Acid‐insoluble ash as a marker compound for use in digestibility studies with humans

Rowan, Angela; Moughan, Paul J.; Wilson, Margaret N.

doi:10.1002/jsfa.2740540213

Cited by 15 publications

(8 citation statements)

References 8 publications

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“…The digestibility studies dealing with the comparison of Cr 2 O 3 and AIA as digestibility markers were carried out in chickens (Scott and Boldaji, 1998), pigs (Laerke et al, 2012;Pozza et al, 2013) and also in humans (Rowan et al, 1991). These studies involved monitoring of total tract digestibility, ileal digestibility or both, including studies estimating an ileal endogenous flow of AA and CP (Fan and Sauer, 2002).…”

Section: Discussionmentioning

confidence: 99%

Chromic oxide and acid‐insoluble ash as markers in digestibility studies with growing pigs and sows

Brestenský

Nitrayová

Heger

et al. 2016

Animal Physiology Nutrition

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The results of three experiments, focused on the determination of endogenous ileal flow (EIF) of amino acids (AA) and nitrogen (N) (Exp. 1), apparent ileal digestibility (AID) of AA and N (Exp. 2), and apparent total tract digestibility (ATTD) of dry matter (DM), organic matter (OM), N, calcium (Ca) and phosphorus (P) (Exps. 2 and 3), were used to compare chromic oxide (Cr O ) and acid-insoluble ash (AIA) as digestibility markers. In Exps. 1 and 2, a total of six gilts fitted with T-cannula in terminal ileum, and in Exp. 3, a total of 24 pregnant sows were used. In Exps. 1 and 2, the pigs were assigned into four dietary treatments according to 4 × 6 crossover design (Exp. 1; diets with 0%, 4%, 8% and 12% of casein; Exp. 2 basal diet with different levels of phytase). In Exp. 3, the sows were assigned to four dietary treatments (basal diet with different levels of phytase) of six sows. In Exps. 1 and 2 ileal digesta and in Exps. 2 and 3 faeces were collected for the determination of EIF, AID and ATTD. Differences in EIF of AA determined by Cr O and AIA ranged (p ˃ 0.05) from -4.62 to 4.54%. The lowest EIF was for methionine and the greatest one for proline, determined by both markers. Apparent ileal digestibility determined by Cr O was slightly greater (p ˃ 0.05) in comparison with AIA. Differences ranged from 1.88% (Arg) to 7.08% (Gly). The greatest AID was for arginine and the lowest one for glycine, determined by both Cr O and AIA. Similarly for ATTD of DM, OM, N, Ca and P, there were no differences in digestibility determined by Cr O and AIA. Both, Cr O and AIA, are suitable and comparable markers for digestibility studies in pigs.

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Section: Discussionmentioning

confidence: 99%

Chromic oxide and acid‐insoluble ash as markers in digestibility studies with growing pigs and sows

Brestenský

Nitrayová

Heger

et al. 2016

Animal Physiology Nutrition

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“…The biscuits contained Celite (acid-insoluble ash) as an indigestible marker. The latter substance has been shown to be a suitable marker for use with ileostomates (Rowan et al 1991) and negligible amounts of acid-insoluble ash occur in the typical Western diet. The subjects ate the biscuits over 30 min, and during and following this meal drank sweetened (sucrose) tea or coffee (without milk), water or lemonade ad libitum.…”

Section: Methodsmentioning

confidence: 99%

Endogenous amino acid flow at the terminal ileum of adult humans determined following the ingestion of a single protein‐free meal

1993

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Abstract:The study sought to determine endogenous total nitrogen and amino acid flows at the terminal ileum of adult humans. Six adults (65f 10 kg bodyweight) with well-established ileostomies fasted for 14 h before consuming a single protein-free meal (240 g) containing Celite (acid-insoluble ash) as an indigestible marker. Ileal digesta were collected for 8 h after the meal and endogenous flows were determined by reference to the marker. The mean ( f SE) total 8 h output of digesta dry matter was 9.1 (k3.51) g. The mean ileal flow of total nitrogen was 4.38 (f 0.770), with the mean amino acid flows ranging from 0.17 (fO.009) for methionine to 2.26 (fO.258) for glutamic acid (mg g-' food dry matter intake).

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“…Since it has been recommended that non-PEG-based bowel preparations are used in WGLF studies, however, appropriate normalization methods should be established for these. Some non-absorbable markers that have been employed in fecal studies that could potentially be added to the bowel preparation as a marker in studies include various rare earth elements [29], dysprosium chloride [30], and acid insoluble ash [31].…”

Section: Methods Of Normalizationmentioning

confidence: 99%

Rectal Effluent as a Research Tool

2014

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Studies of localized secretions are generally superior to those of blood because they contain higher concentrations of molecules specific to the organ of interest. A common method used to analyze localized secretions is lavage. The flow of fluid over the lining of a cavity picks up both cells and soluble factors, and the effluent can be collected for study. Gastrointestinal (GI) lavage is easily and noninvasively performed by the administration of gut lavage solutions such as those routinely given to patients prior to colonoscopy, with GI lavage fluid being the copious, watery rectal effluent subsequently induced. Residual effluent is currently suctioned from the colon and discarded during colonoscopy. With millions of routine colonoscopies performed per year, GI lavage fluid is a rich and largely untapped resource for basic and clinical research. Rectal effluent can also be easily collected in a toilet receptacle without need for a colonoscopy. Rectal effluent generated in this manner has been used to study diarrheal disease, mucosal immunology, inflammatory bowel disease, celiac disease, and cancer. It is often referred to as gut lavage, colon lavage, GI lavage, or whole gut lavage fluid, which makes it challenging to locate previous studies in the literature and there are currently no comprehensive reviews of its use as a research tool. This review attempts to fill this void by discussing previous applications of rectal effluent in research and the methods that have been developed for its collection, stabilization, and analysis.

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Acid‐insoluble ash as a marker compound for use in digestibility studies with humans

Cited by 15 publications

References 8 publications

Chromic oxide and acid‐insoluble ash as markers in digestibility studies with growing pigs and sows

Chromic oxide and acid‐insoluble ash as markers in digestibility studies with growing pigs and sows

Endogenous amino acid flow at the terminal ileum of adult humans determined following the ingestion of a single protein‐free meal

Rectal Effluent as a Research Tool

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