Acetaminophen and Metamizole Induce Apoptosis in HT 29 and SW 480 Colon Carcinoma Cell Lines In Vitro

Bundscherer, Anika; Malsy, Manuela; Gruber, Michael; Graf, Bernhard M.; Sinner, Barbara

doi:10.21873/anticanres.12280

Cited by 8 publications

(3 citation statements)

References 25 publications

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“…Adherent cells were rinsed with PBS (37˚C) (Sigma-Aldrich) and detached by standard trypsinization. Fluorescein isothiocyanate Annexin V Apoptosis detection Kit (BD, Heidelberg, Germany) was used according to the manufacturer's protocol as described previously, PI staining was used for the exclusion of necrotic cells (10). All tests were performed in duplicates as at least three independent experiments.…”

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confidence: 99%

Effects of Volatile Anesthetics on Proliferation and Viability of SW480 Colon Cancer Cells In Vitro

et al. 2019

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Background/Aim: For patients undergoing cancer surgery, the risk for cancer progression is enhanced during the perioperative period. To what extent the type of anesthetic can affect the metastatic process and finally the outcome of patients with cancer is under debate. For this reason, the aim of this study was to investigate the effects of the volatile anesthetics sevoflurane and desflurane on colon cancer cells in vitro. Materials and Methods: SW480 colon carcinoma cells were exposed for 3 or 6 h to sevoflurane (1 or 2.5 vol%) or desflurane (6 or 12 vol%). Cell cycle distribution was analyzed by flow cytometry after a 24-72 h recovery and apoptosis was detected by annexin V staining after a 0-48 h recovery. Viability was tested by measuring ATP content after 0 and 24 h recovery. Results: Treatment with sevoflurane or desflurane caused no or only slight changes in cell-cycle distribution and apoptosis rate. Desflurane at 12vol% significantly reduced cell viability by 17±25% and 11±22% after 3 and 6 h incubation and 24 h recovery, respectively, while 2.5 vol% sevoflurane slightly increased viability. Conclusion: At clinically relevant concentrations, sevoflurane and desflurane had only slight effects on SW480 colon cancer cells in vitro.

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confidence: 99%

Effects of Volatile Anesthetics on Proliferation and Viability of SW480 Colon Cancer Cells In Vitro

et al. 2019

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“…Indomethacin reduced the invasion capacity of breast cancer cells, most likely due to changes in the choline-phospholipid and triacylglycerol metabolism (21). In previous studies acetaminophen and metamizole revealed proapoptotic effects in colon cancer and antiproliferative effects in pancreatic cancer cells (22). Through increased expression of the differentiation markers, paracetamol seems to be able to change breast cancer cells into a more benign type marked by reduced tumour growth, low invasion capacity and increased sensitivity to anti-tumour agents (23).…”

Section: Discussionmentioning

confidence: 94%

The Effects of Analgesics on the Migration of Pancreatic Cancer Cells

Malsy

Hackl²,

Graf

et al. 2022

In Vivo

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Background/Aim: Adenocarcinoma of the pancreas is one of the most aggressive malignant diseases in humans. Characteristics of this tumour type are poor response to radiotherapy and chemotherapeutic agents as well as metastasis in the absence of an organ capsule. The best therapeutic option is surgical removal of the tumour followed by chemotherapy or radiotherapy. Yet, even after surgical R0resection, the 5-year survival probability is only about 20% because of the high recurrence rate of this tumour and complications due to metastases. Furthermore, recent studies have shown that the perioperative period is a particularly vulnerable phase, during which tumour progression and metastasis may be facilitated. The effects of analgesics administered during the perioperative period are still unknown. The present work investigated the effects of analgesics on pancreatic cancer cell migration in vitro. Materials and Methods: The migratory potential of pancreatic cancer cells was analysed using a Cell Migration Assay Kit with a Boyden chamber, in which cells migrate through a semi-permeable membrane under different stimuli. Cell concentration was measured by reading fluorescence (Ex/Em=530/590 nm) in a plate reader. Results: Migration in PANC-1 pancreatic cancer cells was significantly decreased after 24 h stimulation with 100 μM of ropivacaine, 100 nM of sufentanil, 1,000 μM of ropivacaine and 1,000 nM of sufentanil. In the PaTu 8988t cell line, incubation with 10 μM of ropivacaine caused a slight but statistically significant increase in migration, whereas 576 This article is freely accessible online.

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“…However, the results obtained with animal tissues may be different from those obtained with human tissue due to differences in sensitivity (18). In numerous studies where animal tissues were not used, commercial cell lines were used (19,20). However, in systems such as cell lines, one type of cell is used, and in such commercial cell lines, no complex coordination mechanisms with the microenvironment involving other tumor-associated cells are present.…”

Section: Discussionmentioning

confidence: 99%

Investigation of the effect of dipyrone on cells isolated from intervertebral disc tissue

et al. 2019

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The present study aimed to evaluate the effects of dipyrone, an indispensable analgesic, anti-pyretic and anti-spasmodic used in emergency departments, on nucleus pulposus and annulus fibrosus cells in vitro . After surgical biopsy, primary cell cultures were prepared from intact intervertebral disc tissues. Dipyrone was administered to the cultures in the experimental groups except for the control group. The data obtained were statistically evaluated. The proliferation was identified to be suppressed via MTT analysis. The gene expression profile of the intervertebral disc cells in the dipyrone-treated groups was significantly changed. The expression of chondroadherin, cartilage oligo matrix protein, interleukin-1β and metalloproteinase (MMP)-19 genes were decreased, but MMP-13 and MMP-7 genes expressions were increased, as determined via reverse transcription-quantitative PCR. AO/PI staining revealed that no apoptotic or other type of cell death was detectable after administration of dipyrone does not mean that the drug is innocuous. The occurrence of cellular senescence and/or the halt of cell proliferation may also be important mechanisms underlying the adverse inhibitory effects of dipyrone. Therefore, prior to administering dipyrone in clinical practice, all possible adverse effects of this drug should be considered.

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Acetaminophen and Metamizole Induce Apoptosis in HT 29 and SW 480 Colon Carcinoma Cell Lines In Vitro

Abstract: In clinically relevant doses, acetaminophen and/or metamizole induce apoptosis in both colon cancer cell lines. Both mitochondrial and death receptor pathways might be involved in acetaminophen-induced apoptosis.

Cited by 8 publications

References 25 publications

Effects of Volatile Anesthetics on Proliferation and Viability of SW480 Colon Cancer Cells In Vitro

Effects of Volatile Anesthetics on Proliferation and Viability of SW480 Colon Cancer Cells In Vitro

The Effects of Analgesics on the Migration of Pancreatic Cancer Cells

Investigation of the effect of dipyrone on cells isolated from intervertebral disc tissue

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