Accuracy in the Determination of Recovery in HPLC

Schümann, M.; Mehlis, B.; Flentje, H.

doi:10.1080/01483919108049692

Cited by 2 publications

(1 citation statement)

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“…Although the recovery is sometimes quite difficult to assess due to the interference of other endogenous compounds in sample matrices, spiking standards during method development were done by spiking the standards into a blank and carrying through the extraction. Our results showed a variation of GLS recovery between 89.0 and 108.6% ( Table 3 ), which according to literature [ 23 , 25 , 26 ] is acceptable and indicates good accuracy of the proposed HPLC method. In addition, the method showed good selectivity, since all GLS were well separated from each other with good resolution ( Figure 2 ).…”

Section: Resultssupporting

confidence: 84%

Rapid Separation of Indole Glucosinolates in Roots of Chinese Cabbage (Brassica rapaSubsp. Pekinensis) by High-Performance Liquid Chromatography with Diode Array Detection

Aires

Carvalho

2017

International Journal of Analytical Chemistry

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Glucosinolates are a class of sulphur-containing plant compounds with diverse biological properties. They have been found exclusively in the Brassicaceae family plants and studied exhaustively in biosynthetic and application perspectives. The aim of this current study is to present a simple and updated method to quantify indole glucosinolate content in hairy root cultures of Chinese cabbage by HPLC-DAD-UV/Vis. Method validation controls were performed and recovery experiments were assayed. The data was statically treated and compared with published works. The current method allowed a feasible identification of indole glucosinolates and it was possible to identify accurately three indole glucosinolate compounds (glucobrassicin, 4-methoxyglucobrassicin, and 1-methoxyglucobrassicin) in roots of Chinese cabbage. The method demonstrated a good linearity (R2 > 0.99), a good precision, and selectivity sensitivity. In conclusion, this protocol provides an accessible method to extract and quantify glucosinolates in plant samples. Thus, based on our results, the method is valid and can be extended to other plant or food matrices.

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Section: Resultssupporting

confidence: 84%

Rapid Separation of Indole Glucosinolates in Roots of Chinese Cabbage (Brassica rapaSubsp. Pekinensis) by High-Performance Liquid Chromatography with Diode Array Detection

Aires

Carvalho

2017

International Journal of Analytical Chemistry

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High Performance Liquid Chromatographic Analyses of Sulphonamides and Dihydrofolate Reductase Inhibitors. IV. Recoveries from the Stationary Phase, Quantitation and Sensitivities

Ricci

Cross

1996

Journal of Liquid Chromatography & Related Technologies

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Of the twenty-two sulphonamides and three commonly used dihydrofolate reductase inhibitors investigated for reverse phase separation in previous studies, the recoveries of five representative drugs from the stationary phase have been examined. Sulphanilamide, sulfisomindine, sulphaquinoxaline, diaveridine and pyrimethamine were chosen. Peak areas off the analytical column were compared with those obtained by substitution with stainless steel tubing. After careful correction for flow rate differences where necessary, 100% recoveries were indicated. As a further check, Maloprim tablets were assayed for pyrimethamine and the analysed results were found to be (99.1 f 3155 COpyight C 1996 by Marcel Dekker, Inc. Downloaded by [Michigan State University] at 23:01 08 February 2015 3 156 RICCI AND CROSS 2.9)%. Limits of detection (LOD) for aqueous standards varied from 0.67 (for sulphanilic acid) -0.03 pg mL-' (diaveridine).The linear dynamic range extended from the LOD for each drug to >5 pg mL-' .

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Accuracy in the Determination of Recovery in HPLC

Cited by 2 publications

References 5 publications

Rapid Separation of Indole Glucosinolates in Roots of Chinese Cabbage (Brassica rapaSubsp. Pekinensis) by High-Performance Liquid Chromatography with Diode Array Detection

Rapid Separation of Indole Glucosinolates in Roots of Chinese Cabbage (Brassica rapaSubsp. Pekinensis) by High-Performance Liquid Chromatography with Diode Array Detection

High Performance Liquid Chromatographic Analyses of Sulphonamides and Dihydrofolate Reductase Inhibitors. IV. Recoveries from the Stationary Phase, Quantitation and Sensitivities

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