Acid and alkaline phosphatase activity in broiler chicks fed with different levels of phytase and non-phytate phosphorus

Abstract

Tartrate-resistant acid (ACP) and alkaline phosphatase (ALP) activities were evaluated in the serum and bone of broiler chicks fed with various amounts of non-phytate phosphorus (NPP) or phytase. Data were analysed using a 4)3 factorial design containing four NPP levels per period. Analyses were performed in chicks aged 1Á21 days (0.21; 0.29; 0.37; 0.45 ppm) and 36Á42 days (0.13; 0.21; 0.29; 0.37 ppm) and under three different phytase level treatments (0, 500 and 1000 FTU/kg) for each period. In 42-day-old animals, the serum ACP and ALP activities did not differ in response to NPP and phytase levels and bone ACP activity decreased with increased phosphorus levels. We observed effects on ALP activity by approximately 70% in lower phosphorus (0.13 and 0.21) levels without phytase. The phytase addition decreased (PB0.05) ALP values in lower phosphorus levels. The bone ALP and ACP levels of 21-day-old animals were not affected by phosphorus or phytase. Pi depletion induces a significant increase in alkaline phosphatase synthesis, suggesting that the function of this enzyme is downregulated by phosphorus.

Reduction of calcium levels in rations supplemented with vitamin D3 or 25-OH-D3 for broilers

“…Roberson and Edwards Jr (1994) reported that TAP activity was not influenced by the addition of phytase when used in low Ca levels and high P levels in broiler feeds. These results are supported by the work of Nakagi et al (2013), who concluded that the highest synthesis and expression of this enzyme is observed in P-deficient diets. Additionally, as reported in review, Schmidt et al (2007) suggested that the elevation of enzymes related to bone metabolism is more sensitive in young animals.…”

The efficacy of 2 phytases on inositol phosphate degradation in different segments of the gastrointestinal tract, calcium and phosphorus digestibility, and bone quality of broilers

“…Similarly, a decrease in dietary Ca from 9.1 to 4.5 g/kg and in total P from 7.2 to 3.7 g/kg linearly decreased dual-energy x-ray absorptiometry-measured total BMD and BMC (Onyango et al, 2003). At day 42, a reduction of dietary avP from 0.37 to 0.13% linearly increased broiler bone tartrate-resistant acid phosphatase concentration (de Souza Nakagi et al, 2013), an indicator of bone resorption activity by osteoclasts (Dacke et al, 1993). Cortical and trabecular bone tissues are actively remodeled throughout the life cycle of broiler chickens (Pratt and Cooper, 2018).…”

Effects of Supplementation of Zinc, Manganese, or Copper and Different Phytase Levels in Serum and Bone Acid and Alkaline Phosphatases of Broiler Chicks

“…The present study was performed using 1,200 male Cobb broilers, raised according to lineage management manual of Cobb broilers (Nakagi et al, 2013). The diets were available ad libitum, containing energy levels of 2,950 and 3,150 kcal ME kg -1 .…”

“…Therefore, exogenous enzymes have been used in non-ruminant diets (Bedford, 2000;Ahmed & Tahir, 2015) as an alternative to reduce the effects of anti-nutritional factors, including phytate, which may act as a chelating agent, present in plant seeds and grains, being major constituents of poultry diets (Greiner & Konietzny, 1999;Oh et al, 2004;Santos et al, 2015). Phosphate released from phytate can improve digestion of this compound and reduce or avoid the use of phosphorus in poultry feed (Khalid et al, 2013;Nakagi et al, 2013). In addition, its hydrolysis is dependent on the sources of phytate, eRBCA-2022-1722 eRBCA-2022-1722 and the impact of phytase on the availability of trace minerals remains to be clarified (Sebastian et al, 1996;Chung et al, 2013;Santos et al, 2015;Manobhavan et al, 2016).…”