Acetylcholinesterase biosensor based on 3-carboxyphenylboronic acid/reduced graphene oxide–gold nanocomposites modified electrode for amperometric detection of organophosphorus and carbamate pesticides

Liu, Tao; Su, Haichao; Qu, Xiangjin; Ju, Peng; Cui, Lin; Ai, Shiyun

doi:10.1016/j.snb.2011.09.059

Cited by 177 publications

(76 citation statements)

References 49 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…10-100 0.1 ng/mL [39] BSA/anti-chlorpyrifos/AuNPs/PANI/MWCNTs/GCE 0.1-40 40-500 0.06 ng/mL This work 6.1659lgC (10 −6 mg/mL) and %ΔI=-22.436+27.235lgC (10 −6 mg/mL), with the correlation coefficients of 0.9913 and 0.9904, respectively. The detection limit was estimated to be 0.06×10 −6 mg/mL at a signal/noise of 3 (S/N=3) between the detection signal of low concentration samples and the noise of blank samples.…”

Section: Amperometric Response Of the Immunosensor To Chlorpyrifos Comentioning

confidence: 80%

A Novel Immunosensor Based on Au Nanoparticles and Polyaniline/Multiwall Carbon Nanotubes/Chitosan Nanocomposite Film Functionalized Interface

2013

View full text Add to dashboard Cite

Abstract:A novel multilayer film based on Au nanoparticles (AuNPs) and polyaniline/carboxylated multiwall carbon nanotubes-chitosan nanocomposite (PANI/MWCNTs/CS) was exploited to fabricate a highly sensitive immunosensor for detecting chlorpyrifos. PANI-coated MWCNTs were prepared by in situ chemical polymerization and carboxylated MWCNTs played an important role in obtaining the thin and uniform coating of PANI resulting in the improved immunosensor response. AuNPs were used as a linker to immobilize chlorpyrifos antibody. The performance of the immunosensor was characterized by means of cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS) and scanning electron microscopy (SEM), respectively. All variables involved in the preparation process and analytical performance of the immunosensor were optimized. Under optimal conditions (antibody concentration: 5 μg/mL, working buffer pH: 6.5, incubation time: 40 min, incubation temperature: 25℃), the immunosensor exhibited a wide linear range from 0.1 to 40 × 10 −6 mg/mL and from 40 × 10 −6 mg/mL to 500 × 10 −6 mg/mL, and with a detection limit of 0.06 × 10 −6 mg/mL, which provided a valuable tool for the chlorpyrifos detection in real samples.

show abstract

Section: Amperometric Response Of the Immunosensor To Chlorpyrifos Comentioning

confidence: 80%

A Novel Immunosensor Based on Au Nanoparticles and Polyaniline/Multiwall Carbon Nanotubes/Chitosan Nanocomposite Film Functionalized Interface

2013

View full text Add to dashboard Cite

show abstract

“…Parameters affecting the biosensor response such as pH, applied potential, enzyme loading and inhibition time were optimized. Moreover, this biosensor had good repeatability and high stability [10].…”

Section: Hydrolysis Of Acetylcholinementioning

confidence: 94%

“…These organic toxins enter animal and human bodies directly or indirectly through the food chain or drinking water, and threat human health by inhibiting acetylcholinesterase which is essential for the function of the central nervous system in humans. So it is of great significance to develop a fast, reliable, economical and analytical method for determination of trace amounts of these pesticides [1].…”

Section: Introductionmentioning

confidence: 99%

Pesticides Detection Using Acetylcholinesterase Nanobiosensor

2016

Biosens J

View full text Add to dashboard Cite

Pesticides are chemical substances used for crop improvement and these substances when degraded leave behind certain toxic residues which can be detected using nano biosensors which are relatively inexpensive, does not require sample pre-treatment and provides sensitive screening. The application of nanobiosensors for detection of organophosphates using acetylcholinesterase is introduced in detail. Future prospects toward the development of selective, sensitive biosensing systems are discussed.

show abstract

“…In addition to the already mentioned limitations encountered in chromatography techniques, the other problem related to the activity of the acetylcholinesterase, which is irreversibly inhibited by organo-phosphorous compounds. Electrochemical analytical techniques, based on chemically modified sensors have proved to be a sensitive and selective process for the determination of organo-phosphorous compounds (Cao et al, 2015;Kumaravel and Chandrasekaran, 2015;Liu et al, 2011;Mulchandani et al, 2001a,b;Mulchandani et al, 1999).…”

Section: Introductionmentioning

confidence: 99%

Nano Fe<sub>3</sub>O<sub>4</sub>–graphite paste modified electrochemical sensor for phosphatic pesticide -chlorpyrifos

Hunde¹,

Berhe²,

Tadese³

et al. 2017

mejs

View full text Add to dashboard Cite

A sensitive, cost effective and selective electrochemical sensor has been developed by exploiting iron oxide nano-particles as modifier in the paste of graphite powder. Cyclic voltammetric (CV) and differential pulse voltammetric (DPV) working parameters have been standardized and used practically for the determination of chlorpyrifos (O, O-diethyl O-3, 5, 6-trichloropyridin-2-yl phosphorothioate) pesticide from its aqueous solutions. Both techniques were deployed to investigate the electro-chemical interactions between chlorpyrifos and modified carbon paste sensor along with the redox characteristics at analyte/ sensor interface. The significant enhancement in peak current signals and the improved magnitude of the redox peak potential indicated the awe-inspiring facilitation of the electron transfer process by the modifier at the sensor/ analyte interface. The difference in the redox -potential (ΔEp) and peak current ratio (Ipc/Ipa) have revealed a notable surface enhancement characteristic of the modifier that responds the higher concentration gradient of pesticide at the interface. In lower analyte's concentration range i.e. from 1.0 to 100μM, the peak current varies directly to the pesticide concentration with detection limit of 2.8 x10 -6 mol/L. The relative stability of the modified sensor is fine and the reproducibility of the results is up to 98%, even after a gap of two months. The proposed analytical method is quite successful when applied for the quantification of chlorpyrifos from its aqueous samples. The electrochemical sensing/ detection of the pesticide chlorpyrifos is confirmed by its two electrons redox behavior and the same have been explained by suitable reaction scheme as:

show abstract

Acetylcholinesterase biosensor based on 3-carboxyphenylboronic acid/reduced graphene oxide–gold nanocomposites modified electrode for amperometric detection of organophosphorus and carbamate pesticides

Cited by 177 publications

References 49 publications

A Novel Immunosensor Based on Au Nanoparticles and Polyaniline/Multiwall Carbon Nanotubes/Chitosan Nanocomposite Film Functionalized Interface

A Novel Immunosensor Based on Au Nanoparticles and Polyaniline/Multiwall Carbon Nanotubes/Chitosan Nanocomposite Film Functionalized Interface

Pesticides Detection Using Acetylcholinesterase Nanobiosensor

Nano Fe<sub>3</sub>O<sub>4</sub>–graphite paste modified electrochemical sensor for phosphatic pesticide -chlorpyrifos

Contact Info

Product

Resources

About