Accuracy of reverse‐transcription polymerase chain reaction and loop‐mediated isothermal amplification in diagnosing severe fever with thrombocytopenia syndrome: A systematic review and meta‐analysis

Wen, Tian; Ren, Xiaotun; Gao, Xu; Zhang, Yuanyuan; Chen, Zhihai; Zhang, Wei

doi:10.1002/jmv.28068

Cited by 3 publications

(3 citation statements)

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“…17 Previous studies have evaluated the detection of SFTS using reverse transcription LAMP (RT-LAMP) and reported the sensitivity of 84.9%-99% [16][17][18][19] and specificity as high as approximately 99% (95% confidence interval: 0.93-1.00). 20 These studies suggest that RT-LAMP as a viable detection method with a wide range of clinical applications. However, in clinical practice, the relationship between the application procedures of RT-LAMP in the disease process has not been evaluated, and results of RT-LAMP have not been compared among samples collected from patients during different periods.…”

Section: Several Methods Have Been Developed For the Diagnosis Of Sftsmentioning

confidence: 95%

“…The results of LAMP can be determined by observing the change in the color of mixture with the naked eye, eliminating the need for highly trained technicians 17 . Previous studies have evaluated the detection of SFTS using reverse transcription LAMP (RT‐LAMP) and reported the sensitivity of 84.9%–99% 16–19 and specificity as high as approximately 99% (95% confidence interval: 0.93–1.00) 20 …”

Section: Introductionmentioning

confidence: 99%

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Evaluation of reverse transcription loop‐mediated isothermal amplification assay for the detection of severe fever with thrombocytopenia syndrome in clinical laboratories: A single‐center study

Tian,

Zhang,

Geng

et al. 2023

Journal of Medical Virology

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Severe fever with thrombocytopenia syndrome (SFTS) is an acute infectious disease prevalent in East Asia with a high mortality rate (5%–30%). Reverse transcription loop‐mediated isothermal amplification (RT‐LAMP), a rapid nucleic acid‐based diagnostic technique, is a useful alternative for the clinical diagnosis of SFTS, particularly in resource‐limited hospitals or rural clinics in SFTS virus‐endemic regions. However, the actual clinical sensitivity and specificity of RT‐LAMP remain unclear. This study evaluated the field application of RT‐LAMP. This prospective field study included 130 patients with laboratory‐confirmed SFTS from Yantai, Shandong Province, China. Two sets of RT‐LAMP primers were validated, and one set of RT‐LAMP assays was optimized for field detection. Nucleic acids of serially collected serum/plasma samples were identified using quantitative reverse transcription polymerase chain reaction (RT‐qPCR) and RT‐LAMP. In laboratory tests, we optimized the detection time of primer set 2 for the RT‐LAMP to 60 min. Notably, the onsite testing of 279 plasma samples from patients with SFTS revealed that the sensitivity and specificity of the test were 81.9% and 96.3%, respectively. We also analyzed samples with different durations of the disease, and our study showed that the sensitivity of RT‐LAMP detection at the beginning of admission was 89.92%. Univariate analysis showed that the detection rate of RT‐LAMP was similar to that of RT‐qPCR in the first 5 days of the disease course and was lower than that of RT‐qPCR on Days 6 and 14–15 of the disease course. The positive detection rate in patients aged ≥ 65 years was significantly higher than that in younger age groups. RT‐LAMP is a simple, suitable, and rapid clinical detection method of SFTS onsite screening. It is more suitable for screening patients in the early stages of the disease and analyzing samples obtained from patients aged ≥ 65 years before the 6th day of the disease course.

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Section: Several Methods Have Been Developed For the Diagnosis Of Sftsmentioning

confidence: 95%

Section: Introductionmentioning

confidence: 99%

Evaluation of reverse transcription loop‐mediated isothermal amplification assay for the detection of severe fever with thrombocytopenia syndrome in clinical laboratories: A single‐center study

Tian,

Zhang,

Geng

et al. 2023

Journal of Medical Virology

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“…Detection of SFTSV genome could be achieved by different nucleic acid detection techniques such as RT-PCR (13,(40)(41)(42)(43), real-time RT-PCR (23,28,41,(44)(45)(46)(47)(48), LAMP (24,(49)(50), as well as RPA (25,(51)(52).…”

Section: Nucleic Acid Detectionmentioning

confidence: 99%

Advancements in the Worldwide Detection of Severe Fever with Thrombocytopenia Syndrome Virus Infection from 2009 to 2023

Lin¹,

Wang²,

Teng³

2023

China CDC Weekly

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Severe fever with thrombocytopenia syndrome (SFTS) is a growing concern as an emerging tick-borne infectious disease originating from the SFTS virus (SFTSV), a recent addition to the Phlebovirus genus under the family of bunyaviruses. SFTS is typically identified by symptoms such as fever, thrombocytopenia, leukopenia, and gastrointestinal problems, accompanied by a potentially high case fatality rate. Thus, early and accurate diagnosis is essential for effective treatment and disease management. This review delves into the existing methodologies for SFTS detection, including pathogenic, molecular, and immunological technologies. PATHOGENIC CHARACTERISTICS OF SFTSVStructural and Genetic Analysis of SFTSV SFTSV, a negative-sense RNA virus from the China CDC Weekly Chinese Center for Disease Control and Prevention CCDC Weekly / Vol. 5 / No. 31

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