Acid and alkaline phosphatase activities in the clam Ruditapes philippinarum

Blasco, Julián; Puppo, J.; Sarasquete, Carmen

doi:10.1007/bf00349392

Cited by 57 publications

(28 citation statements)

References 16 publications

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“…The activity values obtained for AcP were higher than those for ALP in both cases. This agrees with that reported in the literature (Blasco et al 1993), except in regard to C 11 -LAS at the end of the depuration phase (day 7; fig. 5a, b), when an opposite effect was observed in the organisms exposed to the two higher concentrations.…”

Section: Resultados Y Discusiónsupporting

confidence: 93%

“…Los valores de actividad obtenidos para AcP son mayores que para ALP con ambos compuestos, lo que concuerda con la bibliografía (Blasco et al 1993), excepto en el caso de C 11 LAS al final de la depuración (día 7; fig. 5a, b), cuando se observa un efecto contrario en los organismos expuestos a las dos concentraciones mayores.…”

Section: Resultados Y Discusiónunclassified

“…Las actividades fosfatasa ácida y alcalina fueron determinadas por el método descrito por Blasco et al (1993) y los and high levels were 2.4 ± 0.3, 6.7 ± 0.5 and 12.8 ± 0.3 µg g -1 , respectively, while those of NPEO 2.8 were 13.4 ± 0.1, 14.8 ± 0.4 and 31.7 ± 6.6 µg g -1 , respectively. The analytical procedure developed by Sáez et al (2000) was used for the anionic surfactant and that proposed by Zhao et al (1999) for the nonionic.…”

Section: Enzimasmentioning

confidence: 99%

“…The activity of AcP and ALP was determined following the method described by Blasco et al (1993) and the results are expressed in U mg -1 proteins (U = 1 µmol p-nitrophenol min -1 ). The measurements were taken with a Versamax multiplate reader at 405 nm and the values were corrected for the wavelength passage in 350-µL microplates.…”

Section: Enzymesmentioning

confidence: 99%

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Enzymatic activity of acid and alkaline phosphatase and catalase in Ruditapes philippinarum as biomarkers of stress caused by anionic (C11-LAS) and non-ionic (NPEO2.8) surfactants

Álvarez-Muñoz¹,

Sáez²,

Blasco³

et al. 2006

CiencMar

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Actividades enzimáticas de las fosfatasas ácida y alcalina y la catalasa en Ruditapes philippinarum como biomarcadores del estrés generado por tensioactivos aniónicos (C 11 -LAS) y no iónicos (NPEO 2.8 )Enzymatic activity of acid and alkaline phosphatase and catalase in Ruditapes philippinarum as biomarkers of stress caused by anionic (C 11 -LAS) and non-ionic (NPEO 2.8 ResumenSe realizó un estudio de toxicidad subcrónica mediante la exposición de organismos a dos tensioactivos en un sistema en flujo continuo, con un periodo de exposición al tóxico de 120 h, y otro de depuración en ausencia del mismo de 48 h. Los experimentos se realizaron con Ruditapes philippinarum, molusco con amplia distribución en zonas intermareales y de gran importancia económica, utilizando sulfonato de alquilbenceno lineal (LAS) como principal tensioactivo aniónico y el nonilfenol etoxilado (NPEO 2.8 ) como no iónico. Los organismos fueron expuestos a tres concentraciones ambientales (µg L -1 ) de LAS y de NPEO 2.8 , siendo 2.4, 6.7 y 12.8 µg g -1 de LAS las concentraciones medidas en el interior de organismos expuestos a la concentración menor, intermedia y mayor, mientras que las concentraciones de NPEO 2.8 fueron de 13.4 y 14.8 y 31.7 µg g -1 , respectivamente. Las respuestas empleadas como "criterio de efecto" de toxicidad subcelular fueron: las medidas de las actividades enzimáticas de la catalasa (CAT), y las fosfatasas ácida (AcP) y alcalina (ALP). En el caso de CAT, no se han observado diferencias significativas al final de la fase de exposición con ninguno de ambos tensioactivos. Sin embargo, al final de la fase de depuración existió un aumento de la actividad CAT con el C 11 LAS en los organismos expuestos a la menor concentración (P < 0.05) y una disminución significativa con el NPEO 2.8 en los organismos expuestos a la mayor concentración, la misma que corresponde con la mayor acumulación del compuesto en el interior del organismo. En los organismos expuestos a las concentraciones media y mayor de C 11 -LAS se observó inhibición de la actividad AcP y activación de la ALP al final del ensayo (día 7) (P < 0.05). En los organismos expuestos a la mayor concentración de NPEO 2.8 existe un aumento de la actividad específica al final de la fase de depuración.Palabras clave: tensioactivo, fosfatasa ácida, fosfatasa alcalina, catalasa, Ruditapes philippinarum. AbstractA subchronic toxicity study was performed by exposing organisms to two surfactants in a continuous flow-through system for 120 h, followed by a depuration phase of 48 h. The bivalve mollusc Ruditapes philippinarum, widely distributed in intertidal zones and of high commercial value, was used. The main anionic and non-ionic surfactants employed were linear alkylbenzene sulphonate (LAS) and nonylphenol ethoxylate (NPEO 2.8 ), respectively. The organisms were exposed to three environmental levels (µg L -1 ), and the concentrations of C 11 -LAS measured in the tissues were 2.4, 6.7 and 12.8 µg g -1 for the low, intermediate and high exposure levels, while those of NPEO 2...

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Section: Resultados Y Discusiónsupporting

confidence: 93%

Section: Resultados Y Discusiónunclassified

Section: Enzimasmentioning

confidence: 99%

Section: Enzymesmentioning

confidence: 99%

See 2 more Smart Citations

Enzymatic activity of acid and alkaline phosphatase and catalase in Ruditapes philippinarum as biomarkers of stress caused by anionic (C11-LAS) and non-ionic (NPEO2.8) surfactants

Álvarez-Muñoz¹,

Sáez²,

Blasco³

et al. 2006

CiencMar

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“…Although the enzyme from Escherichia coli and human has been extensively studied, little is known about the enzyme from gastropod. A study involving crustaceans showed that ALPase might be involved in calcium secretion [8], while another study on clam suggested that the ALPase activity could be positively related to shell deposition [9]. Another study on response of innate immune factors in Haliotis diversicolor supertexta used ALPase as an indicator, and the results showed that ALPase activity changed when the abalone challenged with Vibrio parahaemolyticus [10].…”

Section: Introductionmentioning

confidence: 99%

Unfolding and Inactivation of Abalone (Haliotis diversicolor) Alkaline Phosphatase During Denaturation by Guanidine Hydrochloride

Liao

Chen

Zhang

et al. 2008

Appl Biochem Biotechnol

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Abalone, a kind of low poikilothermic invertebrate, is easily exposed to ocean environment stress. Since it is one of the important mariculture animals, the attention paid to the abalone study becomes increasing. Alkaline phosphatase (ALPase, EC 3.1.3.1) is a kind of zinc-contained metalloenzyme, which catalyzes the nonspecific hydrolysis of phosphate monoesters. Unfolding and inactivation of ALPase from abalone (Haliotis diversicolor) during denaturation by guanidine hydrochloride (GuHCl) of different concentrations has first been studied. The kinetic theory of the substrate reaction by enzyme was described by Tsou, which was applied to the study on ALPase's kinetic course of inactivation by GuHCl. The result showed that the inactivation of the enzyme by GuHCl was a slow, reversible reaction with fractional remaining activity. The microscopic rate constants were determined. The result, k(+0) > k'(+0), showed that the enzyme was protected by the substrate to a certain extent during guanidine denaturation. The changes of conformation of the enzyme in different concentrations of GuHCl have been studied by means of measuring the fluorescence spectra. The results showed that the inactivation occurred before the noticeable conformational changes of the enzyme molecule as a whole can be detected, which suggests that the active site of the enzyme has more flexibility than the whole enzyme molecule. These studies will facilitate the understanding of physiological and biochemical features of the H. diversicolor and will also help in the understanding of the abalone immune system.

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A fluorescence‐based hydrolytic enzyme activity assay for quantifying toxic effects of Roundup® to Daphnia magna

Ørsted

Roslev

2015

Enviro Toxic and Chemistry

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Daphnia magna is a widely used model organism for aquatic toxicity testing. In the present study, the authors investigated the hydrolytic enzyme activity of D. magna after exposure to toxicant stress. In vivo enzyme activity was quantified using 15 fluorogenic enzyme probes based on 4-methylumbelliferyl or 7-amino-4-methylcoumarin. Probing D. magna enzyme activity was evaluated using short-term exposure (24-48 h) to the reference chemical K2 Cr2 O7 or the herbicide formulation Roundup®. Toxicant-induced changes in hydrolytic enzyme activity were compared with changes in mobility (International Organization for Standardization standard 6341). The results showed that hydrolytic enzyme activity was quantifiable as a combination of whole body fluorescence of D. magna and the fluorescence of the surrounding water. Exposure of D. magna to lethal and sublethal concentrations of Roundup resulted in loss of whole body enzyme activity and release of cell constituents, including enzymes and DNA. Roundup caused comparable inhibition of mobility and alkaline phosphatase activity with median effective concentration values at 20 °C of 8.7 mg active ingredient (a.i.)/L to 11.7 mg a.i./L. Inhibition of alkaline phosphatase activity by Roundup was lowest at 14 °C and greater at 20 °C and 26 °C. The results suggest that the fluorescence-based hydrolytic enzyme activity assay (FLEA assay) can be used as an index of D. magna stress. Combining enzyme activity with fluorescence measurements may be applied as a simple and quantitative supplement for toxicity testing with D. magna.

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Acid and alkaline phosphatase activities in the clam Ruditapes philippinarum

Cited by 57 publications

References 16 publications

Enzymatic activity of acid and alkaline phosphatase and catalase in Ruditapes philippinarum as biomarkers of stress caused by anionic (C11-LAS) and non-ionic (NPEO2.8) surfactants

Enzymatic activity of acid and alkaline phosphatase and catalase in Ruditapes philippinarum as biomarkers of stress caused by anionic (C11-LAS) and non-ionic (NPEO2.8) surfactants

Unfolding and Inactivation of Abalone (Haliotis diversicolor) Alkaline Phosphatase During Denaturation by Guanidine Hydrochloride

A fluorescence‐based hydrolytic enzyme activity assay for quantifying toxic effects of Roundup® to Daphnia magna

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