Acetylcholinesterase inhibitory and antioxidant properties of Cyclotrichium niveum, Thymus praecox subsp. caucasicus var. caucasicus, Echinacea purpurea and E. pallida

Antioxidant Activity ofIpomoea batatasL. Lam. Leaf Grown in Continental Croatia and Its Effect on Glutathione Level in Glucose-Induced Oxidative Stress

“…This finding is in accordance with some previous studies that demonstrated superior DPPH radical scavenging activity of ethanolic extract of certain plants over antioxidant activities of extracts obtained by more polar solvents. [10,22] EC 50 value of ethanolic extract in the present study (54.1 μg/ml) was similar to EC 50 reported by Prasanth et al [23] (36.5 μg/ml) for I. batatas grown in India. In a study of Thu et al [11] that investigated RSA of I. batatas leaf from Vietnam, EC 50 was achieved with a sample that contained 4.0 nmol of phenol equivalents.…”

Determination of total phenol content, antioxidant activity and acetylcholinesterase inhibition in selected mushrooms from Turkey

“…Electric eel AChE (Type-VI-S, EC 3.1.1.7, Sigma, USA) was employed as the enzyme source, while acetylthiocholine iodide (Sigma, St. Louis, MO, USA) as substrate and 5,5 0 -dithio-bis(2-nitrobenzoic)acid (DTNB) (Sigma, USA) were also used in the anti-AChE activity determination. All reagents and conditions were same as described recently (Orhan et al, 2009). Experiments were performed in four parallel sets and the results were expressed as average values with S.E.M.…”

UPLC–TOF-MS analysis of Galium spurium towards its neuroprotective and anticonvulsant activities

“…5,5 -Dithio-bis(2-nitrobenzoic) acid (DTNB) was used for the measurement of the anticholinesterase activity. All reagents and conditions were same as described in our previous publications (Orhan et al, 2009). Briefly, in this method, 140 L of sodium phosphate buffer (pH 8.0), 20 L of DTNB, 20 L of the sample solution and 20 L of AChE/BChE solution were added by multichannel automatic pipette (Gilson pipetman, France) in a 96-well microplate and incubated for 15 min at 25 • C. The reaction was then initiated with the addition of 10 L of acetylthiocholine iodide/butyrylthiocholine chloride.…”