Accuracy of Quantitative Paper Chromatography in Amino Acid Analysis-Addendum

Salander, R. C.; Piano, Marcus; Patton, A. R.

doi:10.1021/ac60080a031

Cited by 19 publications

(5 citation statements)

References 17 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Descending paper chromatography was carried out with upper layer of solvent composed of butanol-acetic acid-water (4:1:5). Chromatograms were sprayed with aniline phthalate (21) and ninhydrin (27). bRetention times were measured at the center of peak.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Characterization of a Cytoplasmic Reserve Glucan from Ruminococcus albus

Cheng¹,

Brown²,

Costerton³

1977

Appl Environ Microbiol

View full text Add to dashboard Cite

Ruminococcus albus , an anaerobic bacterium that digests cellulose in the rumen of cattle, produces intracellular polysaccharide granules varying from 0.05 to 0.31 μm in diameter when grown in batch culture. This polysaccharide material was purified and found to contain d -glucose as the only reducing sugar. The polyglucose polymer was slightly opalescent in aqueous solution and formed a strong reddish purple iodine complex with a maximum absorbance at 550 nm. Its infrared spectrum had characteristic absorption bands at 8.70, 9.25, and 9.75 μm and was identical with that of the amylopectin-glycogen type of Megasphaera elsdenii and that of the glycogen of enteric bacteria and beef liver. It reacted strongly with concanavalin A. Methylation analysis showed that the glucan contained 2,3,4,6-tetra- O -MeG-2,3,6-Tri- O -MeG-2,3-Di- O -MeG in a ratio of 8:84:8. Characterization of the products obtained by treatment with isoamylase indicates that the glucan of R. albus is of the glycogen type.

show abstract

Section: Discussionmentioning

confidence: 99%

“…Aniline phthalate (21) was used to locate the sugar spots. Ninhydrin spray was used to locate ninhydrin-positive materials (27).…”

Section: Methodsmentioning

confidence: 99%

Characterization of a Cytoplasmic Reserve Glucan from Ruminococcus albus

Cheng¹,

Brown²,

Costerton³

1977

Appl Environ Microbiol

View full text Add to dashboard Cite

show abstract

“…Included in these is a "three-point" attachment to the cellulose surface (90). Various quantitative paper chromatographic methods for the separation and determination of amino acids in mixtures are reported in the recent literature (61,141,183,274,301,312,317). Displacement chromatography on ion exchangers has been used also in the separation and determination of amino acids (273).…”

Section: Proteins Amino Acids and Related Compoundsmentioning

confidence: 99%

Review - Pharmaceuticals and Natural Drugs

Marsh¹,

Hilty²

1955

Anal. Chem.

View full text Add to dashboard Cite

“…The rather obvious but neglected technique of scanning paper chromatograms with a high frequency apparatus was described by Hashimoto and Mori (202). Scanning methods with visible and ultraviolet light have been widely used (59,408,433). A number of absorptiometric scanning devices and techniques have been described (74,266,320,353,485) with inclusion of ultraviolet absorption (49,135,190,203,304,379), which has been applied to nucleic acid derivatives (135, 304), ketosteroids (485), and plant phenols (49).…”

Section: Paper Chromatographymentioning

confidence: 99%