Acetylcholinesterase electrochemical biosensors with graphene-transition metal carbides nanocomposites modified for detection of organophosphate pesticides

Abstract

An acetylcholinesterase biosensor modified with graphene and transition metal carbides was prepared to detect organophosphorus pesticides. Cyclic voltammetry, differential pulse voltammetry, and electrochemical impedance spectroscopy were used to characterize the electrochemical catalysis of the biosensor: acetylcholinesterase/chitosan-transition metal carbides/graphene/glassy carbon electrode. With the joint modification of graphene and transition metal carbides, the biosensor has a good performance in detecting dichlorvos with a linear relationship from 11.31 μM to 22.6 nM and the limit of detection was 14.45 nM. Under the premise of parameter optimization, the biosensor showed a good catalytic performance for acetylcholine. Compared to the biosensors without modification, it expressed a better catalytic performance due to the excellent electrical properties, biocompatibility and high specific surface area of graphene, transition metal carbides. Finally, the biosensor exhibits good stability, which can be stored at room temperature for one month without significant performance degradation, and has practical potential for sample testing.

A 3D printable adapter for solid-state fluorescence measurements: the case of an immobilized enzymatic bioreceptor for organophosphate pesticides detection

“…Recently, fast, easy, and cheap methodologies have been successfully developed using different fluorescent biosensors to detect pesticides in environmental matrices [16][17][18][19][20]. The majority of biosensors are based on the inhibition of acetylcholinesterase activity [21][22][23][24][25]. However, these systems present low stability after changes in environmental conditions and low specificity toward inhibitor compounds, possibly reacting with other compounds than OPs.…”

Hybrid Nanobioengineered Nanomaterial-Based Electrochemical Biosensors

“…The resultant platform showed a better catalytic performance due to the excellent electrical properties, biocompatibility, and high surface area of graphene and transition metal carbides nanobioconjugate. In addition, the electrochemical biosensor exhibited a linear response from 11.31 µM to 22.6 nM with LOD of 14.45 nM [165]. Kasturi et al published a novel, high-resolution electrochemical biosensor using AuNP-dotted reduced graphene oxide (rGO/Au) for the detection of the miRNA-122 by DPV.…”

A machine learning colorimetric biosensor based on acetylcholinesterase and silver nanoparticles for the detection of dichlorvos pesticides

“…Sensors for OP pesticides have been developed based on fluorescence immunoassay (FIA) 20 and enzyme-linked immunosorbent assay (ELISA) 21 with good sensitivity and selectivity, however, these methods have some limitations, for example requiring antigen/antibody interactions with strong affinity and high specificity. 22 Recently, electrochemical biosensors [23][24][25][26][27][28] and dual signal sensors using fluorometric and colorimetric read-outs have been developed, based on noble metal nanomaterials like gold and silver nanoparticles. [29][30][31][32] Limitations, in this case, include interfering electroactive materials and additional electrode modification required to get reliable outcomes.…”