ACE2 and LOX Enzyme Inhibitions of Different Lavender Essential Oils and Major Components Linalool and Camphor

Biltekin, Sevde Nur; Karadağ, Ayşe Esra; Demırcı, Betül; Demirci, Fatih

doi:10.1021/acsomega.2c04518

Cited by 19 publications

(19 citation statements)

References 45 publications

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“…Furthermore, 3,5- O -dicaffeoylquinic acid, another phenolic compound abundant in the prepared H. italicum extracts [ 10 ], is also a potent 5-LOX inhibitor [ 80 ]. Finally, according to previous studies, essential oil components, such as linalool, may also contribute to the anti-LOX activity [ 81 ].…”

Section: Resultsmentioning

confidence: 99%

Hydroxypropyl-β-Cyclodextrin-Based Helichrysum italicum Extracts: Antioxidant and Cosmeceutical Activity and Biocompatibility

et al. 2023

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Two Helichrysum italicum extracts, OPT-1 (rich in phenolic acids) and OPT-2 (rich in total phenols and flavonoids), were prepared using hydroxypropyl-β-cyclodextrin (HP-β-CD)-assisted extraction. The prepared extracts were rich in phenolic compounds, including flavonoids and phenolic acids. GC-MS analysis of the extracts identified neryl acetate, neo-intermedeol, β-selinene, γ-curcumene, italidione I, and nerol as the main volatile components of the extracts, as well as plant sterols, γ-sitosterol, campesterol, and stigmasterol. The antioxidant (DPPH radical scavenging, reducing power, and a carotene linoleic acid assay) and cosmeceutical (anti-hyaluronidase, anti-tyrosinase, anti-lipoxygenase, ovalbumin anti-coagulation, and a UV-absorption assay) activity of the extracts in most of the assays was better than the activity of the applied positive controls. Especially low were the IC50 values of the extracts in the anti-hyaluronidase (14.31 ± 0.29 μL extract/mL and 19.82 ± 1.53 μL extract/mL for OPT-1 and OPT-2, respectively) and the anti-lipoxygenase (0.96 ± 0.11 μL extract/mL and 1.07 ± 0.01 μL extract/mL for OPT-1 and OPT-2, respectively) assays. The extracts were non-toxic to HaCaT cells in concentrations of up to 62.5 µL extract/mL assuring their status as excellent candidates for cosmeceutical product development appropriate for direct use in cosmetic products without solvent evaporation.

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Section: Resultsmentioning

confidence: 99%

Hydroxypropyl-β-Cyclodextrin-Based Helichrysum italicum Extracts: Antioxidant and Cosmeceutical Activity and Biocompatibility

et al. 2023

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“…The literature reports supported these findings concerning other medicinal plants. Indeed, several studies have reported the chemical composition of LSEO, with some indicating that, in addition to the fenchone/camphor chemotypes, the chemical compositions of LSEO collected in Morocco and Greece contained 1,8-cineole and camphene, and α-cardinol, respectively, while others disclosed the presence of myrtenyl acetate, bornyl acetate, linalyl acetate, camphene, linalool, borneol, γ-terpinene, lavandulyl acetate, and caryophyllene as major compounds [ 10 , 18 , 43 , 44 , 45 , 46 ]. Besides the presence of some main compounds, our study clearly revealed the chemical composition quantitative and qualitative variability of L. stoechas plants collected from three different regions.…”

Section: Resultsmentioning

confidence: 99%

Chemical Profiling and Biological Properties of Essential Oils of Lavandula stoechas L. Collected from Three Moroccan Sites: In Vitro and In Silico Investigations

Benali

Lemhadri

Harboul

et al. 2023

Plants

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The aim of this study was the determination of the chemical compounds of Lavandula stoechas essential oil from Aknol (LSEOA), Khenifra (LSEOK), and Beni Mellal (LSEOB), and the in vitro investigation of their antibacterial, anticandidal, and antioxidant effects, and in silico anti-SARS-CoV-2 activity. The chemical profile of LSEO was determined using GC-MS-MS analysis, the results of which showed a qualitative and quantitative variation in the chemical composition of volatile compounds including L-fenchone, cubebol, camphor, bornyl acetate, and τ-muurolol; indicating that the biosynthesis of essential oils of Lavandula stoechas (LSEO) varied depending on the site of growth. The antioxidant activity was evaluated using the ABTS and FRAP methods, our results showed that this tested oil is endowed with an ABTS inhibitory effect and an important reducing power which varies between 4.82 ± 1.52 and 15.73 ± 3.26 mg EAA/g extract. The results of antibacterial activity of LSEOA, LSEOK and LSEOB, tested against Gram-positive and Gram-negative bacteria, revealed that B. subtilis (20.66 ± 1.15–25 ± 4.35 mm), P. mirabilis (18.66 ± 1.15–18.66 ± 1.15 mm), and P. aeruginosa (13.33 ± 1.15–19 ± 1.00 mm) are the most susceptible strains to LSEOA, LSEOK and LSEOB of which LSEOB exhibits bactericidal effect against P. mirabilis. furthermore The LSEO exhibited varying degrees of anticandidal activity with an inhibition zones of 25.33 ± 0.5, 22.66 ± 2.51, and 19 ± 1 mm for LSEOK, LSEOB, and LSEOA, respectively. Additionally, the in silico molecular docking process, performed using Chimera Vina and Surflex-Dock programs, indicated that LSEO could inhibit SARS-CoV-2. These important biological properties of LSEO qualify this plant as an interesting source of natural bioactive compounds with medicinal actions.

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“…Experiments were performed in triplicate, and the average results were reported as previously. 32 3.7. p38MAPK Enzyme Inhibitory Assay. The ELISA Kit (Invitrogen) was applied in accordance with the manufacturer's instructions.…”

Section: Methodsmentioning

confidence: 99%

“…Nordihydroguaiaretic acid (NDGA) was used as a positive control. Experiments were performed in triplicate, and the average results were reported as previously …”

Section: Methodsmentioning

confidence: 99%

In Vitro Anti-Inflammatory and Anticancer Evaluation of Mentha spicata L. and Matricaria chamomilla L. Essential Oils

et al. 2023

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Spearmint, Mentha spicata L., and the German chamomile, Matricaria chamomilla L., preparations are used against inflammatory conditions traditionally and in modern medicinal applications. This present study aimed to evaluate pharma-grade essential oils for their in vitro anti-inflammatory and anticancer effects using COX-1, COX-2, and 5-LOX enzyme assays, as well as their apoptosis potential through the caspase pathway. In addition, the (3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide) (MTT) assay was applied to evaluate the in vitro cytotoxic effects using HEK293/A549, MCF7, and PC3 cell lines. Major components of M. spicata essential oil were confirmed both by gas chromatography (GC)-flame ionization detector (FID) and GC/mass spectrometry (MS) as 72.8% carvone, 12.6% limonene, 2.2% 1,8-cineole, 1.3% myrcene, and 1% trans-dihydrocarvone. The major components of M. chamomilla essential oil were also confirmed as 47.9% α-bisabolol oxide A, 16.8% α-bisabolol, 13.8%, (Z)-β-farnesene, 5.8% α-bisabolol oxide, and 4.7% α-bisabolene oxide A. The IC50 values for M. chamomilla essential oil on A549, MCF7, PC3, and HEK293 cells were calculated as 208.54 ± 1.39, 315.44 ± 1.17, 197.52 ± 0.98, and 638.79 ± 1.15 μg/mL, respectively, whereas the IC50 values for M. spicata essential oil on A549, MCF7, and PC3 cells were 672.13 ± 2.57, 708.27 ± 2.05, and 206.49 ± 1.48 μg/mL, respectively. For M. spicata essential oil, no cytotoxic effects on healthy HEK293 cells were observed at the tested concentrations. The essential oils increased the apoptotic activity, where all results were statistically significant. According to the anti-inflammatory evaluation, both M. chamomilla and M. spicata oils showed selective COX-2 inhibitions, where the SI values were calculated as 0.30 and 0.67, respectively. Overall, both M. spicata and M. chamomilla essential oils showed selective inhibition on the COX-2 enzyme and apoptosis against the selected cancer cell lines for the first time, to the best of our knowledge, with this specific dual mode of action. The initial results encourage further detailed in vivo experimental evaluations.

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ACE2 and LOX Enzyme Inhibitions of Different Lavender Essential Oils and Major Components Linalool and Camphor

Cited by 19 publications

References 45 publications

Hydroxypropyl-β-Cyclodextrin-Based Helichrysum italicum Extracts: Antioxidant and Cosmeceutical Activity and Biocompatibility

Hydroxypropyl-β-Cyclodextrin-Based Helichrysum italicum Extracts: Antioxidant and Cosmeceutical Activity and Biocompatibility

Chemical Profiling and Biological Properties of Essential Oils of Lavandula stoechas L. Collected from Three Moroccan Sites: In Vitro and In Silico Investigations

In Vitro Anti-Inflammatory and Anticancer Evaluation of Mentha spicata L. and Matricaria chamomilla L. Essential Oils

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