Accuracy of Raman spectroscopy in discrimination of nasopharyngeal carcinoma from normal samples: a systematic review and meta-analysis

Li, Yuan; Su, Sihui; Zhang, Yingzhe; Liu, Shiyao; Jin, Hongyu; Zeng, Qianqing; Cheng, Lei

doi:10.1007/s00432-019-02934-y

Cited by 8 publications

(3 citation statements)

References 41 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Raman spectroscopy is a label-free and non-destructive approach that provides intrinsic molecular profile of a single cell. Single-cell Raman Spectra (SCRS) is widely used in biomedical field, for instance, discriminating cancer cells from the normal ones [4], tracking the drug biding sites, analyse the components in cancer cells, monitoring anticancer drug treatment [5], et al The combination of stable isotope probing and Raman spectroscopy can reveal more information on cell metabolism [6]. Among all the isotopes, deuterium is the one with universality as H 2 O is widely involved in cell metabolism, such as the synthesis of lipids, fatty acids, proteins, and nucleic acids.…”

Section: Introductionmentioning

confidence: 99%

Raman spectroscopy and machine learning for the classification of breast cancers

Zhang

Peng³

et al. 2022

Spectrochimica Acta Part A: Molecular and Biomolecular Spectros

View full text Add to dashboard Cite

Section: Introductionmentioning

confidence: 99%

Raman spectroscopy and machine learning for the classification of breast cancers

Zhang

Peng³

et al. 2022

Spectrochimica Acta Part A: Molecular and Biomolecular Spectros

View full text Add to dashboard Cite

“…Raman spectroscopy is a label‐free and non‐destructive approach that provides an intrinsic molecular profile of a single cell. Single‐cell Raman Spectroscopy (SCRS) is widely used in the biomedical field, for instance, to discriminate cancer cells from the normal ones, [ 6 ] track the drug biding sites, analyze the components of cancer cells, and monitor anti‐cancer drug treatment. [ 7 ] Stable isotope probing is a useful method for monitoring biosynthesis.…”

Section: Introductionmentioning

confidence: 99%

The Raman Deuterium Isotope Probing method as a new assay for evaluating the cytotoxicity level of the GSK2334470 to the MCF‐7 cells

Huang

et al. 2023

J Raman Spectroscopy

View full text Add to dashboard Cite

The Raman Deuterium Isotope Probing (Raman-DIP) method was applied to study the response of human breast adenocarcinoma cells (the MCF-7 cells) to the GSK2334470 drug in vitro. The cytotoxicity level of the GSK2334470 to the MCF-7 cell evaluated by the Raman-DIP method and CCK-8 assay was highly matched, as both methods suggested that 10 μM was the cytotoxic concentration for inhibition of cell regeneration. In additional, the relationship between drug dose and the cell response (RSI) were fitted, showing that our experimental data were consistent with the dose-response relationship, and the Raman-DIP could be applied for sensitivity testing of targeted anti-cancer drugs. The variation of the bio-macromolecules reflected by the Raman signal in the fingerprint range was closely related to the GSK2334470 concentration. The Raman-DIP method can monitor the inhibition of the GSK2334470 to the MCF-7 cell proliferation with high accuracy as the CCK-8 assay. The combination of Raman-DIP results and fingerprint range information can effectively improve the evaluation, serving as a supplement for the existing anti-cancer drug sensibility assay. This method is valuable to reveal the intracellular macromolecules changes related to the drug treatment. K E Y W O R D Santi-cancer drug screening, breast cancer, deuterium isotope probing, Raman spectroscopy, single cell level Xia Huang and Chengjian Li contributed equally to this work and should be considered co-first authors.

show abstract

“…The use of Raman spectroscopy for the analysis of biological specimens has significantly increased during the past few years. Raman spectroscopy has been extensively used in clinical diagnostics of cancer (5) including breast cancer (6)(7)(8)(9)(10)(11)(12) , lung cancer (13) , ovarian cancer (14,15) , cervical cancer (16)(17)(18) , oral cancer (19) , nasopharyngeal carcinomas (20) , brain tumors (21)(22)(23) , Gastrointestinal Tumors (24,25) , chondrogenic tumors (26) , prostate cancer (27) , colorectal cancer (28,29) , skin cancer (30) , lymphoma (31) . Moreover, Raman spectroscopy has been utilized as an emerging tool for diagnostics of various infectious diseases (32) .…”

Section: Introductionmentioning

confidence: 99%

Bioethical Education and Standardization of Sample Handling Procedures in Raman Spectroscopy Research Studies Involving Human Subjects

Jain¹,

Verma²,

Chougule³

et al. 2022

IJST

View full text Add to dashboard Cite

Novelty: Raman spectroscopy is extensively explored for the disease diagnostics in recent decade. There is limited literature available concerning the standardization of sample handling procedures in Raman spectroscopy research studies involving human subjects. In fact, the present study provided guidance for a harmonize data to conduct Raman spectroscopy research studies involving human subjects for better outcomes based on ethical principles. Objectives: Globally, multi-disciplinary research is conducted for better outcomes in the welfare of humankind. The present study was aimed to provide basic guidance of bioethical education for research students conducting research involving human subjects. Further, the present study was attempted to standardize sample handling procedure in Raman spectroscopy research involving human subjects for better outcomes in disease diagnosis. Materials and methods:We have provided bioethical recommendations based on fundamental ethics codes. The standardization of sample handling procedure was developed using human surgical samples of breast cancer patients. Findings: The researcher should justify the inclusion and exclusion criteria for biological samples to conduct the scientifically valid research study. Research studies involving biological samples shall develop research protocol for the preparation and handling of biological samples. The results of present study suggest that fresh clinically unprocessed tissue samples are superior to conduct research studies involving Raman spectroscopy for disease progression. In case the research is not concerned with positive net benefit for human participants with diagnosis of communicable diseases, the researcher shall exclude these patients from the research study. Informed consent, preferably in writing, has to be obtained from participants in a language in that participant comprehend. Conclusion: The present study described primary bioethical education and standardization techniques for research students to plan and conduct Raman spectroscopy research studies involving human biological materials. https://www.indjst.org/

show abstract

Accuracy of Raman spectroscopy in discrimination of nasopharyngeal carcinoma from normal samples: a systematic review and meta-analysis

Cited by 8 publications

References 41 publications

Raman spectroscopy and machine learning for the classification of breast cancers

Raman spectroscopy and machine learning for the classification of breast cancers

The Raman Deuterium Isotope Probing method as a new assay for evaluating the cytotoxicity level of the GSK2334470 to the MCF‐7 cells

Bioethical Education and Standardization of Sample Handling Procedures in Raman Spectroscopy Research Studies Involving Human Subjects

Contact Info

Product

Resources

About