ABSTRAKPengukuran morfometrik menggunakan citra digital memberikan beberapa keuntungan, antara lain lebih aman baik untuk peneliti maupun ternak, memberikan data yang lebih akurat, meminimalkan stres pada ternak serta memberikan data yang lebih detail dan lengkap. Penelitian ini menggunakan 20 ekor sapi Peranakan Ongole dan 20 ekor sapi Bali dengan umur rata-rata 3 tahun. Pengambilan citra digital menggunakan kamera DSLR dengan resolusi 18 Mp. Analisis data menggunakan program Image J. Parameter yang diamati adalah panjang badan, tinggi badan, tinggi hips, tinggi pelvis, tinggi loin, panjang Ossa vertebrae cervicales, panjang Ossa vertebrae thoracicae, panjang Ossa vertebrae lumbales, panjang Os scapula, panjang Os humerus, panjang Ossa radius-ulna, panjang Os metacarpale III, panjang Os femoris, panjang Ossa tibia-fibula, and panjang Os metatarsale III. Analisis Citra Digital mampu menggantikan pengukuran morfometrik manual dengan nilai keragaman yang rendah, yaitu di bawah 10. Aplikasi pengukuran pada sapi Peranakan Ongole dan Bali menunjukkan bahwa sapi Bali memiliki potensi deposisi perdagingan pada area sumbu tubuh sedangkan pada sapi Peranakan Ongole potensi deposisi perdagingan ada pada alat gerak depan dan belakang.Kata kunci: citra digital, morfometrik, perbandingan tubuh, sapi Peranakan Ongole, sapi Bali ABSTRACT Morphometric measurements using digital image technique give a lot of benefits i.e., a safer method both for the researchers and the experimental animals, produce a more accurate morphometric data, reduce the stress in the experimental animals and produce a more detail and complete morphometric data. This experiment used 20 Ongole cross cattle and 20 Bali cattle with the average age of 3 years. Digital image data were collected by using DSLR camera with 18Mp resolution. The collected digital image data were analyzed by using Image J program. Parameters measured in this observation were body length, body height, hip height, pelvic height, loin height, the length of ossa vertebrae cervicales, the length of ossa vertebrae thoracicae, the length of ossa vertebrae lumbales, the length of os scapula, the length of os humerus, the length of ossa radius-ulna, the length of os metacarpale III, the length of os femoris, the length of ossa tibia-fibula, and the length of os metatarsale III. Digital image technique could replace the manual technique for morphometric measurement with a lower level of variance (<10). Application of digital image technique for measurement of morphometric parameters in Ongole Cross and Bali cattle showed that Bali cattle has a potential for muscle deposition in body frame and body axis while Ongole Cross cattle has a potential for muscle deposition in fore and hind legs.