Accuracy in spectrophotometry and luminescence measurements

Mavrodineanu, R.; Shultz, J I; Menis, Oscar

doi:10.6028/nbs.sp.378

Cited by 12 publications

(9 citation statements)

References 25 publications

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“…The entrance slit of the monochromator was equipped with a mechanical shutter, the single axial Model 227MCD (JY Horiba). Appendix A describes the procedures used to validate the performance of the fluorometer [4]. …”

Section: Calibration Of the Fluorometermentioning

confidence: 99%

“…The lowest point (for a concentration of approximately 7 pmol/L) was systematically lower than expected from the linear trend set by the higher points. We rationalize this as an effect of adsorption of fluorescein on the capillary walls [4]. A calibration was accepted if the slope of the best fit fell between 0.95 and 1.05.…”

Section: Calibration Of the Fluorometermentioning

confidence: 99%

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Quantitating fluorescence intensity from fluorophore: Assignment of MESF values

Gaigalas¹,

Wang²,

Schwartz³

et al. 2005

J. Res. Natl. Inst. Stand. Technol.

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A procedure is presented to convert the comparison of measured fluorescence signals into a comparison of fluorescence yields (FY). The fluorescence yield, which is a property of a solution or a suspension, is defined as the product of the fluorophore concentration and the molecular quantum yield. The paper revises the measurement model which relates the measured fluorescence signal to the FY. The equality of FY of two solutions provides an equivalence between the concentrations of fluorophore in the two solutions. The equivalence is the basis for quantitation in terms of molecules of equivalent soluble fluorophore (MESF). The quantitation procedure starts with the measurement of fluorescence signals from a serial dilution of fluorescein solutions to obtain a calibration of a fluorometer. The fluorometer is used to measure the fluorescence signal of a suspension of microspheres with immobilized fluorescein isothiocyanate (FITC). The calibration is used to obtain the concentration of soluble fluorophores which gives the same fluorescence signal as the microsphere suspension. The number concentration of microspheres is measured and the equality of fluorescence yields is used to obtain the number of soluble fluorescein molecules equivalent to a single microsphere.

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Section: Calibration Of the Fluorometermentioning

confidence: 99%

Section: Calibration Of the Fluorometermentioning

confidence: 99%

Quantitating fluorescence intensity from fluorophore: Assignment of MESF values

Gaigalas¹,

Wang²,

Schwartz³

et al. 2005

J. Res. Natl. Inst. Stand. Technol.

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“…In 1953 the author did this for ZEISS's ELKO II photometer. 2 The apparent errors according to eq (36) were eliminated together with the apparent errors from eq (12) which depend similarly on the transmittance, and possible genuine linearity errors (17). They were eliminated by so-called correctors described by Hansen [24] (figs.…”

Section: 1ti=-t/ · (L-t/)'{ R2+(ra+rb)r+rarb}' (36)mentioning

confidence: 99%

“…The study is limited to photometers for UV, VIS, and near IR. A good survey of the literature published until 1972, especially regarding tests for photometric linearity, is included in the proceedings of the Conference on Accuracy in Spectrophotometry and Luminescence Measurements, held at the National Bureau of Standards in March 1972 [2], particularly in the contributions of Clarke [3], Mavrodineanu [4], and Sanders [5].…”

Section: Introductionmentioning

confidence: 99%

Errors in spectrophotometry and calibration procedures to avoid them

Reule¹

1976

J. RES. NATL. BUR. STAN. SECT. A.

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. Based o~ simple principles, s l~ectrop hotoI?ctry nevertheless demands a lot of precautIOns to aVOi d errors. The followmg properties of spectrophotometers will be discussed together with methods to test them:~pectra~ properties-wavelength accuracy, bandwidth, stray light; photometri c linearity; mteractlOns between sample a~d instrument-multiple reflections, polarization, divergence, sample wedge, sample tIlt, optical path length (refr active index), interferences .Calibratio~ of master instruments is feasiJ:>le only by complicated procedures. With such a master mstrument standards may be calIbrated which greatly simplify performance checks of mst:u:nen~s used for practical work. For testing high quality spcctrophotometcrs the u~e of emiSSIOn lmes and nearly neutral absorbing solid filters as standards seems to be supenor, for some kinds of routine instruments the use of absorption bands and liquid filters may be necessary.

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“…4AAA,3rd ed. (673); an English translation of "Photometric Analysis; General Principles and Working Tools" (54), and "Accuracy in Spectrophotometry and Luminescence Measurements" (447).…”

mentioning

confidence: 99%