The mammalian myotome: a muscle with no innervation

Deries, Marianne; Collins, Jennifer J. P.; Duxson, Marilyn J.

doi:10.1111/j.1525-142x.2008.00289.x

Cited by 22 publications

(20 citation statements)

References 44 publications

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“…This is of particular interest, because of the embryological origin of these muscles from the segmental myotome. During development in the rat (Rattus rattus), the TSP muscles arise by reorientation and elongation of the myotomal fibers, during which process adjacent myotomes blend together (Deries et al, 2008). Thus, it seemed likely that the longer fascicles of the TSP muscles might be constructed of fibers from multiple segments arranged in an end-to-end series.…”

Section: Histochemistrymentioning

confidence: 99%

“…This is an issue because the mouse is increasingly the model system of choice for developmental and genetic studies aimed at understanding the mammalian phenotype (Higuchi and Abe, 1987;Elliott and Sarver, 2004;Nelson and Nebert, 2004;Wang et al, 2005), therefore a thorough understanding of normal mouse morphology is essential for interpretation of abnormalities arising from genetic manipulations. Additionally, studies of the development of rodent epaxial muscles (e.g., Deries et al, 2008) have been hampered by a lack of precise information on their anatomy.…”

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confidence: 99%

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Morphology of the Lumbar Transversospinal Muscles Examined in a Mouse Bearing a Muscle Fiber‐Specific Nuclear Marker

Cornwall

Deries

Duxson

2010

The Anatomical Record

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Although the morphology of human lumbar transversospinal (TSP) muscles has been studied, little is known about the structure of these muscles in the mouse (Mus musculus). Such information is relevant given mice are often used as a ''normal'' phenotype for studies modeling human development. This study describes the gross morphology, muscle fiber arrangement, and innervation pattern of the mouse lumbar TSP muscles. A unique feature of the study is the use of a transgenic mouse line bearing a muscle-specific nuclear marker that allows clear delineation of muscle fiber and connective tissue boundaries. The lumbar TSP muscles of five mice were examined bilaterally; at each spinal level muscles attached to the caudal edge of the spinous process and passed caudally as a single complex unit. Fibers progressively terminated over the four vertebral segments caudad, with multiple points of muscle fiber attachment on each vertebra. Motor endplates, defined with acetylcholinesterase histochemistry, were consistently located half way along each muscle fiber, regardless of length, with all muscle fibers arranged in-parallel rather than in-series. These results provide information relevant to interpretation of developmental and functional studies involving this muscle group in the mouse and show mouse lumbar TSP muscles are different in form to descriptions of equivalent muscles in humans and horses. Anat Rec, 293:2107Rec, 293: -2113Rec, 293: , 2010. V V C 2010 Wiley-Liss, Inc.Key words: paravertebral muscles; lumbar; morphology; mouse; fiber arrangement This work examines the normal morphology of the transversospinal (TSP) muscles of the laboratory mouse (Mus musculus). The TSP muscles are a subgroup of the paravertebral muscles, located in the space between the spinous and transverse processes of the vertebral column, and existing throughout the spine between upper cervical spine and sacrum (Slijper, 1946). This group is described as consisting of individual muscles, including the semispinalis, multifidus, and rotatores. The paravertebral muscles and vertebral column receive significant scientific attention in clinical medicine, because of the prominence of back pain and spinal pathologies in humans (Boal and Gillette, 2004;Cassidy et al., 2005;Balague et al., 2007); however, our morphological understanding of these muscles is poor.Slijper (1946) studied the structure of paravertebral muscles in a wide range of large mammalian species, including the dugong (Dugong dugon) and armadillo (Dasypus). This muscle group has also been described in

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Section: Histochemistrymentioning

confidence: 99%

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confidence: 99%

Morphology of the Lumbar Transversospinal Muscles Examined in a Mouse Bearing a Muscle Fiber‐Specific Nuclear Marker

Cornwall

Deries

Duxson

2010

The Anatomical Record

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“…At a later stage, a new pool of proliferating muscle progenitors expressing Pax3 and Pax7 enter the myotome from the central dermomyotome Gros et al, 2005;Kassar-Duchossoy et al, 2005;Relaix et al, 2005), contributing to the growth of the myotome. Remarkably, in amniote embryos, the myotome is only a transient structure and does not get innervated (Deries et al, 2008). Rather, the segmented myotomes are transformed: epaxially into the complex deep back muscles and hypaxially into the intercostal and body wall muscles (Christ et al, 1983;Tremblay et al, 1998;Kalcheim et al, 1999;Deries et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

“…Whole-mount immunohistochemistry was performed as described in Deries et al, 2008. Briefly, embryos were fixed in 2% buffered paraformaldehyde, then washed and dehydrated in methanol.…”

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Extracellular matrix remodeling accompanies axial muscle development and morphogenesis in the mouse

Deries

Gonçalves

Vaz

et al. 2011

Developmental Dynamics

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Background: Skeletal myogenesis is extensively influenced by the surrounding environment. However, how the extracellular matrix (ECM) affects morphogenesis of muscles is not well understood. Results: We mapped the three-dimensional (3D) organization of fibronectin, tenascin, and laminin by immunofluorescence during early epaxial myogenesis in mouse embryos. We define four stages of dermomyotome/myotome development and reveal the 3D organization of myogenic cells within their ECM during those stages. Fibronectin is abundant in all interstitial tissues, while tenascin is restricted to intersegmental borders. Bundles of fibronectin and tenascin also penetrate into the myotome, possibly promoting myocyte alignment. A laminin matrix delineates the dermomyotome and myotome and undergoes dynamic changes, correlating with key developmental events. Conclusion: Our observations cast new light on how myotomal cells interact with their environment and suggest that, as the segmented myotomes transform into the epaxial muscle masses, the laminin matrix disassembles and myocytes use the abundant fibronectin matrix to reach their final organization. Developmental Dynamics 241:350-364,

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“…The protocol for whole mount immunostaining and imaging described below is an adaptation from previously reported methods used for other tissues [16][17][18][19] . Buffers and materials are described in Table 1 and Materials List.…”

Section: Whole-mount Immunofluorescence Staining and 3d Reconstructionmentioning

confidence: 99%

Human Dupuytren's <em>Ex Vivo</em> Culture for the Study of Myofibroblasts and Extracellular Matrix Interactions

Karkampouna

Kloen

Obdeijn

et al. 2015

JoVE

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Citation: Karkampouna, S., Kloen, P., Obdeijn, M.C., Riester, S.M., van Wijnen, A.J., Kruithof-de Julio, M. Human Dupuytren's Ex Vivo Culture for the Study of Myofibroblasts and Extracellular Matrix Interactions. J. Vis. Exp. (98), e52534, doi:10.3791/52534 (2015). AbstractOrgan fibrosis or "scarring" is known to account for a high death toll due to the extensive amount of disorders and organs affected (from cirrhosis to cardiovascular diseases). There is no effective treatment and the in vitro tools available do not mimic the in vivo situation rendering the progress of the out of control wound healing process still enigmatic.To date, 2D and 3D cultures of fibroblasts derived from DD patients are the main experimental models available. Primary cell cultures have many limitations; the fibroblasts derived from DD are altered by the culture conditions, lack cellular context and interactions, which are crucial for the development of fibrosis and weakly represent the derived tissue. Real-time PCR analysis of fibroblasts derived from control and DD samples show that little difference is detectable. 3D cultures of fibroblasts include addition of extracellular matrix that alters the native conditions of these cells.As a way to characterize the fibrotic, proliferative properties of these resection specimens we have developed a 3D culture system, using intact human resections of the nodule part of the cord. The system is based on transwell plates with an attached nitrocellulose membrane that allows contact of the tissue with the medium but not with the plastic, thus, preventing the alteration of the tissue. No collagen gel or other extracellular matrix protein substrate is required. The tissue resection specimens maintain their viability and proliferative properties for 7 days. This is the first "organ" culture system that allows human resection specimens from DD patients to be grown ex vivo and functionally tested, recapitulating the in vivo situation. Video LinkThe video component of this article can be found at

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The mammalian myotome: a muscle with no innervation

Cited by 22 publications

References 44 publications

Morphology of the Lumbar Transversospinal Muscles Examined in a Mouse Bearing a Muscle Fiber‐Specific Nuclear Marker

Morphology of the Lumbar Transversospinal Muscles Examined in a Mouse Bearing a Muscle Fiber‐Specific Nuclear Marker

Extracellular matrix remodeling accompanies axial muscle development and morphogenesis in the mouse

Human Dupuytren's <em>Ex Vivo</em> Culture for the Study of Myofibroblasts and Extracellular Matrix Interactions

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