Acetolactate synthase activity and chlorsulfuron sensitivity of gamma-irradiated lentil (<i>Lens culinaris</i> Medik.) cultivars

Malkawi, Hanan I.; AL-Quraan, Nisreen A.; Owais, W. M.

doi:10.1017/s0021859602002733

Cited by 9 publications

(10 citation statements)

References 27 publications

(44 reference statements)

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“…These mutagens can be effectively used to generate mutations in lentil that ultimately produces resistance in plants against herbicides. Many other studies reported that induced mutations are efficient in creation of herbicide resistant mutants in different crops [ 14 , 16 , 25 , 26 ].…”

Section: Discussionmentioning

confidence: 99%

“…Chant (2004) [ 26 ] developed an imidazolinone tolerant lentil mutant (RH44) by EMS mutagenesis. Malkawi et al ., (2003) [ 25 ] treated lentil cultivars with gamma radiations to develop tolerance against chlorsulfuron herbicide. Similarly, Sebastian and Chaleff (1987) [ 6 ] selected four mutants of soybean (1-126A, 1-166A, 1-183A and 1-184A) which appeared to be sufficiently tolerant to chlorsulfuron herbicide.…”

Section: Discussionmentioning

confidence: 99%

“…The seeds of selected herbicide resistant plants from the screening of M 2 generation were re-mutagenized at 150 Gy dose of gamma radiation followed by Malkawi et al [ 25 ]. Single plant progenies were sown in field and evaluated at 1.5X dose of respective herbicides.…”

Section: Methodsmentioning

confidence: 99%

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Pre-breeding of lentil (Lens culinaris Medik.) for herbicide resistance through seed mutagenesis

et al. 2017

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Lentil is a poor competitor of weeds and its sensitivity to herbicides is a major hurdle for large scale production. The present study was conducted to select herbicide resistant lentil genotypes through seed mutagenesis. Seeds of three advanced lentil genotypes (LPP 11001, LPP 11100 and LPP 11116) were treated with two different concentrations of ethyl methanesulfonate (EMS; 0.1 and 0.2%), hydrazine hydrate (HH; 0.02 and 0.03%) and sodium azide (SA; 0.01 and 0.02%) to develop M1 seed. The M2 was screened against two herbicides including Ally Max 28.6% SG (X = 34.58 g/ha and 1.5X = 51.87 g/ha) and Atlantis 3.6% WG (X = 395.2 g/ha and 1.5X = 592.8 g/ha) using the following three screening methods: post plant emergence (PPE), pre-plant incorporation (PPI) and seed priming (SP). Data were recorded on survival index and survival percentage from each experimental unit of every population. Plants in all populations were categorized following their reaction to herbicides. The newly developed populations showed greater variation for herbicide resistance when compared to their progenitors. Phenotypic traits were significantly reduced in all the screening environments. Overall, 671 herbicide resistant mutants were selected from all testing environments. The seeds from selected plants were re-mutagenized at 150 Gy of gamma radiation and evaluated against higher dose of herbicides. This allowed selection of 134 herbicide resistant mutants. The selected mutants are useful germplasm for herbicide resistance breeding of lentil.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Pre-breeding of lentil (Lens culinaris Medik.) for herbicide resistance through seed mutagenesis

et al. 2017

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“…After centrifugation, the GSH content in the supernatant of the maize root was measured spectrophotometrically at 412 nm in the presence of DTNB and calculated via a comparison with a known GSH concentration. 42 2.7 Determination of ALS activity ALS activity was determined according to the method of Malkawi et al 43 Acetolactate was measured by spectrophotometry (525 nm) and calculated by comparison against known acetolactate concentrations. Protein content was determined according to the Bradford method using bovine serum albumin as the reference material.…”

Section: Determination Of Gsh Contentmentioning

confidence: 99%

Protective efficacy of phenoxyacetyl oxazolidine derivatives as safeners against nicosulfuron toxicity in maize

Zhang

Gao

Hoang

et al. 2020

Pest Management Science

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BACKGROUND: Herbicide safeners mitigate crop damage without reducing herbicide efficacy. Here, the protective effects of phenoxyacetyl oxazolidine derivatives as potential safeners were evaluated with a view toward reducing injury caused by sulfonylurea herbicide nicosulfuron to sensitive maize varieties. RESULTS: Growth indices demonstrated that the bioactivity of compound 9 (N-phenoxyacety-2-methyl-2,4-diethyl-1,3-oxazolidine) was superior to that of R-28725 and all other compounds tested. Compound 9 induced endogenous glutathione and upregulated glutathione-S-transferase (GST) in maize. Thus, it could enhance maize tolerance to nicosulfuron. Compared with the untreated water control group, the maximum reaction rate of GST was increased by 37.62%, while the maximum velocity of GST was decreased by 61.93% after treatment with compound 9. Acetolactate synthase relative activity was significantly enhanced in the case of treatment with compound 9, indicating the excellent protective effects of compound 9 against nicosulfuron in maize. CONCLUSIONS: The present work demonstrates that phenoxyacetyl oxazolidine derivatives are potentially efficacious as herbicide safeners and merit further investigation.

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“…To investigate the effect of safener to target enzyme, ALS activity was determined as described previously (Malkawi et al, 2003). ALS activity was expressed as amount of acetoin composed from acetolactate catalyzed by ALS per hour per milligram of enzyme (nmol h -1 mg -1 protein).…”

Section: Determination Of Als Activitymentioning

confidence: 99%

3-Dichloroacetyl oxazolidine protect maize from imazethapyr herbicide injury

Zhao

et al. 2016

Chilean J. Agric. Res.

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Safeners are an important tool used to ensure the safe using of herbicide. The objective of this paper was to investigate the protective effect of four 3-dichloroacetyl oxazolidine safeners (3-dichloroacetyl-2,2-dimethyl-1,3-oxazolidine [R-28725], racemate of 3-dichloroacetyl-2,2-dimethyl-4-ethyl-1,3-oxazolidine, and its two chiral stereoisomers) in reducing the injury caused by imazethapyr. Physiological and biochemical tests were conducted under laboratory condition, by using seed treatment with safeners and soil treatment with imazethapyr, respectively. The interaction of two safeners (R-28725 and R-stereoisomer) and imazethapyr reduced the injury of maize significantly, and also increased glutathione content, activity of glutathione S-transferases, and activity of acetolactate synthase in maize. When induced by R-stereoisomer, the GSH content in root and in shoot increased 100.7% and 73.6%, respectively. When induced by R-28725, the GST activity in vivo increased threefold and the GST activity in vitro more than doubled. The kinetic parameter Vmax of GST in the maize treated with R-28725 and R-stereoisomer increased by 102.2% and 81.9%, respectively, compared with the control. The results also showed that R-28725 and R-stereoisomer induced glutathione S-transferases affinity for the substrate of conjugation reaction significantly.

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Acetolactate synthase activity and chlorsulfuron sensitivity of gamma-irradiated lentil (Lens culinaris Medik.) cultivars

Cited by 9 publications

References 27 publications

Pre-breeding of lentil (Lens culinaris Medik.) for herbicide resistance through seed mutagenesis

Pre-breeding of lentil (Lens culinaris Medik.) for herbicide resistance through seed mutagenesis

Protective efficacy of phenoxyacetyl oxazolidine derivatives as safeners against nicosulfuron toxicity in maize

3-Dichloroacetyl oxazolidine protect maize from imazethapyr herbicide injury

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