this study is to estimate the increase of bone-inductive potency by human demineralized dentin matrix (DDM) with recombinant human bone morphogenetic protein-2 (BMP-2). Human teeth were crushed, completely demineralized in 0.6M HCl, and freeze-dried. The tooth-derived material is called DDM. The shape of DDM was a particle type and its size varied from 0.4 to 0.8 mm. The BMP-2 dose-dependent study in the rat subcutaneous tissues demonstrated that the volume of induced bone and marrow increased at a dose-dependent manner. The time-course study of bone induction by the BMP-2 (5.0 μg)/DDM (70 mg) was estimated histologically and biochemically. Histological findings showed that the BMP-2/DDM increased bone and marrow sequentially between the DDM particles. Calcium content in the BMP-2/DDM-induced tissue was compatible to the histological findings. ALP activity in the BMP-2/DDM showed a maximal value at 1 week and gradually decreased. The morphometric analysis demonstrated that the BMP-2/DDM showed 66.9%, 79.0% in the volume of bone and marrow, and 32.4%, 21.0% in that of DDM at 8, 32 weeks, respectively. We confirmed that BMP-2 significantly accelerated bone formation in the acid-insoluble human-dentin carriers. These results indicate that human DDM should be an effective carrier for delivering BMP-2 and superior scaffold for bone-forming cells.

Bone induction of human tooth and bone crushed by newly developed automatic mill

“…2) We confirmed histologically that human completely demineralized dentin granules by hand-made method using hammer induced bone and cartilage independently at 4 weeks, 4), 5) and have obtained successful results in clinical studies of the autograft of DDM for bone regeneration.…”

Volumetric, Radiographic, and Histologic Analyses of Demineralized Dentin Matrix Combined with Recombinant Human Bone Morphogenetic Protein-2 for Ridge Preservation: A Prospective Randomized Controlled Trial in Comparison with Xenograft

“…A recent animal study by Kim et al demonstrated that DDM dissolves rhBMP-2 more gradually and steadily than other tricalcium phosphates (TCP) and bovine bone, establishing that particulated DDM is the most effective BMP carrier [31]. Other studies have reported that adding rhBMP-2 to DDM ensures the formation of new bone and cartilage, and DDM is absorbed and replaced by vital new bone tissue approaching almost 80% of the total volume, as indicated by histomorphometric assessment [23,45]. The current study was undertaken to evaluate, using a histomorphometric assessment, whether the addition of rhBMP-2 to DDM leads to superior bone regeneration in comparison with DDM alone or conventional DBBC.…”

Applications of Moldable Autogenous Tooth Bone Graft (M-AutoBT) Mixed with Hydroxypropylmethyl Cellulose for Sinus Lifting

“…DDM can be defined as acid-insoluble, cross-linked collagen with natural growth factors, and is composed of predominantly type I collagen (95 %) and the remaining non-collagenous proteins including small amount of growth factors 1,12) . DDM has potential to promote transformation of mesechymal stem cells into osteoblastic cells, because several well-known cytokines including BMP could be released from dentin through increased surface, enlarged dentinal tubules and loosened collagen fibers by demineralization process 3,13,14) .…”