Achromobacter denitrificans strain SP1 efficiently remediates di(2-ethylhexyl)phthalate

Pradeep, S.; Josh, M. K. Sarath; Devi, R. Sudha; Balachandran, S.; Anderson, Robin C.; Benjamin, Sailas

doi:10.1016/j.ecoenv.2014.10.035

Cited by 60 publications

(20 citation statements)

References 36 publications

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“…Different from Predee, we have detected PA in the degration progress [44]: PA used to be regarded as the key to complete the mineralization of DEHP, as it is easier to be utilized by microorganisms than DEHP [45]. What's more, our reasult is different from Xu et al's reports on Acinetobacter SN13: They didn't have detected DEP in the degradation process, but only did detected MEHP [44,46]. On the one hand, this could be because the reaction was too fast for detecting these intermediates, on the other hand, it may due to a better and unkown degration pathway exised.…”

Section: Discussioncontrasting

confidence: 60%

“…And according to previous papers, it had been proved to be a common and effective pathway in PAEs bioremediation; the grampositive bacteria oxidize and dehydrogenate at the C-3-4 position of phthalic acid to form 3, 4dihydroxy phthalic acid, protocatechins and finally enter TCA cycle [42,43]. Different from Predee, we have detected PA in the degration progress [44]: PA used to be regarded as the key to complete the mineralization of DEHP, as it is easier to be utilized by microorganisms than DEHP [45]. What's more, our reasult is different from Xu et al's reports on Acinetobacter SN13: They didn't have detected DEP in the degradation process, but only did detected MEHP [44,46].…”

Section: Discussionmentioning

confidence: 77%

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Biochemical Pathways and Enhanced Degradation of Dioctyl Phthalate (DEHP) by Sodium Alginate Immobilization in MBR System

Zhang

Luo

et al. 2020

Preprint

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A bacterial strain that could effectively degrade DEHP was isolated from the activated sludge and identified as Bacillus sp. by DNA sequencing. The biochemical degradation pathway of DEHP was further analyzed by GC-MS, and the results showed that DEHP was first decomposed into phthalates (DBP). Diuretic sylycol (DEP) was then generated, and phthalates (PA) were generated by a continuous de-ehelateization reaction. Phthalic acid (PA) was oxidized, dehydrogenated, and decarboxylated into protocatechins. Protocatechins enter the TCA cycle through orthotopic ring opening.　To enhance DEHP degradation, sodium alginate and calcium chloride were used as embedding and cross-linking materials, and the strain was immobilized. The immobilization conditions were optimized via an orthogonal experiment, and the results showed that the optimal immobilization conditions were SA mass fraction of 4%, CaCl2 mass fraction of 5%, ratio of bacteria to SA of 1:1, and the crosslinking time of 6 hours. The immobilized bacteria agent was further applied to MBR systems. The results showed that the removal rate of DEHP (5mg/L) in the system by immobilized bacteria was 91.9%, which is significantly higher than that of free bacteria. The 3, 4-dioxygenase gene and microbial community dynamics were analyzed by q-PCR and Illumina Miseq sequencing. The q-PCR results showed that the number of copies of 3, 4-dioxygenase gene in the immobilized system was significantly higher than that of free bacteria. Illumina Miseq sequencing results showed that Micromonospora, Rhodococcus, Bacteroides and Pseudomonas were the dominant generas in the MBR system. The analysis of bacterial community structure indicated that immobilization technology had a positive impact on the system stability. The results implied that this immobilized technique had potential applications in DEHP wastewater treatment.

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Section: Discussioncontrasting

confidence: 60%

Section: Discussionmentioning

confidence: 77%

Biochemical Pathways and Enhanced Degradation of Dioctyl Phthalate (DEHP) by Sodium Alginate Immobilization in MBR System

Zhang

Luo

et al. 2020

Preprint

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show abstract

“…for a particular period of time under controlled laboratory conditions. The organisms were probably isolated from soil [96][97][98]; sediments from river [75], wetland [99], mangrove [100], or deep ocean [101]; activated sludge [102][103][104], compost [70] or municipal waste contaminated soils [105] or river water [106] or sewage sludge [107] (Table 3).…”

Section: Ex Situ Degradation Of Phthalatementioning

confidence: 99%

A monograph on the remediation of hazardous phthalates

Benjamin

Pradeep

Josh

et al. 2015

Journal of Hazardous Materials

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195

103

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“…2.7 | DEHP degradation studies 2.7.1 | Percentage utilization of DEHP DEHP utilization percentage was quantified by the spectrophotometric method as described by Pradeep et al [24]. A. denitrificans SP1 was cultured in BSM supplemented with DEHP (20 µl) at 28°C.…”

Section: Identification Of Hsls By Liquid Chromatography-quadrupolementioning

confidence: 99%

Quorum sensing mediated response of Achromobacter denitrificans SP1 towards prodigiosin production under phthalate stress

et al. 2020

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Quorum sensing is a density‐dependent chemical process between bacteria, which may be intergenus or intragenus. N‐acyl homoserine lactones (HSLs) are a type of small signaling molecules associated with Gram‐negative bacteria for monitoring their own population density. The present study unveils the mechanism of HSLs in Achromobacter denitrificans SP1 while transforming di(2‐ethylhexyl) phthalate (DEHP) into prodigiosin in a simple basal salt medium. The primary detection of HSLs was done by the colorimetric method. Fourier‐transform infrared spectroscopy and liquid chromatography–mass spectrometry‐quadrupole time‐of‐flight confirmed and identified the HSLs. The maximum production of HSLs was observed between 24 and 72 h of incubation, which is noted to be a peak time of DEHP degradation. A total of 57.2% of DEHP was degraded within 30 h and complete degradation was observed within 72 h of incubation. Regulation in the synthesis of various acyl‐HSL molecules, viz. 3OC6‐HSL in the initial stage of DEHP stress, 3OC8‐HSL, and C10‐HSL during the time of degradation and 3OC12‐HSL on completion of degradation was noticed. The role of HSLs on the production of prodigiosin was confirmed using vanillin as an HSL inhibitor. Through the selective activation of HSL molecules, A. denitrificans SP1 sustain the changing stressful conditions. Supplementation of acyl‐HSL signal molecules may boost up the efficacy of A. denitrificans SP1 in both DEHP degradation and prodigiosin production which offers great potential towards the management of DEHP containing plastic wastes.

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Achromobacter denitrificans strain SP1 efficiently remediates di(2-ethylhexyl)phthalate

Cited by 60 publications

References 36 publications

Biochemical Pathways and Enhanced Degradation of Dioctyl Phthalate (DEHP) by Sodium Alginate Immobilization in MBR System

Biochemical Pathways and Enhanced Degradation of Dioctyl Phthalate (DEHP) by Sodium Alginate Immobilization in MBR System

A monograph on the remediation of hazardous phthalates

Quorum sensing mediated response of Achromobacter denitrificans SP1 towards prodigiosin production under phthalate stress

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