Acetylcholinesterase biosensor design based on carbon nanotube-encapsulated polypyrrole and polyaniline copolymer for amperometric detection of organophosphates

Du, Dan; Ye, Xiaoxue; Cai, Jie; Liu, Juan; Zhang, Aidong

doi:10.1016/j.bios.2010.04.018

Cited by 164 publications

(39 citation statements)

References 38 publications

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“…When the immersing time was longer than 12 min, the inhibition curve trended to a stable value. However, the inhibition of carbaryl could not reach 100%, which indicated that the binding sites between pesticides and enzymes could reach an equilibration AChE-CdS-G-CHIT/GCE 2-2000 ng/ml 0.7 ng/ml [51] AChE/PB-CHIT/GCE 2-80 ng/ml, 200-1000 ng/ml 0.6 ng/ml [52] AChE/carbon paste electrodes 1-15 μg/ml 0.4 μg/ml [53] AChE-CHIT/Au 0.005-0.1 μg/ml, 0.5-5 μg/ml 0.003 μg/ml [54] GC/MWCNT/CoPc 66-1322 ng/ml 1.1 ng/ml [55] GC/PANI/MWCNT/AChE 1.98-9.92 μg/ml 0.28 μg/ml [56] AChE/PDDA-MWCNTs-GR/GCE 0.8-50 ng/ml, 50-3000 ng/ml 0.13 ng/ml This work state [50]. Thus, the time of 12 min was selected as the optimum inhibition time.…”

Section: Influence Of Incubation Time On Inhibitionmentioning

confidence: 99%

Acetylcholinesterase Biosensor Based on Poly (diallyldimethylammonium chloride)-multi-walled Carbon Nanotubes-graphene Hybrid Film

et al. 2013

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Abstract:In this paper, an amperometric acetylcholinesterase (AChE) biosensor for quantitative determination of carbaryl was developed. Firstly, the poly (diallyldimethy-lammonium chloride) -multi-walled carbon nanotubes-graphene hybrid film was modified onto the glassy carbon electrode (GCE) surface, then AChE was immobilized onto the modified GCE to fabricate the AChE biosensor. The morphologies and electrochemistry properties of the prepared AChE biosensor were investigated by using scanning electron microscopy, cyclic voltammetry and electrochemical impedance spectroscopy. All variables involved in the preparation process and analytical performance of the biosensor were optimized. Based on the inhibition of pesticides on the AChE activity, using carbaryl as model compounds, the biosensor exhibited low detection limit, good reproducibility and high stability in a wide range. Moreover, the biosensor can also be used for direct analysis of practical samples, which would provide a new promising tool for pesticide residues analysis.

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Section: Influence Of Incubation Time On Inhibitionmentioning

confidence: 99%

Acetylcholinesterase Biosensor Based on Poly (diallyldimethylammonium chloride)-multi-walled Carbon Nanotubes-graphene Hybrid Film

et al. 2013

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“…In the second method, the first monomer is electrochemically polymerized on the electrode surface and used as an electrode for the polymerization of second monomer. Choi and Park [8] showed that these copolymer composites will not only display better properties than that of homopolymers but also overcome the limitation of the rareness of new conjugated-bond-containing monomers. Star et al, Chen et al, Arduini et al, Li et al, proved that the electrical ability as well as mechanical properties of heteropolymers are enhanced compared with pure conducting polymer or nanomaterials [8].…”

Section: Hydrolysis Of Acetylcholinementioning

confidence: 99%

Pesticides Detection Using Acetylcholinesterase Nanobiosensor

2016

Biosens J

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Pesticides are chemical substances used for crop improvement and these substances when degraded leave behind certain toxic residues which can be detected using nano biosensors which are relatively inexpensive, does not require sample pre-treatment and provides sensitive screening. The application of nanobiosensors for detection of organophosphates using acetylcholinesterase is introduced in detail. Future prospects toward the development of selective, sensitive biosensing systems are discussed.

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“…Sotiropoulou and Chaniotakis immobilized AChE by adsorption into nanostructured conductive carbon, which acts as the working electrode 129 . Du et al adsorbed AChE onto the multi-walled carbon nanotubes 130 . Vorčáková et al immobilized AChE via adsorption onto the surface of screen-printed three-electrode sensors for determination of cholinesterase inhibition in the presence of some substituted phenylcarbamates 131 .…”

Section: Adsorptionmentioning

confidence: 99%

Cholinesterase-based biosensors

Štěpánková

Vorčáková

2016

Journal of Enzyme Inhibition and Medicinal Chemistry

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Recently, cholinesterase-based biosensors are widely used for assaying anticholinergic compounds. Primarily biosensors based on enzyme inhibition are useful analytical tools for fast screening of inhibitors, such as organophosphates and carbamates. The present review is aimed at compilation of the most important facts about cholinesterase based biosensors, types of physico-chemical transduction, immobilization strategies and practical applications.

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Acetylcholinesterase biosensor design based on carbon nanotube-encapsulated polypyrrole and polyaniline copolymer for amperometric detection of organophosphates

Cited by 164 publications

References 38 publications

Acetylcholinesterase Biosensor Based on Poly (diallyldimethylammonium chloride)-multi-walled Carbon Nanotubes-graphene Hybrid Film

Acetylcholinesterase Biosensor Based on Poly (diallyldimethylammonium chloride)-multi-walled Carbon Nanotubes-graphene Hybrid Film

Pesticides Detection Using Acetylcholinesterase Nanobiosensor

Cholinesterase-based biosensors

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