Acetone–butanol–ethanol production from Kraft paper mill sludge by simultaneous saccharification and fermentation

Guan, Wenjian; Shi, Suan; Tu, Maobing; Lee, Yoon Y.

doi:10.1016/j.biortech.2015.10.102

Cited by 84 publications

(47 citation statements)

References 32 publications

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“…This is supported by the findings of Oguntimein and Moo-Young (1991), who reported the inhibitory effect of accumulated cellobiose and cellodextrin with a low degree of polymerization [49]. Moreover, the high solid loading also increases the viscosity of the slurry, and can result in poor mixing and mass transfer problem that reduce sugar conversion [50, 51]. …”

Section: Resultsmentioning

confidence: 54%

Characterization of a thermophilic cellulase from Geobacillus sp. HTA426, an efficient cellulase-producer on alkali pretreated of lignocellulosic biomass

et al. 2017

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A themophilic cellulase-producing bacterium was isolated from a hot spring district and identified as Geobacillus sp. HTA426. The cellulase enzyme produced by the Geobacillus sp. HTA426 was purified through ammonium sulfate precipitation and ion exchange chromatography, with the recovery yield and fold purification of 10.14% and 5.12, respectively. The purified cellulase has a molecular weight of 40 kDa. The optimum temperature and pH for carboxymethyl cellulase (CMCase) activity of the purified cellulase were 60°C and pH 7.0, respectively. The enzyme was also stable over a wide temperature range of 50°C to 70°C after 5 h of incubation. Moreover, the strain HTA426 was able to grow and produce cellulase on alkali-treated sugarcane bagasse, rice straw and water hyacinth as carbon sources. Enzymatic hydrolysis of sugarcane bagasse, which was regarded as the most effective carbon source for cellulase production (CMCase activity = 103.67 U/mL), followed by rice straw (74.70 U/mL) and water hyacinth (51.10 U/mL). This strain producing an efficient thermostable cellulose is a potential candidate for developing a more efficient and cost-effective process for converting lignocellulosic biomass into biofuel and other industrial process.

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Section: Resultsmentioning

confidence: 54%

Characterization of a thermophilic cellulase from Geobacillus sp. HTA426, an efficient cellulase-producer on alkali pretreated of lignocellulosic biomass

et al. 2017

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“…Ethanol production is based on the process of fermentation carried out by a variety of microorganisms such as fungi, bacteria, and yeasts. In ethanol production, simultaneous saccharification and fermentation (SSF) is considered to be more efficient and advantageous strategy of bioconversion compared to separate hydrolysis and fermentation (SHF) due to low cost, low end product inhibition, high yield and productivity [1,2, 3]. …”

Section: Introductionmentioning

confidence: 99%

“…The resistance of yeast to high temperature and ethanol concentration are desirable characteristics for production of bioethanol [10]. Thus, identifying or generating ethanol tolerant yeast strains could improve the final ethanol concentration and productivity, which in turn save energy on downstream ethanol recovery [11, 12, 3]. Yeast strains that show tolerance to stress imposed by high ethanol concentration are observed to have certain physiological properties which help them to survive such as intracellular accumulation of ergosterol, trehalose and proline [13, 14, 15].…”

Section: Introductionmentioning

confidence: 99%

Expression of C-5 sterol desaturase from an edible mushroom in fisson yeast enhances its ethanol and thermotolerance

Kamthan

Datta

2017

PLoS ONE

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Bioethanol is an environment friendly and renewable source of energy produced by the fermentation of agricultural raw material by a variety of microorganisms including yeast. Obtaining yeast strains that are tolerant to stresses like high levels of ethanol and high temperature is highly desirable as it reduces cost and increases yield during bioethanol production. Here, we report that heterologous expression of C-5 Sterol desaturase (FvC5SD)—an ergosterol biosynthesis enzyme from an edible mushroom Flammulina velutipes in fission yeast, not only imparts increased thermotolerance but also tolerance towards high ethanol concentration and low pH. This tolerance could be attributed to an increase of ≈1.5 fold in the level of ergosterol and oleic acid (C-18 unsaturated fatty acid) as analysed by gas chromatography- mass spectrometry. FvC5SD is a membrane localized iron binding enzyme that introduces double bond at C-5 position into the Δ7-sterol substrates to yield Δ5, 7- sterols as products. In F. velutipes, FvC5SD transcript was observed to be upregulated by ≈5 fold under low pH condition and by ≈ 9 folds and ≈5 fold at 40°C and 4°C respectively when compared to normal growth temperature of 23°C. Besides, susceptibility to cell wall inhibiting drugs like Congo red and Calcoflour white was also found to increase in FvC5SD expressing S. pombe strain. Alteration in membrane sterol and fatty acid composition could also lead to increase in susceptibility to cell wall inhibiting drugs. Thus, this study has immense industrial application and can be employed to ensure competitiveness of fermentation process.

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“…However, substrate cost remains a major factor influencing the economic viability of fermentative butanol production, accounting for up to 50% of the production cost (Green, 2011). Therefore, transition toward cheaper (non-edible) feedstocks offers the biggest opportunity for cost reduction and improved sustainability (Guan et al, 2016). Among the different types of biomass available, xylan, which is the second most abundant natural repository of xylose on Earth, is an attractive substrate for butanol production by microbial processes (Deutschmann and Dekker, 2012).…”

Section: Introductionmentioning

confidence: 99%

Simultaneous saccharification and fermentation of hemicellulose to butanol by a non-sporulating Clostridium species

2016

Bioresource Technology

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h i g h l i g h t sSimultaneous saccharification and fermentation of xylan to butanol by new strain MF28. Simultaneous fermentation of glucose and xylose to produce 11.9 g/L butanol. High yields of butanol on xylan and xylooligosaccharide were obtained. No acetone/ethanol byproducts were formed in converting lignocellulose to butanol. Non-sporulating strain MF28 is capable of continuous industrial-scale fermentation. a r t i c l e i n f o t r a c tProduction of lignocellulosic butanol has drawn increasing attention. However, currently few microorganisms can produce biofuels, particularly butanol, from lignocellulosic biomass via simultaneous saccharification and fermentation. Here we report discovery of a wild-type, mesophilic Clostridium sp. strain MF28 that ferments xylan to produce butanol (up to 3.2 g/L) without the addition of saccharolytic enzymes and without any chemical pretreatments. Application of selective pressure from 2-deoxy-Dglucose facilitated isolation of strain MF28, which exhibits inactivation of genes (gid and ccp genes) responsible for carbon catabolite repression, thus allowing strain MF28 to simultaneously ferment a combination of glucose (30 g/L), xylose (15 g/L), and arabinose (15 g/L) to produce 11.9 g/L of butanol. Strain MF28 possesses several unique features: (i) non-sporulating, (ii) no acetone/ethanol, (iii) complete hemicellulose-binding enzymatic domain, and (iv) absence of carbon catabolite repression. These unique characteristics demonstrate the industrial potential of strain MF28 for cost-effective biofuel generation from lignocellulosic biomass.

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Acetone–butanol–ethanol production from Kraft paper mill sludge by simultaneous saccharification and fermentation

Cited by 84 publications

References 32 publications

Characterization of a thermophilic cellulase from Geobacillus sp. HTA426, an efficient cellulase-producer on alkali pretreated of lignocellulosic biomass

Characterization of a thermophilic cellulase from Geobacillus sp. HTA426, an efficient cellulase-producer on alkali pretreated of lignocellulosic biomass

Expression of C-5 sterol desaturase from an edible mushroom in fisson yeast enhances its ethanol and thermotolerance

Simultaneous saccharification and fermentation of hemicellulose to butanol by a non-sporulating Clostridium species

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