Acetylcholine Receptor Formation in Mouse–Chick Chimera

Auda-Boucher, Gwénola; Jarno, V.; Fournier-Thibault, Claire; Butler-Browne, Gillian; Fontaine-Pérus, Josiane

doi:10.1006/excr.1997.3706

Cited by 9 publications

(6 citation statements)

References 63 publications

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“…This may be related to the fact that at this age, most epaxial muscles are already innervated. Indeed, the timing of innervation of myotome-derived muscles in the chick precedes by ϳ2 d the establishment of neuromuscular contacts in limb muscles (Auda-Boucher et al, 1997). In contrast, at E8 in experimental embryos, BEN mRNA is still abundant in all fibers of epaxial muscles (Fig.…”

Section: Ben Expression In Embryonic Muscles Developing In Aneural Comentioning

confidence: 95%

“…A decrease in BEN expression is first observed in motor neurons of the medial motor column (Fig. 3), whose motor axons innervate epaxial muscles 2 d before the innervation of limb muscles by motor neurons of the lateral motor column (Auda-Boucher et al, 1997), in which a decrease of BEN expression is observed later during development (Fig. 3).…”

Section: Ben Expression Is Developmentally Regulated During Neuromuscmentioning

confidence: 99%

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BEN/SC1/DM-GRASP Expression during Neuromuscular Development: a Cell Adhesion Molecule Regulated by Innervation

et al. 1999

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BEN/SC1/DM-GRASP is a cell adhesion molecule belonging to the Ig superfamily that is transiently expressed during avian embryogenesis in a variety of cell types, including the motoneurons of the spinal cord. We have investigated the pattern of BEN expression during neuromuscular development of the chick. We show that both motoneurons and their target myoblasts express BEN during early embryonic development and that the protein becomes restricted at neuromuscular contacts as soon as postsynaptic acetylcholine receptor clusters are observed in muscle fibers. Muscle cells grown in vitro express and maintain BEN expression even when they fuse and give rise to mature myotubes. When embryos are deprived of innervation by neural tube ablation, BEN expression is observed in muscle fibers, whereas, in control, the protein is already restricted at neuromuscular synaptic sites. These results demonstrate that all myogenic cells intrinsically express BEN and maintain the protein in the absence of innervation. Conversely, when neurons are added to myogenic cultures, BEN is rapidly downregulated in muscle cells, demonstrating that innervation controls the restricted pattern of BEN expression seen in innervated muscles. After nerve section in postnatal muscles, BEN protein becomes again widely spread over muscle fibers. When denervated muscles are allowed to be reinnervated, the protein is reexpressed in regenerating motor axons, and reinnervation of synaptic sites leads to the concentration of BEN at neuromuscular junctions. Our results suggest that BEN cell adhesion molecule acts both in the formation of neuromuscular contacts during development and in the events leading to muscle reinnervation.

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Section: Ben Expression In Embryonic Muscles Developing In Aneural Comentioning

confidence: 95%

Section: Ben Expression Is Developmentally Regulated During Neuromuscmentioning

confidence: 99%

BEN/SC1/DM-GRASP Expression during Neuromuscular Development: a Cell Adhesion Molecule Regulated by Innervation

et al. 1999

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“…A final question is does the signal determining the timing of innervation originate from the nerve itself (e.g., stage of maturation) or is it inherent in each particular muscle group? Auda-Boucher et al (1997) grafted mouse somites into chick embryos, and examined the course of innervation in the grafted muscle. Normally, chick myotomally derived muscle expresses clustered AChR long before it is invaded by nerve, whereas the mouse epaxial musculature is first entered by the nerve, then later expresses AChR clusters.…”

Section: Innervation Of Myotomal Versus Migrating Musclesmentioning

confidence: 99%

The mammalian myotome: a muscle with no innervation

Deries

Collins

Duxson

2008

Evolution and Development

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The segmented muscular myotome is the first muscle to form in all vertebrates. In fish and amphibian embryos, the myotome becomes innervated very early and is essential for larval swimming. Its role in birds and mammals, however, is not clear. Using immunohistochemistry on sections and whole mounts of rat embryos, we demonstrate that the mammalian myotome differentiates and develops over a period of 3 days without being invaded by the outgrowing spinal nerves. In contrast, the limb muscle masses become filled with fine nerve branches from the first time that myocyte differentiation can be detected. Additionally, we show that the mammalian myotome does not express clustered acetylcholine receptors until after embryonic day 13.5, which corresponds to the beginning of its transformation into the adult epaxial muscles, showing that there are no functional myotomal neuromuscular junctions before this age. We suggest that the mammalian myotome has entirely lost the function of neurally controlled muscular contraction: its remaining functions are likely to be as a signaling tissue, as a structural scaffold, and as an incubator for myogenic precursors of the deep back, abdominal, and intercostal muscles.

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“…Although most studies have focussed on limb muscle development, the available studies on axial muscles suggests their development is very similar to that of limb muscles at these stages of development (e.g. [107,108]). …”

Section: Primary and Secondary Myogenesismentioning

confidence: 99%

Axial and limb muscle development: dialogue with the neighbourhood

Deries

Þorsteinsdóttir

2016

Cell. Mol. Life Sci.

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Skeletal muscles are part of the musculoskeletal system which also includes nerves, tendons, connective tissue, bones and blood vessels. Here we review the development of axial and limb muscles in amniotes within the context of their surrounding tissues in vivo. We highlight the reciprocal dialogue mediated by signalling factors between cells of these adjacent tissues and developing muscles and also demonstrate its importance from the onset of muscle cell differentiation well into foetal development. Early embryonic tissues secrete factors which are important regulators of myogenesis. However, later muscle development relies on other tissue collaborators, such as developing nerves and connective tissue, which are in turn influenced by the developing muscles themselves. We conclude that skeletal muscle development in vivo is a compelling example of the importance of reciprocal interactions between developing tissues for the complete and coordinated development of a functional system.

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Acetylcholine Receptor Formation in Mouse–Chick Chimera

Cited by 9 publications

References 63 publications

BEN/SC1/DM-GRASP Expression during Neuromuscular Development: a Cell Adhesion Molecule Regulated by Innervation

BEN/SC1/DM-GRASP Expression during Neuromuscular Development: a Cell Adhesion Molecule Regulated by Innervation

The mammalian myotome: a muscle with no innervation

Axial and limb muscle development: dialogue with the neighbourhood

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