ACE inhibition by astilbin isolated from Erythroxylum gonocladum (Mart.) O.E. Schulz

Abstract

Erythroxylum species have several traditional uses in different countries, including the treatment of hypertension. The ethanol extract from E. gonocladum aerial parts, a species endemic to the Brazilian cerrado, elicited a concentration-dependent inhibition of angiotensin converting enzyme (ACE) (pIC(50)=4.53+/-0.05). Extract fractionation led to the isolation of two compounds, whose structures were assigned by spectrometric data as astilbin and beta-sitosterol, along with a mixture of palmitic, stearic and linolenic acids. This is the first report on the occurrence of these compounds on E. gonocladum. Astilbin promoted significant ACE inhibition in vitro (pIC(50)=5.86+/-0.33) and its activity did not differ from captopril, when both compounds were assayed at 10 microM concentration.

The Cyclitol L-(+)-Bornesitol as an Active Marker for the Cardiovascular Activity of the Brazilian Medicinal Plant <i>Hancornia speciosa</i>

“…Serum Nitrite Determination The nitrite (NO 2 − ) concentration was measured using the Griess reaction. 23) An aliquot (100 µL) of plasma was added to a microtiter plate and the enzymatic treatment was started by adding 100 µL of Griess reagent [0.1% (w/v) N-(1-naphthyl) ethylenediamine and 1.0% (w/v) sulphanilamide in 2.5% (v/v) phosphoric acid] for 10 min at room temperature and sheltered from light. The absorbance of each sample was determined at 545 nm, and total nitrite concentrations were calculated from the slope of the standard curves established using known concentrations of nitrite.…”

Hancornia speciosa Gomes induces hypotensive effect through inhibition of ACE and increase on NO

“…ACE inhibitory activity was determined using a method described by Lucas-Filho et al (2010), modified to employ mice plasma as enzyme source. Mice received 1 mg/kg SFH, 10 mg/kg captopril or saline by intragastric gavage.…”

Review of angiotensin-converting enzyme inhibitory assay: Rapid method in drug discovery of herbal plants

“…[65] The astilbin (with IC 50 of 4.53 mg/mL) were isolated from Erythroxylum gonocladum plant. [56] The quercetin isolated from Peperomia pellucida L. Kunth herbs with IC 50 of 7.72 mg/mL. [63] By knowing the active compounds that play a role as an ACE inhibitor, the researchers of natural products are more focused on enrichment, dereplication, and isolation the active compound that can be applied in the in vivo assay method and further development.…”