A statically determinate kinematic mount structure is designed for an astronomical observation instrument. The basic principle of the proposed kinematic mount is introduced in detail, including the design principle, its structure, and its degrees of freedom. The compliance equations for the single-axis right circle flexure hinge are deduced, and mathematical models of the compliances of the bipod in the X-axis and Z-axis directions are established. Based on the index requirements, the range of one design parameter (the hinge groove depth, R) for the kinematic mount is determined. Parametric design is performed, with the entire structure being the design object and the first three eigenfrequencies as the design objective; the final design parameter for the kinematic mount is 1.9 mm. The first three eigenfrequencies of the final structure are 36.49 Hz, 38.65 Hz, and 72.41 Hz, which meet the frequency requirements. The Z-direction deformation and the bipod compliances in the X-axis and Z-axis directions are analyzed through simulations and experiments. The results show that (1) the Z-direction deformation of the bipod meets the displacement requirement; (2) the deviations between the finite element results and the compliance equation C results, and between the finite element results and the compliance equation C results are 8.8% and 3.92%, respectively; (3) the deviation between the experimental results and the compliance equation C results is 10.3%. It is concluded that the bipod compliance equations in the X-axis and Z-axis directions are valid, and that the kinematic mount thus meets the design requirements.
The Raman Deuterium Isotope Probing (Raman-DIP) method was applied to study the response of human breast adenocarcinoma cells (the MCF-7 cells) to the GSK2334470 drug in vitro. The cytotoxicity level of the GSK2334470 to the MCF-7 cell evaluated by the Raman-DIP method and CCK-8 assay was highly matched, as both methods suggested that 10 μM was the cytotoxic concentration for inhibition of cell regeneration. In additional, the relationship between drug dose and the cell response (RSI) were fitted, showing that our experimental data were consistent with the dose-response relationship, and the Raman-DIP could be applied for sensitivity testing of targeted anti-cancer drugs. The variation of the bio-macromolecules reflected by the Raman signal in the fingerprint range was closely related to the GSK2334470 concentration. The Raman-DIP method can monitor the inhibition of the GSK2334470 to the MCF-7 cell proliferation with high accuracy as the CCK-8 assay. The combination of Raman-DIP results and fingerprint range information can effectively improve the evaluation, serving as a supplement for the existing anti-cancer drug sensibility assay. This method is valuable to reveal the intracellular macromolecules changes related to the drug treatment.
K E Y W O R D Santi-cancer drug screening, breast cancer, deuterium isotope probing, Raman spectroscopy, single cell level Xia Huang and Chengjian Li contributed equally to this work and should be considered co-first authors.
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